FastQCFastQC Report
Mon 6 Feb 2017
SRR2077275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4590914
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52750.035.1471671
GTATCAA102000.033.335071
ATCAACG139050.024.0193
TATCAAC140650.023.9392812
TCAACGC140900.023.7286914
CAACGCA143400.023.214035
AACGCAG147550.022.6726886
GTACATG117700.019.5724771
ACGCAGA171800.019.3731737
TACATGG120800.018.9158742
AGAGTAC166700.018.86939811
GAGTACT115350.018.67851312
CGCAGAG179300.018.4286168
ACATGGG124400.018.1207433
TATACCG2952.910383E-1117.5586815
CAGAGTA183450.017.391210
AGTACTT122650.017.1948113
GCAGAGT189950.017.0318349
GTACTTT127800.016.49363314
CATGGGG93150.015.66346554
Produced by FastQC (version 0.11.5)