FastQCFastQC Report
Mon 6 Feb 2017
SRR2077283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4378557
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52050.035.5569041
GTATCAA100250.032.6090621
ATCAACG131850.024.389043
TATCAAC133700.024.1533582
TCAACGC134050.023.8858684
CAACGCA136400.023.4157435
AACGCAG141550.022.5627776
ACGCAGA164550.019.1987977
AGAGTAC160250.018.81099111
CGCAGAG167700.018.797868
GTACATG94700.018.6664521
TACATGG94750.018.3183572
GAGTACT123550.018.2221812
CAGAGTA178350.017.23107310
AGTACTT131100.017.16629213
GTACTTT132100.016.9757414
ACATGGG98400.016.9724253
GCAGAGT183550.016.9678869
CCTATAC9300.016.3591823
TACTTTT150550.014.93947615
Produced by FastQC (version 0.11.5)