FastQCFastQC Report
Mon 6 Feb 2017
SRR2077276_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077276_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4977075
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41650.034.9827161
GTATCAA83950.034.053811
ATCAACG104050.026.5243783
TCAACGC104500.026.443974
CAACGCA106150.025.967925
TATCAAC106850.025.9574092
AACGCAG111700.024.840466
ACGCAGA128100.021.6348787
CGCAGAG131050.021.0982328
CGAATCG4100.020.2008237
AGAGTAC128850.019.93436611
GTACATG111600.019.8003041
TACATGG110150.019.4485362
CAGAGTA140600.018.66059710
GAGTACT86450.018.62515612
GCAGAGT146850.018.0540319
ACATGGG116800.017.9284653
GTACTTT93750.016.99253314
AGTACTT97400.016.81529813
GTGTAGC26550.016.1255041
Produced by FastQC (version 0.11.5)