FastQCFastQC Report
Mon 6 Feb 2017
SRR2077278_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077278_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4099111
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65300.031.1893021
GGTATCA35650.030.5012631
TCAACGC82400.024.4064944
TATCAAC82850.024.2730392
ATCAACG83300.024.14283
CAACGCA85200.023.5225665
AACGCAG89350.022.4915626
GTACATG110500.020.868011
TACATGG111150.020.544552
ACGCAGA102850.019.3396667
ACATGGG115300.018.938193
CGCAGAG106800.018.5302728
AGAGTAC101700.017.73140311
GAGTACT54000.017.36793112
CATGGGG80600.017.0338234
TCGATCG3157.6397555E-1116.43151341
CAGAGTA114800.016.18859910
GCAGAGT119600.015.88464459
GTGTAGC20450.015.867241
CGATCGT2855.97538E-915.74042942
Produced by FastQC (version 0.11.5)