FastQCFastQC Report
Mon 6 Feb 2017
SRR2077282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4604867
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48150.040.809811
GTATCAA103350.036.85441
TATCAAC143550.026.3763872
TCAACGC145900.026.0737344
ATCAACG146250.025.940963
CAACGCA148500.025.5084695
AACGCAG153450.024.6368586
ACGCAGA178900.020.9974277
CGCAGAG183800.020.3812948
AGAGTAC180850.019.60711311
GAGTACT129200.019.29462612
CAGAGTA192150.018.6696810
GTGGTAT22400.018.378561
GCAGAGT200750.018.196279
AGTACTT138150.018.0365913
GTACATG115550.017.9336781
GTACTTT139900.017.90128114
TACATGG117050.017.2227122
TGGTATC23400.016.8458672
TACTTTT154500.016.2530615
Produced by FastQC (version 0.11.5)