FastQCFastQC Report
Mon 6 Feb 2017
SRR2077278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4099111
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45300.038.0270081
GTATCAA84350.032.56091
TATCAAC114050.023.7941232
ATCAACG116750.023.337253
TCAACGC117250.023.148984
CAACGCA119450.022.779935
AACGCAG124800.021.8850566
GTACATG108450.018.914121
GTGGTAT22450.018.797691
ACGCAGA147150.018.4427997
TACATGG108050.018.3646412
CGCAGAG149350.018.1024368
GAGTACT95850.017.8684112
AGAGTAC145400.017.6687111
AGTACTT99950.017.19529213
GTACTTT99750.017.18866514
ACATGGG112150.016.8951343
CAGAGTA156150.016.85036810
AACGGGG10300.016.74290319
TACACCG4400.016.4808245
Produced by FastQC (version 0.11.5)