FastQCFastQC Report
Mon 6 Feb 2017
SRR2077284_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077284_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5033998
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48250.038.6641121
GTATCAA101900.036.1056981
TATCAAC140650.025.796852
ATCAACG140400.025.770423
TCAACGC140850.025.7121324
CAACGCA143200.025.2258075
AACGCAG150550.024.1046376
GTGGTAT23500.020.6194361
ACGCAGA174350.020.6161427
CGCAGAG179100.020.0320078
GTACATG119250.019.6202681
AGAGTAC180100.019.15500311
TACATGG120400.018.9604382
GAGTACT132250.018.8004112
CAGAGTA192550.018.1673710
AGTACTT140500.017.88366913
GCAGAGT197500.017.8513839
GTACTTT140700.017.5796714
ACATGGG125300.017.2262363
TGGTATC26250.017.129512
Produced by FastQC (version 0.11.5)