FastQCFastQC Report
Mon 6 Feb 2017
SRR2077277_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077277_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13113
Sequences flagged as poor quality0
Sequence length75
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA509.767973E-1055.21
GTATCAA1550.044.5161321
AGAGCAC350.001299671439.4285745
CACTTTT350.001299671439.4285749
AGCACTT350.001299671439.4285747
GCACTTT350.001299671439.4285748
GACTTTT501.7631656E-434.515
TATCAAC1950.031.8461552
TCAACGC2250.030.6666684
ATCAACG2250.030.6666683
GAGCACT450.0044595430.6666666
CAACGCA2150.028.883725
GAGGACT500.007463575327.612
AGAGGAC500.007463575327.611
AACGCAG2350.026.4255316
CGCAGAG2650.024.735858
ACGCAGA2600.023.8846157
GCAGAGT1952.7284841E-1123.09
GTACTTT2205.456968E-1221.95454614
CAGAGTA1957.0940587E-1021.2307710
Produced by FastQC (version 0.11.5)