FastQCFastQC Report
Mon 6 Feb 2017
SRR2077282_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077282_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4604867
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41700.034.7695661
GTATCAA86000.034.481071
TCAACGC108150.027.0499784
ATCAACG108500.026.8028663
TATCAAC109900.026.5532862
CAACGCA110650.026.501755
AACGCAG115400.025.2712446
ACGCAGA130850.022.2358867
CGCAGAG135950.021.2763358
AGAGTAC128600.020.85885611
GTACATG117500.020.418931
GAGTACT79200.019.85098612
TACATGG119100.019.3758142
CAGAGTA141800.019.35395610
GCAGAGT147350.018.8349519
ACATGGG122000.018.1817023
GTACTTT85550.018.17701514
AGTACTT87650.017.78046413
GTGGTAT23000.015.7596841
GTATAGG11200.015.7194791
Produced by FastQC (version 0.11.5)