FastQCFastQC Report
Mon 6 Feb 2017
SRR2077285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4723778
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48700.040.2843741
GTATCAA90550.033.2822151
ATCAACG125550.023.4929813
TATCAAC126650.023.478312
TCAACGC125700.023.4649474
CAACGCA128650.022.8699055
AACGCAG134250.021.939096
GCGATAC3550.020.405714
GTACATG111750.019.413441
ACGCAGA153050.018.9732597
CGAATCG3350.018.53089137
TACATGG111900.018.508462
CGCAGAG156350.018.4191888
CGTGCCG4950.018.13778510
GTGGTAT24450.018.113971
GAGTACT109750.017.55681812
AGAGTAC155450.017.43793511
ACATGGG117300.017.215543
TCGATCG2702.9431249E-916.60500541
AGTACTT118350.016.5331213
Produced by FastQC (version 0.11.5)