FastQCFastQC Report
Mon 6 Feb 2017
SRR2077285_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077285_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5523514
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42250.035.222071
GTATCAA81450.033.404071
TCAACGC100150.026.424164
ATCAACG101500.026.1064623
CAACGCA102400.025.7429355
TATCAAC106000.025.6477662
AACGCAG107500.024.6276786
ACGCAGA122500.021.3323787
CGCAGAG127150.020.4725638
GTACATG123750.020.39561
GCGATAC4300.020.05041914
AGAGTAC124050.019.70673611
TACATGG125250.019.2818872
ACATGGG129500.018.0905953
CAGAGTA139450.017.85171710
GTACCGT5800.017.8305196
GAGTACT72900.017.64210912
GTATAGC19450.017.5743751
CGTGCCG5050.017.06907310
GCAGAGT149150.016.898639
Produced by FastQC (version 0.11.5)