FastQCFastQC Report
Mon 6 Feb 2017
SRR2077273_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077273_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4789613
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91950.035.291541
GGTATCA45450.035.0915531
ATCAACG116650.027.5635223
TCAACGC116550.027.5297014
CAACGCA118400.027.1284035
TATCAAC119000.027.1047552
AACGCAG123650.025.9534856
ACGCAGA142550.022.3941127
CGCAGAG147650.021.4591758
GAGTACT82800.020.94530912
AGAGTAC143050.020.77631611
GTACATG132850.020.138851
CAGAGTA156400.019.48747410
GTACTTT89650.019.15542414
TACATGG134850.019.109552
AGTACTT90850.019.05343613
GCAGAGT164750.018.8135079
GTATACG2501.0440999E-917.951381
GTGGTAT25400.017.9405081
ACATGGG143900.017.429193
Produced by FastQC (version 0.11.5)