##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077274_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5126515 Sequences flagged as poor quality 0 Sequence length 75 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.90590293796078 31.0 31.0 34.0 27.0 34.0 2 31.002514378676352 33.0 31.0 34.0 27.0 34.0 3 31.083181264465235 33.0 31.0 34.0 27.0 34.0 4 34.43440504904404 37.0 35.0 37.0 31.0 37.0 5 34.152815119042856 37.0 35.0 37.0 30.0 37.0 6 34.130574279018006 37.0 35.0 37.0 30.0 37.0 7 33.964553112592085 37.0 35.0 37.0 30.0 37.0 8 33.92185783129475 37.0 35.0 37.0 29.0 37.0 9 35.398648204481994 39.0 37.0 39.0 29.0 39.0 10 35.29301211446763 39.0 35.0 39.0 28.0 39.0 11 35.28757099120943 39.0 35.0 39.0 28.0 39.0 12 35.18602598451385 39.0 35.0 39.0 27.0 39.0 13 35.12154787414062 39.0 35.0 39.0 27.0 39.0 14 36.362576526158605 40.0 37.0 41.0 27.0 41.0 15 36.41034679504498 40.0 37.0 41.0 28.0 41.0 16 36.39535122788093 40.0 37.0 41.0 27.0 41.0 17 36.39079452610594 40.0 37.0 41.0 27.0 41.0 18 36.398105535631906 40.0 37.0 41.0 28.0 41.0 19 36.43674191921803 40.0 37.0 41.0 27.0 41.0 20 36.40336719974486 40.0 37.0 41.0 27.0 41.0 21 36.38140335100941 40.0 37.0 41.0 27.0 41.0 22 36.36082114262808 40.0 37.0 41.0 27.0 41.0 23 36.32929387702952 40.0 37.0 41.0 27.0 41.0 24 36.31179895113932 40.0 37.0 41.0 27.0 41.0 25 36.28246167230565 40.0 37.0 41.0 27.0 41.0 26 36.21763303140633 40.0 36.0 41.0 27.0 41.0 27 36.16303141607895 40.0 36.0 41.0 27.0 41.0 28 36.14424574979299 40.0 36.0 41.0 27.0 41.0 29 36.09166500049254 40.0 36.0 41.0 27.0 41.0 30 36.01492495389168 40.0 36.0 41.0 27.0 41.0 31 35.9275117696915 40.0 36.0 41.0 27.0 41.0 32 35.8568343211714 40.0 36.0 41.0 27.0 41.0 33 35.79520297902181 40.0 35.0 41.0 27.0 41.0 34 35.73131298747785 40.0 35.0 41.0 26.0 41.0 35 35.64553366175657 40.0 35.0 41.0 26.0 41.0 36 35.58986192374352 40.0 35.0 41.0 25.0 41.0 37 35.531947921736304 40.0 35.0 41.0 25.0 41.0 38 35.47434329169036 40.0 35.0 41.0 25.0 41.0 39 35.401251337409526 40.0 35.0 41.0 25.0 41.0 40 35.33965666734614 40.0 35.0 41.0 25.0 41.0 41 35.291354068016965 40.0 35.0 41.0 25.0 41.0 42 35.230755981402574 40.0 35.0 41.0 24.0 41.0 43 35.132684679553265 40.0 35.0 41.0 24.0 41.0 44 35.006579518444795 40.0 35.0 41.0 24.0 41.0 45 34.91289911372541 40.0 35.0 41.0 23.0 41.0 46 34.78430161620516 39.0 35.0 41.0 22.0 41.0 47 34.63834359208936 39.0 35.0 41.0 21.0 41.0 48 34.532212623975546 39.0 34.0 41.0 20.0 41.0 49 34.445208489587955 39.0 34.0 41.0 19.0 41.0 50 34.44200163268809 39.0 34.0 41.0 18.0 41.0 51 34.37247779436908 39.0 34.0 41.0 18.0 41.0 52 34.307544794075504 39.0 34.0 41.0 18.0 41.0 53 34.21496708777796 39.0 34.0 41.0 16.0 41.0 54 34.182666002147656 39.0 34.0 41.0 15.0 41.0 55 34.1044120616052 39.0 34.0 41.0 15.0 41.0 56 33.98273993151293 39.0 34.0 41.0 14.0 41.0 57 33.91219590696604 39.0 34.0 41.0 13.0 41.0 58 33.81623734642345 39.0 34.0 41.0 13.0 41.0 59 33.72873911419356 38.0 34.0 41.0 12.0 41.0 60 33.703950539499054 38.0 34.0 41.0 10.0 41.0 61 33.59230471382606 38.0 34.0 41.0 8.0 41.0 62 33.45171388360319 38.0 34.0 40.0 7.0 41.0 63 33.32023567667314 37.0 34.0 40.0 6.0 41.0 64 33.17110727267939 37.0 33.0 40.0 2.0 41.0 65 33.04471283123135 37.0 33.0 40.0 2.0 41.0 66 32.848873942629645 37.0 33.0 40.0 2.0 41.0 67 32.65756561718828 37.0 33.0 40.0 2.0 41.0 68 32.448660737362516 36.0 33.0 39.0 2.0 41.0 69 32.28576469589965 36.0 33.0 39.0 2.0 41.0 70 32.09469981069011 36.0 33.0 39.0 2.0 41.0 71 31.913705119364714 36.0 33.0 39.0 2.0 41.0 72 31.712909647196977 35.0 32.0 39.0 2.0 41.0 73 31.51462133632692 35.0 32.0 39.0 2.0 40.0 74 31.253767715494835 35.0 32.0 38.0 2.0 40.0 75 31.031128164064672 35.0 32.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4642071514970638 1101 2 0.5162333881123295 1101 3 0.49751947311388633 1101 4 0.41480305171785403 1101 5 0.5357509634246185 1101 6 0.5156262599792285 1101 7 0.5637072630200777 1101 8 0.5416761750991554 1101 9 0.7364454777231586 1101 10 0.6772948271409618 1101 11 0.7242794584742 1101 12 0.7890935160491495 1101 13 0.7979960050390815 1101 14 0.9313675595549427 1101 15 0.8356332141741447 1101 16 0.8260270063216595 1101 17 0.8522563386308661 1101 18 0.8240696940818708 1101 19 0.8944197806278709 1101 20 0.8560208301255727 1101 21 0.85862566246103 1101 22 0.8387770010126374 1101 23 0.8823398985814848 1101 24 0.9041548171300633 1101 25 0.9001488351774682 1101 26 1.0087656795953066 1101 27 1.0590043601427226 1101 28 1.0244400765010013 1101 29 0.9731718189378924 1101 30 0.9389704763691569 1101 31 0.958511802443681 1101 32 0.934669061262241 1101 33 0.8562866338065618 1101 34 0.8954204334310063 1101 35 0.9564633893591363 1101 36 0.9225464571525706 1101 37 0.9613891188971806 1101 38 1.0118794902280754 1101 39 1.0460000372299518 1101 40 1.0454440671383765 1101 41 0.9441710258564626 1101 42 1.0074871237276923 1101 43 1.031480668022013 1101 44 1.0348229622390477 1101 45 1.0678668373162168 1101 46 1.0852572956462652 1101 47 1.043357417313537 1101 48 1.0842880334575753 1101 49 1.047598117299266 1101 50 0.9475562475686132 1101 51 0.97453143064633 1101 52 0.8789172274028445 1101 53 0.9670267428891108 1101 54 0.9870064814808401 1101 55 0.9695359621189894 1101 56 1.009862750942247 1101 57 0.9971047112152078 1101 58 0.9635242807398612 1101 59 0.9504618512606342 1101 60 0.9845777597898575 1101 61 0.9231560265019993 1101 62 0.929154846513562 1101 63 0.9572822733227397 1101 64 0.9398298281966362 1101 65 0.8364566728998568 1101 66 0.8782896496513644 1101 67 0.9485892079688298 1101 68 0.9603351893607339 1101 69 0.9265210308216254 1101 70 0.8698220246654174 1101 71 0.8682474965426188 1101 72 0.9113587587994587 1101 73 0.9486228670539134 1101 74 1.0058347968859351 1101 75 0.9752920876242861 1102 1 0.6683548137862907 1102 2 0.6761707564772763 1102 3 0.6830235931250357 1102 4 0.6645510051637231 1102 5 0.7326471589172385 1102 6 0.791969454570939 1102 7 0.7748192456730862 1102 8 0.8077560390511849 1102 9 0.9606570293580603 1102 10 0.9620208816253921 1102 11 0.936771938272642 1102 12 0.9780191806432796 1102 13 0.9602029273572867 1102 14 1.153906507389209 1102 15 1.054911992795894 1102 16 1.1487075054511777 1102 17 1.0618762191349091 1102 18 1.1151539970125768 1102 19 1.152221790072808 1102 20 1.0841782386632772 1102 21 1.147795193445944 1102 22 1.1197020959378392 1102 23 1.1440016756458888 1102 24 1.1338895343486257 1102 25 1.257965363781807 1102 26 1.2157329340567316 1102 27 1.3419437257117863 1102 28 1.3138613177537763 1102 29 1.2613846995761122 1102 30 1.2714117263182985 1102 31 1.326795661341997 1102 32 1.274015907586481 1102 33 1.3044850558169045 1102 34 1.2722753464219778 1102 35 1.302779474233752 1102 36 1.33486393357974 1102 37 1.2584004672134128 1102 38 1.2635739526358094 1102 39 1.2841139992639086 1102 40 1.3616225134084061 1102 41 1.2831416939597489 1102 42 1.3068117011755973 1102 43 1.3314490234846659 1102 44 1.2842210518640442 1102 45 1.3216717482390337 1102 46 1.3465309142915913 1102 47 1.4187709233096868 1102 48 1.369651056159924 1102 49 1.3626865336835365 1102 50 1.4066545060429476 1102 51 1.34026228830745 1102 52 1.3083159442017234 1102 53 1.3033493668501066 1102 54 1.3181415535486707 1102 55 1.2873767974944172 1102 56 1.3502863858806364 1102 57 1.356327462864101 1102 58 1.2473534597608449 1102 59 1.2121656220721349 1102 60 1.3200111587999004 1102 61 1.2947152715662398 1102 62 1.2974411434872906 1102 63 1.3275750643204915 1102 64 1.321236081506953 1102 65 1.288388671109466 1102 66 1.3401911483783522 1102 67 1.3424233825222487 1102 68 1.3561068772184797 1102 69 1.3052384232444965 1102 70 1.3310598624523564 1102 71 1.3717355417341146 1102 72 1.3351060168053408 1102 73 1.3669396904131688 1102 74 1.3677363628701755 1102 75 1.3557440025559693 1103 1 0.5229247297122477 1103 2 0.507098423782157 1103 3 0.5280192087697557 1103 4 0.51578156440452 1103 5 0.5350375176346276 1103 6 0.6151777168204973 1103 7 0.6753208939946873 1103 8 0.6358511425766551 1103 9 0.7668169220851837 1103 10 0.7890894786095757 1103 11 0.7802039085361585 1103 12 0.7704233957567581 1103 13 0.7468059718763556 1103 14 0.8504136587563096 1103 15 0.8171769263936142 1103 16 0.8942354709680842 1103 17 0.8753571079926061 1103 18 0.9126766387146503 1103 19 0.9238385266141478 1103 20 0.9370834746329422 1103 21 0.9282169134218847 1103 22 1.0195493310281094 1103 23 1.0002310558206986 1103 24 0.9666375230612019 1103 25 0.9670364826395641 1103 26 0.9968127419669699 1103 27 1.053356381104308 1103 28 1.0021807567586052 1103 29 1.0115252075733707 1103 30 1.0062374948211783 1103 31 1.0480498034829822 1103 32 1.0545540721821922 1103 33 1.047745373987702 1103 34 1.0674120785631587 1103 35 1.0370618608529654 1103 36 1.0641062082477433 1103 37 1.1615440294025063 1103 38 1.085876380847914 1103 39 1.1515756819902094 1103 40 1.129176846064965 1103 41 1.034820282469198 1103 42 1.0823634914118543 1103 43 1.113801503521941 1103 44 1.1719305454947175 1103 45 1.18480755482922 1103 46 1.0829557317655372 1103 47 1.15983617939618 1103 48 1.1397225208348871 1103 49 1.1556707196585947 1103 50 1.1186949034355962 1103 51 1.0732305142177552 1103 52 1.106366439203967 1103 53 1.1192008737628214 1103 54 1.173821150744324 1103 55 1.1664142292042143 1103 56 1.2016051775055416 1103 57 1.2025552103644372 1103 58 1.1893373678809454 1103 59 1.1449676328695944 1103 60 1.0884347365159925 1103 61 1.0983893265195661 1103 62 1.122944554085798 1103 63 1.0830880670791245 1103 64 1.1370029517126525 1103 65 1.0748864531693911 1103 66 1.1728991895135294 1103 67 1.1507134966071533 1103 68 1.1030109597265465 1103 69 1.056998368887342 1103 70 1.1176442963789839 1103 71 1.1086714590237428 1103 72 1.1229743445325795 1103 73 1.1083533715614848 1103 74 1.139713496892675 1103 75 1.1533720252559867 1104 1 0.45825129910313933 1104 2 0.4804303666456953 1104 3 0.44458388899296253 1104 4 0.5101925588720633 1104 5 0.49553398025759066 1104 6 0.5250773309141863 1104 7 0.47988593060282625 1104 8 0.5637020437108973 1104 9 0.6503073147301279 1104 10 0.6941677449893504 1104 11 0.6732013333493683 1104 12 0.6996918175726634 1104 13 0.6320869686009303 1104 14 0.7628301187739339 1104 15 0.7468986031220695 1104 16 0.757185181937416 1104 17 0.8090434538572353 1104 18 0.7165008482629531 1104 19 0.7484274980704129 1104 20 0.8342785735325933 1104 21 0.8542396625089665 1104 22 0.9039957817206243 1104 23 0.8166657408292863 1104 24 0.8198541404557531 1104 25 0.912236299526235 1104 26 0.9349945984343364 1104 27 0.9122371377030234 1104 28 0.8544805810907263 1104 29 0.8931173334740095 1104 30 0.9273823289548204 1104 31 0.8862826119762346 1104 32 0.9290247826354019 1104 33 0.879271190932414 1104 34 0.9634154162944455 1104 35 0.9765199519814658 1104 36 0.9475442601580539 1104 37 0.874388136241457 1104 38 0.8908570489277707 1104 39 0.8744627003068643 1104 40 0.9083396260138343 1104 41 0.8667089939161627 1104 42 0.8482289888561994 1104 43 0.8589886371202837 1104 44 0.951724640742853 1104 45 0.9374532431130902 1104 46 1.0054704440592346 1104 47 0.9762212595773221 1104 48 0.8956500901640041 1104 49 0.8766999060522522 1104 50 0.8145661779838065 1104 51 0.7832331267260884 1104 52 0.8157756051421003 1104 53 0.8542929627004057 1104 54 0.8791819853914902 1104 55 0.8893177007006088 1104 56 0.9486824455999496 1104 57 0.9439454331076575 1104 58 0.8978940628778957 1104 59 0.9402158677660566 1104 60 0.8379143952725201 1104 61 0.8955098464762727 1104 62 0.8317041590540057 1104 63 0.8245318758664553 1104 64 0.8971362330348214 1104 65 0.8299935145005435 1104 66 0.8625232181296809 1104 67 0.8838098261631373 1104 68 0.9482996539731445 1104 69 0.9060809927936475 1104 70 0.9297520404038906 1104 71 0.9538743589947849 1104 72 0.935034370562569 1104 73 0.9469132058704162 1104 74 1.0087907531377809 1104 75 1.0625367369440148 1105 1 0.4187069166294606 1105 2 0.48751435664385667 1105 3 0.469233424564905 1105 4 0.4961086474345251 1105 5 0.48161133913075815 1105 6 0.5773539690878948 1105 7 0.5995388078999753 1105 8 0.6387563875115845 1105 9 0.7942219613718606 1105 10 0.783062183288898 1105 11 0.8113305955709436 1105 12 0.783223442787488 1105 13 0.8103667765941367 1105 14 0.8723368450558411 1105 15 0.7964298049502361 1105 16 0.8236473747845992 1105 17 0.9007284609979891 1105 18 0.8418689199809748 1105 19 0.8883486345149834 1105 20 0.8701768083972468 1105 21 0.8517454534556848 1105 22 0.8882618617530227 1105 23 0.8757289040015763 1105 24 0.8273193560024623 1105 25 0.8706108035036237 1105 26 0.9153852628527943 1105 27 0.9543709223782244 1105 28 0.9073367306550679 1105 29 0.9376082266024355 1105 30 0.9719267070801294 1105 31 0.9601876987183218 1105 32 0.8919242224344117 1105 33 0.8814468453735316 1105 34 0.9313954778413276 1105 35 0.9118827897069721 1105 36 0.9585345935783067 1105 37 0.9063202375452306 1105 38 0.9100077579083674 1105 39 0.9571098663050179 1105 40 0.9557540213920319 1105 41 0.8926059148472163 1105 42 0.9242170553242772 1105 43 1.0018155018370152 1105 44 0.990403370716237 1105 45 1.015324517770722 1105 46 1.069871417167299 1105 47 1.0224303038757938 1105 48 0.966010980542805 1105 49 0.9601551267170052 1105 50 0.9366815936789123 1105 51 0.9493020127930478 1105 52 0.9123155896705839 1105 53 0.9185944486411728 1105 54 0.9938580221271351 1105 55 0.9316999586980259 1105 56 0.9188754126562415 1105 57 0.9653031410566726 1105 58 0.9201955853450059 1105 59 0.87991879919322 1105 60 0.8770539441519318 1105 61 0.9532178570938399 1105 62 0.9499479639621882 1105 63 0.9499461309371355 1105 64 0.9199754319136346 1105 65 0.9224231444061104 1105 66 0.8626756467471424 1105 67 0.8604422455214618 1105 68 0.959056588433441 1105 69 0.893846317875898 1105 70 0.9231807417867159 1105 71 0.911835350748671 1105 72 0.9145229648507467 1105 73 0.9341102890972763 1105 74 0.9684429361445979 1105 75 0.9265395910715846 1106 1 0.24828532221199495 1106 2 0.3296433806347707 1106 3 0.29776097790648137 1106 4 0.35891508732588306 1106 5 0.3336706483862599 1106 6 0.3616627644555308 1106 7 0.41816689112262395 1106 8 0.4504257206411637 1106 9 0.4808398778273997 1106 10 0.5245121307079401 1106 11 0.4938635182773581 1106 12 0.5433880137918621 1106 13 0.5281353891512666 1106 14 0.5890401738445163 1106 15 0.5586292561896613 1106 16 0.537845718073136 1106 17 0.6105623478419915 1106 18 0.5150325394805719 1106 19 0.5650057991478974 1106 20 0.5543346374381599 1106 21 0.5701995635686572 1106 22 0.663163462439492 1106 23 0.5716924545644915 1106 24 0.6484190300312065 1106 25 0.7125194155420544 1106 26 0.6887922592573261 1106 27 0.7194055761707574 1106 28 0.7367093649651792 1106 29 0.6909483234651717 1106 30 0.6183171938172052 1106 31 0.6693768378993212 1106 32 0.7427613834922155 1106 33 0.7207760833135879 1106 34 0.682879763929698 1106 35 0.7361970690550521 1106 36 0.692594378865067 1106 37 0.6721101412764838 1106 38 0.6605112578675119 1106 39 0.7804347494096433 1106 40 0.7580940691736373 1106 41 0.6543534570953398 1106 42 0.7220700832539606 1106 43 0.733155745943165 1106 44 0.8409469512799959 1106 45 0.8055809848379809 1106 46 0.7577599354997986 1106 47 0.8276120030814909 1106 48 0.7661947001623517 1106 49 0.825786311532859 1106 50 0.7839357271612002 1106 51 0.7697810614221368 1106 52 0.8624671416244993 1106 53 0.8268888888917232 1106 54 0.9220049108152963 1106 55 0.8572620701796936 1106 56 0.8620677962119387 1106 57 0.8280704345582492 1106 58 0.8126873635573872 1106 59 0.8256901036833852 1106 60 0.789108529348006 1106 61 0.8780248232130745 1106 62 0.9207513803439653 1106 63 0.8052728216777254 1106 64 0.7913985743390057 1106 65 0.8052750805593618 1106 66 0.8183077978478863 1106 67 0.857946358158209 1106 68 0.8391223683117914 1106 69 0.8488383923877549 1106 70 0.8125572272928565 1106 71 0.8246911297318498 1106 72 0.868075163337096 1106 73 0.858164619684942 1106 74 0.8988564461179678 1106 75 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-1.5847371523723695 2215 16 -1.55038204846646 2215 17 -1.601886137825126 2215 18 -1.5528117895444922 2215 19 -1.5999077853875576 2215 20 -1.5803267738105973 2215 21 -1.5293424360839722 2215 22 -1.5393371529032578 2215 23 -1.5106074375873035 2215 24 -1.6081776197802853 2215 25 -1.493396538115654 2215 26 -1.5580006360998695 2215 27 -1.5198789300954871 2215 28 -1.463300519833389 2215 29 -1.5569048850295175 2215 30 -1.4984742146079313 2215 31 -1.5137266855680238 2215 32 -1.5602945356838305 2215 33 -1.630631314025969 2215 34 -1.6926476371110368 2215 35 -1.8057405087227991 2215 36 -1.7530598831750481 2215 37 -1.6411621524896205 2215 38 -1.618761990399456 2215 39 -1.6102908523731045 2215 40 -1.6205325110777977 2215 41 -1.678830443544463 2215 42 -1.642450763070137 2215 43 -1.7472027099895158 2215 44 -1.644121035494031 2215 45 -1.6632802849626813 2215 46 -1.6320605894138964 2215 47 -1.6547025477772692 2215 48 -1.7217269324806281 2215 49 -1.743977788591728 2215 50 -1.7080516747753478 2215 51 -1.6833118204417445 2215 52 -1.664885247372652 2215 53 -1.6412441211824884 2215 54 -1.6858366830410745 2215 55 -1.6255487003754538 2215 56 -1.6818742474168076 2215 57 -1.7333853660002134 2215 58 -1.7188756126487519 2215 59 -1.6435946117923734 2215 60 -1.6324378743755332 2215 61 -1.7240124421967131 2215 62 -1.6815985760660368 2215 63 -1.6757914144892077 2215 64 -1.6470949655098615 2215 65 -1.6480875832927602 2215 66 -1.581439774725002 2215 67 -1.5801676559728435 2215 68 -1.546298859602036 2215 69 -1.4398014466922184 2215 70 -1.4043256997644882 2215 71 -1.4431169877939105 2215 72 -1.5292477411593204 2215 73 -1.4738582523910182 2215 74 -1.5111015617412917 2215 75 -1.5076490745509759 2216 1 -0.6965599191502925 2216 2 -0.5990181144987652 2216 3 -0.7891467008934079 2216 4 -0.6295178173332872 2216 5 -0.7638598356688888 2216 6 -0.8755576004996399 2216 7 -0.888116808317001 2216 8 -0.9154193515537159 2216 9 -1.326239269542583 2216 10 -1.6899827371510838 2216 11 -1.6171581215829463 2216 12 -1.4226145340050493 2216 13 -1.5074281746830565 2216 14 -1.5904141430758543 2216 15 -1.3985216286675097 2216 16 -1.481669564769092 2216 17 -1.8230811330412209 2216 18 -1.5015658365122704 2216 19 -1.792876997379814 2216 20 -1.761798659479311 2216 21 -1.8112556066295156 2216 22 -1.9039685896859595 2216 23 -1.9660626533940828 2216 24 -1.986788871903343 2216 25 -1.9833310643200193 2216 26 -2.074758559245957 2216 27 -2.0232143313539765 2216 28 -1.8451776198237582 2216 29 -1.857969180175317 2216 30 -1.8920781491111853 2216 31 -1.8563098548624595 2216 32 -1.7877912796580446 2216 33 -1.7602580996647177 2216 34 -1.8563223491091492 2216 35 -1.8172150518666044 2216 36 -1.9361280427981669 2216 37 -1.852514960322445 2216 38 -1.921371322659894 2216 39 -1.8340743543357192 2216 40 -1.8111304722251091 2216 41 -1.6960767818093174 2216 42 -1.6427308780458816 2216 43 -1.6708791703857102 2216 44 -1.8119784467667657 2216 45 -1.8618614610382949 2216 46 -1.6771515623693034 2216 47 -1.9211711076642928 2216 48 -1.8633730661581467 2216 49 -1.7743424679800697 2216 50 -1.7328556156691448 2216 51 -1.7578252054754557 2216 52 -1.7305455275483794 2216 53 -1.8547845193042107 2216 54 -1.886170678830517 2216 55 -1.8832019923592895 2216 56 -2.0328015509594195 2216 57 -1.9810766367008412 2216 58 -1.991329645755357 2216 59 -2.133924541487417 2216 60 -1.9972184762925167 2216 61 -1.9300622708672037 2216 62 -1.858164101166011 2216 63 -1.8161744711841088 2216 64 -1.8363057516634491 2216 65 -1.7729876073247972 2216 66 -1.8307149320808271 2216 67 -1.8994561659596378 2216 68 -1.9995561792470085 2216 69 -2.0835292204346523 2216 70 -1.864135754426119 2216 71 -1.8339583858103232 2216 72 -1.8957835704940642 2216 73 -1.872609520373416 2216 74 -1.9279764160414494 2216 75 -1.859808661217965 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 113616.0 3 45017.0 4 25861.0 5 16289.0 6 11873.0 7 10813.0 8 10319.0 9 10466.0 10 11444.0 11 12213.0 12 13017.0 13 13923.0 14 14530.0 15 15487.0 16 16591.0 17 17816.0 18 18827.0 19 20898.0 20 23545.0 21 27167.0 22 34166.0 23 40931.0 24 42903.0 25 45369.0 26 47001.0 27 49233.0 28 55974.0 29 67164.0 30 77509.0 31 88050.0 32 106412.0 33 132736.0 34 171218.0 35 231091.0 36 324098.0 37 504988.0 38 859106.0 39 1412690.0 40 386164.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.03389080778161 18.462109969926285 14.551902436700942 25.952096785591166 2 14.850162181397348 19.791905250010632 38.81071267340416 26.547219895187858 3 17.958473384533665 23.88633814886434 29.49245659575074 28.662731870851253 4 12.176737651715257 16.510909512131946 36.678651392164625 34.63370144398817 5 13.752096915776615 37.27347842793014 34.356032088668684 14.618392567624557 6 32.51348931483084 36.835437874002395 16.92938046260401 13.721692348562764 7 28.220044303724606 32.0014509050303 21.03232790453685 18.746176886708238 8 26.227617206874037 35.10165544966162 19.46416704420893 19.20656029925541 9 25.892870559944324 15.966899704394736 19.34449283703186 38.79573689862907 10 14.236265377627848 29.440870827048037 33.53434565338663 22.788518141937487 11 34.759530012560106 23.303069167970214 23.03200660819852 18.90539421127116 12 23.244997240920913 25.04078086819803 30.195631009002838 21.518590881878218 13 27.91222251311405 22.028886534422167 25.857296279571646 24.201594672892142 14 21.65284559620357 21.3783431927951 27.653609425584303 29.315201785417027 15 23.473383530056285 29.041684558996106 24.167726224240166 23.31720568670744 16 22.942054758863613 28.447326818527895 25.7268667625897 22.883751660018785 17 21.937657839614662 27.849853749598086 27.110446368089004 23.10204204269825 18 21.775820893018132 27.38434686857576 28.385438056409708 22.454394181996395 19 22.588960978534185 26.975415495239734 28.76318970961228 21.6724338166138 20 22.93135186975654 26.398874111669578 28.61514486799217 22.05462915058171 21 23.04176912710245 26.207257584120967 28.595890578010515 22.155082710766067 22 22.610698689913736 26.21631845684086 28.438554552525876 22.734428300719532 23 22.44235329463395 26.220586499565435 28.371162942232825 22.96589726356779 24 22.234734925152054 26.25488297826263 28.635314151437786 22.87506794514754 25 22.582214085549 26.243209792725537 28.593961797694938 22.580614324030527 26 22.45492378742432 26.329554119943584 28.63282936016403 22.58269273246806 27 22.418276486788923 26.586992724956122 28.340749513907653 22.6539812743473 28 22.300301970368853 26.54548825047442 28.467327196358795 22.686882582797935 29 22.448044415369893 26.567137497482427 28.4397009206884 22.545117166459278 30 22.623353937690517 26.47163619461888 28.413836543275977 22.49117332441462 31 22.592521199767127 26.209280292683996 28.41414582068423 22.78405268686465 32 22.39925383616546 26.513338400359583 28.363901963966885 22.72350579950807 33 22.42877465642731 26.28609064185332 28.36976700812588 22.915367693593485 34 22.593668409411876 26.452640464815296 28.43887837675174 22.514812749021086 35 22.784443928579094 26.41832301711004 28.242802215273212 22.554430839037657 36 22.690181529177462 26.424938593224468 28.21492829877432 22.66995157882375 37 22.729925777031458 26.22213907598959 28.308509110884994 22.739426036093963 38 22.566004934849953 26.366334874181614 28.311833827930222 22.75582636303821 39 22.724961037790564 26.694921949754992 28.060379378481308 22.519737633973136 40 22.81893138587023 26.258504463044662 28.354199241706716 22.568364909378396 41 22.63030709045291 26.40375469986143 28.177279953429114 22.78865825625655 42 22.770749250951926 26.405624624841963 28.025839159154405 22.797786965051706 43 22.601910746014987 26.229799865625065 28.18648853013955 22.981800858220396 44 22.657034753117188 26.6046041613222 27.924615905337774 22.813745180222845 45 22.837342297369897 26.45405170473144 27.792863493906477 22.91574250399219 46 22.868483572028687 26.431328440629688 27.568186619522557 23.132001367819072 47 22.771220160315426 26.585066058765154 27.516601255220902 23.127112525698514 48 22.650831470686576 26.77724591501961 27.619601819509043 22.95232079478477 49 22.975359118608626 26.52017774517989 27.380755456027966 23.123707680183518 50 22.94668429792562 26.69594719673321 27.429000794108887 22.92836771123228 51 22.79221430290811 26.93478848616951 27.114348280849075 23.158648930073312 52 22.907743007993595 26.80203122921281 27.166617834039553 23.12360792875404 53 22.877666406906055 26.784921140384842 27.269109716834926 23.068302735874173 54 22.827285754023105 26.794640568620316 27.40232446453867 22.975749212817913 55 22.826776256769485 26.69489516915742 27.282157777523324 23.19617079654977 56 22.803941784259198 27.00703555623617 27.20088644302058 22.988136216484058 57 22.866621033274466 26.74718899149358 27.10286025394612 23.28332972128583 58 22.66859546724063 26.66219578184111 27.42203163092495 23.24717711999332 59 22.760881176618845 26.841666234243032 27.266020125060813 23.1314324640773 60 22.759049164722498 26.872781111258494 27.39692652210891 22.971243201910095 61 22.96402311303438 26.6762158199677 27.341685038675827 23.018076028322092 62 22.73866704439608 26.879701375239446 27.264474197139638 23.117157383224836 63 22.589173333874356 26.6731485477023 27.471750365386132 23.26592775303721 64 22.727171637324453 26.635794978801968 27.396044595068076 23.240988788805506 65 22.654179373217417 26.712972455911334 27.287925383713286 23.34492278715797 66 22.57335669301785 26.84763268586947 27.27891541348983 23.300095207622853 67 22.676857627193375 26.686578019591007 27.229233596301317 23.4073307569143 68 22.713595544922338 26.677789793581585 27.288033514532867 23.32058114696321 69 22.76757184447307 26.755487287689235 27.12650198626198 23.350438881575712 70 22.614559383983295 26.476681360207653 27.32356776993407 23.585191485874983 71 22.661914571531128 26.589298812366373 27.20869032570189 23.540096290400612 72 22.507426765855392 26.76242172558493 27.201950478342834 23.52820103021685 73 22.64677687994691 26.489734465143844 27.270725073748846 23.592763581160405 74 22.61122550471381 26.725337122954716 27.002125418830815 23.661311953500658 75 22.56339812341984 26.720580487132136 27.12434894068082 23.59167244876721 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1022.0 1 711.5 2 513.5 3 684.0 4 742.0 5 602.0 6 555.0 7 1099.0 8 1550.0 9 2753.5 10 4707.0 11 5427.5 12 5398.0 13 5176.0 14 5271.5 15 6303.5 16 7018.0 17 7758.0 18 9465.0 19 11657.5 20 12883.0 21 14753.5 22 19231.0 23 25099.5 24 28361.0 25 33021.0 26 43205.5 27 54363.5 28 59997.0 29 67181.0 30 80563.5 31 94323.0 32 101884.0 33 110437.0 34 127778.0 35 144737.0 36 152908.0 37 159587.0 38 172342.0 39 185583.0 40 192748.0 41 200593.5 42 215698.5 43 229735.5 44 236513.0 45 242505.5 46 251244.5 47 257161.0 48 260331.0 49 262503.0 50 260466.0 51 251203.5 52 246150.0 53 239769.5 54 225532.0 55 207792.0 56 197909.0 57 187542.5 58 164035.0 59 137010.5 60 123127.0 61 109835.0 62 84937.5 63 63117.0 64 52902.0 65 44563.5 66 30155.0 67 20653.0 68 17221.0 69 14434.5 70 9719.0 71 6632.5 72 5475.0 73 4835.5 74 3658.0 75 2353.5 76 1587.0 77 1362.5 78 873.0 79 517.5 80 427.0 81 337.0 82 173.5 83 75.0 84 50.0 85 42.0 86 24.5 87 10.0 88 5.0 89 5.0 90 4.0 91 2.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07351973026510213 2 0.01507846948658104 3 0.00226274574442872 4 0.14085592259068783 5 0.0918167605088447 6 0.06604876802272108 7 0.028050244659383616 8 0.02459760675624669 9 0.04782976349430363 10 0.02840136037834669 11 0.014844392340605656 12 0.10223319350474933 13 0.08457987541243905 14 0.045528004892212356 15 0.018492094532055404 16 0.030683612551606693 17 0.02065730813232771 18 0.017711837378804118 19 0.012562140167345654 20 0.011840402300588217 21 0.02664578178353131 22 0.01603428449931386 23 0.013264371605271807 24 0.020618295274665147 25 0.014649328052292835 26 0.03197103685447131 27 0.028479386093671823 28 0.03589182904955901 29 0.04921471994132466 30 0.03333648687266105 31 0.019252845256475404 32 0.010982119432011805 33 0.013088813745790268 34 0.013537461608909755 35 0.0055593322169153895 36 0.008816905831739495 37 0.0066126793738046225 38 0.0023017586020912845 39 0.007685532959525136 40 0.010338407280579498 41 0.011040638718505651 42 0.0066711986602984675 43 0.010904093716686677 44 0.009753214415641035 45 0.0049351264943143635 46 0.0020676814561158994 47 0.004798581492495389 48 0.005793409362890775 49 0.0014629821623461552 50 2.7309000363794897E-4 51 0.0017360721659841042 52 0.0031405350418364135 53 0.0 54 1.9506428831282068E-5 55 3.5111571896307727E-4 56 0.007997635820825648 57 0.0029649771823548744 58 2.5358357480666693E-4 59 0.007197872238743084 60 0.0014629821623461552 61 0.0011118664433830778 62 0.022490912442468224 63 0.008407270826282573 64 0.011060145147336933 65 0.017126644513865655 66 0.017068125227371812 67 0.015527117349700527 68 0.016736515937240015 69 0.005910447935878467 70 0.013264371605271807 71 0.006924782235105135 72 0.015566130207363091 73 0.006495640800816929 74 0.0182775238149113 75 0.0012093985875394884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 5126515.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.47835885133663 #Duplication Level Percentage of deduplicated Percentage of total 1 73.23258132845693 41.36056007878285 2 13.498076581408316 15.246984259352041 3 5.456852637168232 9.245822443225501 4 2.7082873079343197 6.1183848980013975 5 1.5723362912445007 4.440148664594332 6 0.9681246056322402 3.2806853333823853 7 0.6013076562691371 2.377260871357715 8 0.4045499998076153 1.827865604995413 9 0.2897515489633746 1.4728222764075696 >10 1.206233305332884 11.340641484708385 >50 0.047185352921304005 1.8078164926472344 >100 0.014540348708387912 1.324623289816562 >500 6.92144727505579E-5 0.0285756658140485 >1k 1.0382167994897627E-4 0.12780863691455477 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 1.9506428831282068E-5 11 0.0 0.0 0.0 0.0 1.9506428831282068E-5 12 0.0 0.0 0.0 0.0 1.9506428831282068E-5 13 0.0 0.0 0.0 0.0 1.9506428831282068E-5 14 0.0 0.0 0.0 0.0 1.9506428831282068E-5 15 0.0 0.0 0.0 0.0 3.9012857662564136E-5 16 0.0 0.0 0.0 0.0 3.9012857662564136E-5 17 0.0 0.0 0.0 0.0 3.9012857662564136E-5 18 0.0 0.0 0.0 0.0 3.9012857662564136E-5 19 0.0 0.0 0.0 0.0 3.9012857662564136E-5 20 0.0 0.0 0.0 3.9012857662564136E-5 3.9012857662564136E-5 21 0.0 0.0 0.0 3.9012857662564136E-5 3.9012857662564136E-5 22 0.0 0.0 0.0 7.802571532512827E-5 5.851928649384621E-5 23 0.0 0.0 0.0 9.753214415641035E-5 5.851928649384621E-5 24 0.0 0.0 0.0 2.5358357480666693E-4 5.851928649384621E-5 25 0.0 0.0 0.0 4.096350054569235E-4 7.802571532512827E-5 26 0.0 0.0 0.0 7.217378667574366E-4 7.802571532512827E-5 27 0.0 0.0 0.0 9.558150127328215E-4 7.802571532512827E-5 28 0.0 0.0 0.0 0.0017750850236466684 7.802571532512827E-5 29 0.0 0.0 0.0 0.003121028613005131 7.802571532512827E-5 30 0.0 0.0 0.0 0.0049936457808082094 7.802571532512827E-5 31 0.0 0.0 0.0 0.010611497284217446 7.802571532512827E-5 32 0.0 0.0 0.0 0.017185163800359504 7.802571532512827E-5 33 0.0 0.0 0.0 0.024656126042740537 7.802571532512827E-5 34 0.0 0.0 0.0 0.035092065467476445 7.802571532512827E-5 35 0.0 0.0 0.0 0.046717897050920554 7.802571532512827E-5 36 0.0 0.0 0.0 0.06132821224555083 7.802571532512827E-5 37 0.0 1.9506428831282068E-5 0.0 0.08110773108047084 7.802571532512827E-5 38 0.0 1.9506428831282068E-5 0.0 0.1023697385065683 7.802571532512827E-5 39 0.0 1.9506428831282068E-5 0.0 0.12454854808773602 7.802571532512827E-5 40 0.0 1.9506428831282068E-5 0.0 0.14700044767254167 7.802571532512827E-5 41 0.0 1.9506428831282068E-5 0.0 0.16970593083215402 7.802571532512827E-5 42 0.0 1.9506428831282068E-5 0.0 0.1928795682837171 7.802571532512827E-5 43 0.0 1.9506428831282068E-5 0.0 0.2182379257643838 7.802571532512827E-5 44 0.0 1.9506428831282068E-5 0.0 0.24810226830507665 7.802571532512827E-5 45 0.0 1.9506428831282068E-5 0.0 0.2813607294624126 7.802571532512827E-5 46 0.0 1.9506428831282068E-5 0.0 0.31879356638964285 7.802571532512827E-5 47 0.0 1.9506428831282068E-5 0.0 0.3562654161745357 7.802571532512827E-5 48 0.0 1.9506428831282068E-5 0.0 0.39406887524956036 7.802571532512827E-5 49 0.0 1.9506428831282068E-5 0.0 0.43370593863472556 7.802571532512827E-5 50 0.0 1.9506428831282068E-5 0.0 0.47305040558742145 9.753214415641035E-5 51 0.0 1.9506428831282068E-5 0.0 0.512160795394142 9.753214415641035E-5 52 0.0 1.9506428831282068E-5 0.0 0.5528316995073651 9.753214415641035E-5 53 0.0 1.9506428831282068E-5 0.0 0.5956092979343667 9.753214415641035E-5 54 0.0 1.9506428831282068E-5 0.0 0.642795349277238 9.753214415641035E-5 55 0.0 3.9012857662564136E-5 0.0 0.6948580078279298 9.753214415641035E-5 56 0.0 3.9012857662564136E-5 0.0 0.7501782399934458 9.753214415641035E-5 57 0.0 3.9012857662564136E-5 0.0 0.8096728479288562 9.753214415641035E-5 58 0.0 3.9012857662564136E-5 0.0 0.8752144488019639 9.753214415641035E-5 59 0.0 3.9012857662564136E-5 0.0 0.9404634532426024 9.753214415641035E-5 60 0.0 3.9012857662564136E-5 0.0 1.0083848384331267 9.753214415641035E-5 61 0.0 3.9012857662564136E-5 0.0 1.0788030465140548 9.753214415641035E-5 62 0.0 3.9012857662564136E-5 0.0 1.1491627353084892 9.753214415641035E-5 63 0.0 3.9012857662564136E-5 0.0 1.2213560284130642 9.753214415641035E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12075 0.0 34.043827 1 GGTATCA 6280 0.0 33.665928 1 ATCAACG 16105 0.0 25.275736 3 TATCAAC 16275 0.0 25.072527 2 TCAACGC 16425 0.0 24.846228 4 CAACGCA 16685 0.0 24.317825 5 AACGCAG 17420 0.0 23.368338 6 ACGCAGA 20015 0.0 20.061579 7 CGCAGAG 20500 0.0 19.536997 8 AGAGTAC 19565 0.0 19.412401 11 GAGTACT 14465 0.0 19.334501 12 GTACATG 12475 0.0 19.22214 1 AGTACTT 15205 0.0 18.498575 13 GTACTTT 15485 0.0 18.17821 14 CAGAGTA 21500 0.0 17.938293 10 TACATGG 13050 0.0 17.515722 2 GCAGAGT 22470 0.0 17.394072 9 ACATGGG 13065 0.0 16.729992 3 TACTTTT 17320 0.0 16.11145 15 CGCGTAA 130 0.0025098796 15.935816 10 >>END_MODULE