FastQCFastQC Report
Mon 6 Feb 2017
SRR2077279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4323963
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52250.034.8816151
GTATCAA101300.032.330531
ATCAACG131250.024.7044013
TCAACGC132150.024.5361544
TATCAAC133600.024.3199122
CAACGCA137350.023.525535
AACGCAG140800.023.0261066
ACGCAGA163250.019.6694567
CGCAGAG164700.019.4133348
AGAGTAC160950.018.81509411
GAGTACT124350.018.60487712
CGTACAC3350.018.5737043
GTACATG95600.018.5218491
TACATGG94200.018.2366942
AGTACTT130450.017.69940213
CAGAGTA175450.017.496310
GCAGAGT179300.017.3511099
GTACTTT133800.017.22348614
GTATAGC13550.017.100461
ACATGGG100350.016.4312953
Produced by FastQC (version 0.11.5)