FastQCFastQC Report
Mon 6 Feb 2017
SRR2077276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4977075
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51000.039.964981
GTATCAA102100.034.3674051
TATCAAC139450.024.7477512
ATCAACG140650.024.2936863
TCAACGC143800.023.8093834
CAACGCA144450.023.691815
AACGCAG150950.022.7121246
ACGCAGA175450.019.3640637
CGTGCCG4850.019.22122810
CGCAGAG179350.018.9237378
GTACATG105550.018.8514211
GAGTACT137850.018.40940312
AGAGTAC178750.018.31139411
TACATGG105500.017.9618822
GTACTTT146750.017.48936314
GTGGTAT26150.017.4677411
AGTACTT147450.017.3899313
CAGAGTA193150.017.07134610
GCAGAGT198400.016.9152859
ACATGGG110100.016.6481043
Produced by FastQC (version 0.11.5)