FastQCFastQC Report
Mon 6 Feb 2017
SRR2077281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4037779
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56800.035.5643541
GTATCAA110000.033.269281
TATCAAC144800.024.928352
ATCAACG146050.024.6458113
TCAACGC149000.024.0882384
CAACGCA151800.023.5889235
AACGCAG156350.022.962166
GTGGTAT30050.020.1439191
ACGCAGA179200.019.782337
GAGTACT128250.019.54216612
TGGTATC30750.019.3394852
CGCAGAG182500.019.3310098
GTACATG104750.019.0864091
CGATCGT2354.620233E-1019.07871642
AGAGTAC178250.018.92162711
AGTACTT134200.018.77117713
TACATGG105900.018.4791532
TACACCG3750.018.4161955
GTACTTT137950.018.00607114
TCGATCG2307.3068804E-917.99464241
Produced by FastQC (version 0.11.5)