FastQCFastQC Report
Mon 6 Feb 2017
SRR2077280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5122996
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45350.037.7654081
GTATCAA89600.034.56061
ATCAACG119050.025.6165453
TCAACGC120150.025.3817714
TATCAAC123350.025.0867272
CAACGCA122100.024.7176935
AACGCAG128350.023.5069436
CGTGCCG3400.020.30832710
ACGCAGA148900.020.1239917
CGCAGAG152750.019.572158
AGAGTAC154450.018.64255511
GTGGTAT23050.018.6130281
GAGTACT112800.018.1804512
GTACATG105450.017.5538941
CAGAGTA165800.017.55359510
TACATGG103400.017.3877332
GCAGAGT172050.017.2162559
CGAATCG3054.7293724E-1116.96218537
AGTACTT120700.016.8686813
GTACTTT122200.016.68398914
Produced by FastQC (version 0.11.5)