FastQCFastQC Report
Mon 6 Feb 2017
SRR2077279_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077279_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4323963
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80050.033.1761471
GGTATCA39650.029.004271
ATCAACG94650.026.903193
TCAACGC95650.026.513094
CAACGCA98700.025.7980655
TATCAAC101900.025.631242
AACGCAG102550.024.839576
ACGCAGA117800.021.5386317
CGCAGAG119650.021.0639848
AGAGTAC116000.020.18286911
GAGTACT74150.020.08878912
GTATAGC13150.019.4342121
GTACATG97950.018.9334951
AGTACTT80800.018.65097613
GCAGAGT131350.018.5844259
CAGAGTA129600.018.54273210
GTACTTT82400.018.20575114
TACATGG99450.018.1791842
ACATGGG103450.016.6098963
TATACCG2552.6018824E-816.236375
Produced by FastQC (version 0.11.5)