FastQCFastQC Report
Mon 6 Feb 2017
SRR2077274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5126515
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120750.034.0438271
GGTATCA62800.033.6659281
ATCAACG161050.025.2757363
TATCAAC162750.025.0725272
TCAACGC164250.024.8462284
CAACGCA166850.024.3178255
AACGCAG174200.023.3683386
ACGCAGA200150.020.0615797
CGCAGAG205000.019.5369978
AGAGTAC195650.019.41240111
GAGTACT144650.019.33450112
GTACATG124750.019.222141
AGTACTT152050.018.49857513
GTACTTT154850.018.1782114
CAGAGTA215000.017.93829310
TACATGG130500.017.5157222
GCAGAGT224700.017.3940729
ACATGGG130650.016.7299923
TACTTTT173200.016.1114515
CGCGTAA1300.002509879615.93581610
Produced by FastQC (version 0.11.5)