FastQCFastQC Report
Mon 6 Feb 2017
SRR2077275_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077275_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4590914
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79900.032.6681061
GGTATCA42050.029.8871361
TCAACGC100050.025.552724
TATCAAC102200.025.2841262
ATCAACG102750.025.0827273
CAACGCA102550.024.8616855
AACGCAG108900.023.4278186
ACGCAGA123250.020.4492537
GTACATG115650.020.3604641
AGAGTAC119100.019.68797111
CGCAGAG129100.019.550828
TACATGG118300.019.4517272
GAGTACT68800.018.69535312
ACATGGG125800.017.8814583
CAGAGTA133950.017.76216710
GCAGAGT139550.017.4941569
AGTACTT76450.016.91628313
GTATAGG11350.016.7307621
GTACTTT80300.016.14835214
CATGGGG93350.015.153244
Produced by FastQC (version 0.11.5)