FastQCFastQC Report
Mon 6 Feb 2017
SRR2077286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3994653
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57000.035.0845341
GTATCAA110100.034.0332871
TATCAAC149600.024.8690912
ATCAACG149050.024.802523
TCAACGC150500.024.5399654
CAACGCA152050.024.3701745
AACGCAG158800.023.2664226
ACGCAGA182450.020.060017
GTACATG130700.020.0128521
CGCAGAG186450.019.4817498
TACATGG132350.019.4369562
AGAGTAC177850.019.31742711
GAGTACT117100.018.48803312
ACATGGG136450.018.1717073
CAGAGTA194350.017.99719810
GCAGAGT199650.017.7959299
AGTACTT124250.017.63915413
GTACTTT124400.017.46996914
TCGCACG2502.0294465E-816.56691613
CGATACG1250.001946410416.55405656
Produced by FastQC (version 0.11.5)