FastQCFastQC Report
Mon 6 Feb 2017
SRR2077286_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077286_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3994653
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46000.032.9537051
GTATCAA87950.032.0370031
TCAACGC112750.024.6948094
TATCAAC113900.024.5348132
ATCAACG113500.024.53043
CAACGCA114250.024.2812125
AACGCAG119800.023.1926926
ACGCAGA139500.019.7217397
GTACATG136250.019.2861561
CGCAGAG142300.019.1173088
AGAGTAC132000.019.01783411
TACATGG136700.018.8274632
GAGTACT68550.018.3611512
TATACCG1702.4325836E-618.2668695
ACGCGAT950.00786021118.14888811
ACATGGG141900.017.50563
GTGGTAT24700.017.3349361
CAGAGTA148200.017.31037910
GCAGAGT151450.017.2576529
GTACTTT73850.016.904414
Produced by FastQC (version 0.11.5)