FastQCFastQC Report
Mon 6 Feb 2017
SRR2077274_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077274_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5126515
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95150.031.2435531
GGTATCA52650.030.7567561
TCAACGC120800.024.3334244
ATCAACG121050.024.1976623
TATCAAC122350.024.081082
CAACGCA123250.023.8231185
AACGCAG126900.023.067066
ACGCAGA148600.019.58437
GTACATG133000.019.4963971
TACATGG133250.019.3667352
AGAGTAC142100.018.95362311
CGCAGAG152650.018.9308368
GAGTACT85000.017.89201412
ACATGGG139150.017.6533573
CAGAGTA160550.017.14033910
GCAGAGT169300.016.4785279
CGAATCG3006.8030204E-1016.10294337
AGTACTT94650.016.03109413
GTACTTT94750.015.868914
GTATAGC15800.015.2970251
Produced by FastQC (version 0.11.5)