FastQCFastQC Report
Mon 6 Feb 2017
SRR2077280_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077280_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5122996
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37050.033.6382141
GTATCAA71950.033.3958471
TCAACGC85700.027.1736454
ATCAACG86300.026.9839273
CAACGCA88200.026.208865
TATCAAC92300.025.415472
AACGCAG93150.024.9065256
CCGTACG750.00200038822.9872679
ACGCAGA108650.021.0702747
CGCAGAG112200.020.3744778
GTACATG110500.019.6205581
TACATGG107250.019.5567742
AGAGTAC114650.018.79756211
GTATAGC16450.018.4684621
GAGTACT72400.018.38436112
ACATGGG110550.017.7568423
CAGAGTA124800.017.62725310
GCAGAGT131300.016.9909679
GTACTTT79700.016.78864714
AGTACTT81000.016.3484213
Produced by FastQC (version 0.11.5)