FastQCFastQC Report
Mon 6 Feb 2017
SRR2077281_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077281_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4037779
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40200.032.466931
GTATCAA76350.031.6566161
TCAACGC93850.025.181484
ATCAACG94350.025.1567943
TATCAAC96200.024.8517022
CAACGCA97300.024.2179955
AACGCAG100150.023.5775226
ACGCAGA116200.020.2039977
GAGTACT63850.019.71028512
GCGCGTA1056.041163E-419.70721424
CGCAGAG120050.019.470878
AGAGTAC113550.019.22106611
GTACATG108200.018.5087131
TACATGG108200.018.0781422
GTATAGG8950.017.74641
AGTACTT71800.017.67435513
CAGAGTA127000.017.64589110
GTACTTT72500.017.17117914
GCAGAGT132400.017.0561859
GTACCGT4550.016.6674776
Produced by FastQC (version 0.11.5)