FastQCFastQC Report
Mon 6 Feb 2017
SRR2077283_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077283_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4378557
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44800.030.5923481
GTATCAA83350.030.1112731
ATCAACG103150.024.0780033
TCAACGC103450.024.0423494
TATCAAC105700.023.594492
CAACGCA106450.023.4941375
AACGCAG110400.022.6994066
AGAGTAC117200.019.59296211
ACGCAGA127000.019.5708877
CGCAGAG128750.019.1731938
GAGTACT75950.019.11250912
GTACATG102350.018.2814961
CAGAGTA132750.017.71284910
TACATGG101400.017.4512542
AGTACTT84550.017.41475713
GTACTTT84150.017.37460114
GTATAGC15900.017.3697341
TAGCCCT28400.017.1266694
TACACCG4650.017.0624755
GCAGAGT139750.016.9243059
Produced by FastQC (version 0.11.5)