FastQCFastQC Report
Mon 6 Feb 2017
SRR2077284_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077284_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5033998
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38900.034.4397741
GTATCAA79100.034.2229731
TCAACGC102100.025.9187854
ATCAACG102550.025.8718263
CAACGCA103750.025.5073455
TATCAAC105600.025.2871842
AACGCAG109350.024.0855486
ACGCAGA124750.020.9485427
GTACATG124000.020.8006571
TACATGG124200.020.0279772
CGCAGAG129650.019.998858
AGAGTAC128650.019.24383711
GAGTACT79500.018.78028112
ACATGGG129500.018.7026753
CAGAGTA140250.017.8239310
AGTACTT88650.017.0781413
GTACTTT88100.017.06715214
GCAGAGT147350.017.0585239
GTATAGC16450.016.7919921
CATGGGG90100.015.6235754
Produced by FastQC (version 0.11.5)