##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077273_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4789613
Sequences flagged as poor quality	0
Sequence length	75
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.05977643705243	31.0	31.0	34.0	26.0	34.0
2	30.141133949653135	31.0	31.0	34.0	26.0	34.0
3	30.237946990706764	31.0	31.0	34.0	26.0	34.0
4	33.64243019216793	37.0	35.0	37.0	28.0	37.0
5	33.66868701082947	37.0	35.0	37.0	28.0	37.0
6	33.708917192265844	37.0	35.0	37.0	28.0	37.0
7	33.58934636263932	37.0	35.0	37.0	28.0	37.0
8	33.52296396389437	37.0	35.0	37.0	28.0	37.0
9	34.970855891697305	39.0	35.0	39.0	27.0	39.0
10	34.88167958455099	39.0	35.0	39.0	27.0	39.0
11	34.911297635111644	39.0	35.0	39.0	27.0	39.0
12	34.82434530723046	39.0	35.0	39.0	27.0	39.0
13	34.797648578288054	39.0	35.0	39.0	27.0	39.0
14	35.93680533270642	40.0	36.0	41.0	27.0	41.0
15	35.9507573993974	40.0	36.0	41.0	27.0	41.0
16	35.928570846955694	40.0	36.0	41.0	27.0	41.0
17	35.85648026260159	40.0	36.0	41.0	27.0	41.0
18	35.820877594912155	40.0	36.0	41.0	27.0	41.0
19	35.901396000052614	40.0	36.0	41.0	27.0	41.0
20	35.88637453589674	40.0	36.0	41.0	27.0	41.0
21	35.858311934596806	40.0	36.0	41.0	27.0	41.0
22	35.795155683768186	40.0	36.0	41.0	26.0	41.0
23	35.7501564322629	40.0	36.0	41.0	26.0	41.0
24	35.721401708238226	40.0	36.0	41.0	26.0	41.0
25	35.74546690933067	40.0	36.0	41.0	26.0	41.0
26	35.68555684979141	40.0	36.0	41.0	26.0	41.0
27	35.66845859989105	40.0	36.0	41.0	26.0	41.0
28	35.62965713513806	40.0	36.0	41.0	26.0	41.0
29	35.593653808773276	40.0	36.0	41.0	25.0	41.0
30	35.50700672476044	40.0	36.0	41.0	25.0	41.0
31	35.48805091350804	40.0	36.0	41.0	25.0	41.0
32	35.42280889917411	40.0	35.0	41.0	25.0	41.0
33	35.304134801705274	40.0	35.0	41.0	25.0	41.0
34	35.27141274253264	40.0	35.0	41.0	25.0	41.0
35	35.221744429038424	40.0	35.0	41.0	24.0	41.0
36	35.14918240784798	40.0	35.0	41.0	24.0	41.0
37	35.076411810307015	40.0	35.0	41.0	24.0	41.0
38	34.997941169777185	40.0	35.0	41.0	24.0	41.0
39	34.9494959196077	40.0	35.0	41.0	23.0	41.0
40	34.904985851675285	40.0	35.0	41.0	23.0	41.0
41	34.81278153370638	39.0	35.0	41.0	23.0	41.0
42	34.72567867174237	39.0	35.0	41.0	22.0	41.0
43	34.63662972352881	39.0	35.0	41.0	21.0	41.0
44	34.537237350909145	39.0	34.0	41.0	21.0	41.0
45	34.43705764954287	39.0	34.0	41.0	19.0	41.0
46	34.338382662649366	39.0	34.0	41.0	19.0	41.0
47	34.22034849162135	39.0	34.0	41.0	18.0	41.0
48	34.1115868860386	39.0	34.0	41.0	15.0	41.0
49	34.006313662502585	39.0	34.0	41.0	15.0	41.0
50	33.88104091082098	39.0	33.0	41.0	13.0	41.0
51	33.77585704732303	39.0	33.0	41.0	13.0	41.0
52	33.66515770689615	38.0	33.0	40.0	9.0	41.0
53	33.572580916245215	38.0	33.0	40.0	9.0	41.0
54	33.56754000792966	38.0	33.0	40.0	8.0	41.0
55	33.46882869242254	38.0	33.0	40.0	8.0	41.0
56	33.31824387481828	38.0	33.0	40.0	7.0	41.0
57	33.22313765224873	38.0	33.0	40.0	7.0	41.0
58	33.117907647235796	38.0	33.0	40.0	2.0	41.0
59	32.998428683068965	38.0	33.0	40.0	2.0	41.0
60	32.9648025842589	38.0	33.0	40.0	2.0	41.0
61	32.847254882596985	37.0	33.0	40.0	2.0	41.0
62	32.6907589402317	37.0	32.0	40.0	2.0	41.0
63	32.4951072247382	37.0	32.0	40.0	2.0	41.0
64	32.34372714455218	37.0	32.0	40.0	2.0	41.0
65	32.183023972918065	36.0	32.0	40.0	2.0	41.0
66	32.01014424338668	36.0	32.0	39.0	2.0	41.0
67	31.837658491406298	36.0	32.0	39.0	2.0	41.0
68	31.645523970308247	36.0	31.0	39.0	2.0	41.0
69	31.671386602633657	35.0	32.0	39.0	2.0	41.0
70	31.56875054414626	35.0	32.0	39.0	2.0	41.0
71	31.404128266730527	35.0	32.0	39.0	2.0	41.0
72	31.201074283037062	35.0	32.0	38.0	2.0	40.0
73	30.995774397639224	35.0	32.0	37.0	2.0	40.0
74	30.778887981137515	35.0	32.0	37.0	2.0	40.0
75	30.560040445856483	35.0	31.0	37.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5732029490018427
1101	2	0.6704133553273728
1101	3	0.6709782931931478
1101	4	0.6967138238490449
1101	5	0.7013840953255439
1101	6	0.6283032624053106
1101	7	0.6164689566210413
1101	8	0.7237096501156941
1101	9	0.7768447760343875
1101	10	0.9041090843777511
1101	11	0.7843210575972179
1101	12	0.8063331145064296
1101	13	0.7623057554642187
1101	14	0.9382289298261668
1101	15	0.9349478325884704
1101	16	0.9026365823074585
1101	17	0.8998939398993571
1101	18	0.9446067493956178
1101	19	0.8530324282271806
1101	20	0.8873323903028307
1101	21	0.8919133145601563
1101	22	0.9291409318556276
1101	23	0.9638761487167784
1101	24	0.9456518749700606
1101	25	0.8771116159488059
1101	26	0.834222578820409
1101	27	0.8938989348493749
1101	28	0.9246914407130333
1101	29	0.9641836362782996
1101	30	0.9329357284242263
1101	31	0.9214710368941539
1101	32	0.9689771196780228
1101	33	0.9453899889702981
1101	34	0.9052703611793333
1101	35	0.9817329225055218
1101	36	1.0543573636641028
1101	37	0.9832390330271963
1101	38	0.9683067456544521
1101	39	0.9224533401165829
1101	40	0.9287677357600259
1101	41	0.989930605985883
1101	42	0.8806149854039802
1101	43	0.8916631983396641
1101	44	0.9417608181548403
1101	45	0.9862331876490416
1101	46	0.9652240954959623
1101	47	0.9333777117302162
1101	48	0.9214238166696447
1101	49	0.8963283520194878
1101	50	0.8734677786987675
1101	51	0.9413053278705519
1101	52	0.9436879668540357
1101	53	0.9718951381994643
1101	54	0.9366851934731457
1101	55	0.8595714636156302
1101	56	0.8406204160759856
1101	57	0.8435512293387646
1101	58	0.7358204206389942
1101	59	0.7741779565578639
1101	60	0.7951491677168221
1101	61	0.7110377499427472
1101	62	0.6936029719022798
1101	63	0.7837340597846847
1101	64	0.6509145183909766
1101	65	0.6744231401264287
1101	66	0.6877270662688417
1101	67	0.6237082916920826
1101	68	0.6685309464286711
1101	69	0.6821886463975488
1101	70	0.7190970929018476
1101	71	0.6704289087194439
1101	72	0.6488655962294452
1101	73	0.6941281271666568
1101	74	0.6475172987403433
1101	75	0.657797531358213
1102	1	0.7014924427183757
1102	2	0.7384722248548208
1102	3	0.7445476119572021
1102	4	0.7151573564288753
1102	5	0.7087713837514045
1102	6	0.7577430749242495
1102	7	0.7125138490096958
1102	8	0.8533693135918909
1102	9	0.9232409691029488
1102	10	0.9477213750955187
1102	11	0.897172780038737
1102	12	0.8946187864455126
1102	13	0.9274603827364061
1102	14	1.0752777539383231
1102	15	0.967944671474875
1102	16	0.9723809758405864
1102	17	1.0740076274093084
1102	18	1.064746882881316
1102	19	1.0695105269900012
1102	20	1.0451984625146764
1102	21	1.0486105516059823
1102	22	1.0573328443359387
1102	23	1.1281537381397087
1102	24	1.1259351119661858
1102	25	1.1265156976466244
1102	26	1.1385964977733778
1102	27	1.0364338900405983
1102	28	1.0745345988837585
1102	29	1.0830283238183611
1102	30	1.1042428084068519
1102	31	1.1578012080166218
1102	32	1.1388865845353635
1102	33	1.1633153884340928
1102	34	1.1633284563113122
1102	35	1.2152067388037722
1102	36	1.1514059046816314
1102	37	1.1392662040214532
1102	38	1.1327027780832708
1102	39	1.143404930606387
1102	40	1.1652602542438828
1102	41	1.0785610526153846
1102	42	1.073958081112842
1102	43	1.1745733189231586
1102	44	1.2492495186468133
1102	45	1.195692926244071
1102	46	1.1549616848826787
1102	47	1.2294116547758591
1102	48	1.216914818786762
1102	49	1.1988019982963678
1102	50	1.2263776334695748
1102	51	1.2215159683770764
1102	52	1.2605664799225522
1102	53	1.2298118094609336
1102	54	1.2653407310097649
1102	55	1.2057758024687075
1102	56	1.2231219093178396
1102	57	1.1779462841035908
1102	58	1.106433729509881
1102	59	1.0886084623409786
1102	60	1.1076261354178598
1102	61	1.1024005987437135
1102	62	1.1427391794251207
1102	63	1.1037582355719664
1102	64	1.0759919003318785
1102	65	1.124051059971805
1102	66	1.162215899420275
1102	67	1.1669310598407137
1102	68	1.1607068569852537
1102	69	1.2056742876570752
1102	70	1.1470689322285281
1102	71	1.1623630984359679
1102	72	1.1391763123321148
1102	73	1.1513840275910852
1102	74	1.1647822030551822
1102	75	1.212379450795737
1103	1	0.6174083282821634
1103	2	0.6455639973038636
1103	3	0.624723177827974
1103	4	0.6532656524238121
1103	5	0.5865758658334315
1103	6	0.5319684813163406
1103	7	0.5558261984833734
1103	8	0.527932643321094
1103	9	0.6748642048758597
1103	10	0.7132426078513987
1103	11	0.7010811462771471
1103	12	0.7022868153725668
1103	13	0.6381510411038605
1103	14	0.7286870256880817
1103	15	0.7930321656597314
1103	16	0.8460762029667563
1103	17	0.7389211205649673
1103	18	0.7917237052707335
1103	19	0.8169318777471446
1103	20	0.7599860291269636
1103	21	0.7815942371812739
1103	22	0.7909293865592488
1103	23	0.7857578198236439
1103	24	0.8798556485340328
1103	25	0.7882694435059676
1103	26	0.8269350906470692
1103	27	0.9000228090121283
1103	28	0.8106173287443426
1103	29	0.7773683298850997
1103	30	0.7229460105570737
1103	31	0.772975767719629
1103	32	0.7404126578361456
1103	33	0.7791600396189011
1103	34	0.817308966582047
1103	35	0.8477430731132287
1103	36	0.802450493906953
1103	37	0.8580924135007066
1103	38	0.9314295685951777
1103	39	0.8647583116689717
1103	40	0.9091478891059381
1103	41	0.9373761544147143
1103	42	0.851982647777966
1103	43	0.8529257809385911
1103	44	0.7628385703999854
1103	45	0.7743944890364531
1103	46	0.7479530495969584
1103	47	0.7546387995769308
1103	48	0.7769562601358615
1103	49	0.7528550667988938
1103	50	0.7997122415066826
1103	51	0.8284093531599339
1103	52	0.8571210895839627
1103	53	0.7568720543951031
1103	54	0.7182791313064669
1103	55	0.7278047725694918
1103	56	0.7948731656204586
1103	57	0.7400152020572577
1103	58	0.7312854550231904
1103	59	0.7300241429652203
1103	60	0.7569460100038157
1103	61	0.7686831300977204
1103	62	0.6874890110797978
1103	63	0.7340826258832465
1103	64	0.7497140088044887
1103	65	0.7089855318344078
1103	66	0.6575447799311078
1103	67	0.6791949706104958
1103	68	0.7122902661923938
1103	69	0.7088696878155716
1103	70	0.7132863252682569
1103	71	0.6923134844758039
1103	72	0.7414016098036242
1103	73	0.7433471272949213
1103	74	0.7905629640731568
1103	75	0.796732220151128
1104	1	0.5303565262355541
1104	2	0.5152446057223443
1104	3	0.6118007279774851
1104	4	0.6280334772297991
1104	5	0.567271393705461
1104	6	0.5921371163262066
1104	7	0.5238880832336861
1104	8	0.5782561246252911
1104	9	0.6882505918160007
1104	10	0.7766027175122048
1104	11	0.7456999511371976
1104	12	0.7302849996407659
1104	13	0.6630250372426545
1104	14	0.8172470964447669
1104	15	0.7719760036628358
1104	16	0.7479372276392837
1104	17	0.8445495260760367
1104	18	0.9231049646843701
1104	19	0.8980236081254915
1104	20	0.8571289553552148
1104	21	0.8873380572310055
1104	22	0.8998116477386446
1104	23	0.9545867322934569
1104	24	0.9155031746960134
1104	25	0.7873150332530159
1104	26	0.8485906511750798
1104	27	0.8424523916587887
1104	28	0.796525089970352
1104	29	0.8304613352310142
1104	30	0.8570995750856625
1104	31	0.8573938161741168
1104	32	0.9478867071397801
1104	33	1.0237918150954428
1104	34	0.9400698646616661
1104	35	0.9318837095139401
1104	36	0.8757126920224181
1104	37	0.9101208471933191
1104	38	0.9775718221899652
1104	39	0.9939395104088646
1104	40	0.9367559506028798
1104	41	0.919364379196189
1104	42	0.9539052345963128
1104	43	0.9342094826212133
1104	44	0.9609044807537899
1104	45	0.9835189894185774
1104	46	0.9479025962936092
1104	47	0.9523325096313542
1104	48	1.0678788062423905
1104	49	0.9775801905431365
1104	50	0.89973813638845
1104	51	0.8968698352296158
1104	52	0.9620802607252585
1104	53	0.8869495312324318
1104	54	0.8578030254764286
1104	55	0.8725010766699555
1104	56	0.9371617942060482
1104	57	0.9260274003079161
1104	58	0.8385372734008811
1104	59	0.8617383972710329
1104	60	0.8818873853665821
1104	61	0.8943340267659394
1104	62	0.8443152364148645
1104	63	0.8091840790480802
1104	64	0.8580429136649812
1104	65	0.9354466632846936
1104	66	0.9196776560560025
1104	67	0.9235338063744507
1104	68	0.9126149101396983
1104	69	0.9292359814244406
1104	70	0.9161130467158856
1104	71	0.8978053238413253
1104	72	0.8552294303159407
1104	73	0.8815946247345927
1104	74	0.9085182062532589
1104	75	0.8433710111938808
1105	1	0.4719677893146965
1105	2	0.41344878945495367
1105	3	0.4191082016871981
1105	4	0.4344744707777579
1105	5	0.3366434037805135
1105	6	0.3837236861323703
1105	7	0.41976404675652645
1105	8	0.3553594831086073
1105	9	0.48837921671349704
1105	10	0.5552167276829039
1105	11	0.49681673885704924
1105	12	0.5021672462899716
1105	13	0.49293266117989987
1105	14	0.516089476106707
1105	15	0.5619540424115499
1105	16	0.46403244020316947
1105	17	0.5651121138846733
1105	18	0.553190461706464
1105	19	0.5105957093227786
1105	20	0.5330511463603216
1105	21	0.6204350566028936
1105	22	0.5781820570045397
1105	23	0.5243813232270611
1105	24	0.6181451810215464
1105	25	0.6037487719484176
1105	26	0.5732036499817141
1105	27	0.5706626396120029
1105	28	0.5642253540595519
1105	29	0.5916984384788151
1105	30	0.6117438343381423
1105	31	0.522332221724227
1105	32	0.5561150583826446
1105	33	0.6748557124309542
1105	34	0.6824684518182949
1105	35	0.7266229859829068
1105	36	0.6766644010897025
1105	37	0.6022300797476348
1105	38	0.6246141955622022
1105	39	0.6156111958160793
1105	40	0.6236976572399158
1105	41	0.5924816484788096
1105	42	0.5848076270448317
1105	43	0.6258289630801315
1105	44	0.6630243515896694
1105	45	0.6930828714785591
1105	46	0.6811747688876792
1105	47	0.6716881242107817
1105	48	0.6602415379514639
1105	49	0.6575067602617963
1105	50	0.6660574880355057
1105	51	0.7337902776436209
1105	52	0.6517374982025999
1105	53	0.5791955705395182
1105	54	0.5918363387153605
1105	55	0.47948285420493875
1105	56	0.5685422343591924
1105	57	0.5866471100307962
1105	58	0.5831024816468613
1105	59	0.5435145972786515
1105	60	0.5890945902155948
1105	61	0.6382893913799634
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2212	4	-0.33678163857916843
2212	5	-0.31331757612488076
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2212	34	-1.8429993442966577
2212	35	-1.274269583090657
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2212	51	-0.3651291790742093
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2212	54	-0.3679836629941349
2212	55	-0.3136507087560503
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2212	57	-0.3193305913032276
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2212	59	-0.258023274171407
2212	60	-0.27001314649792363
2212	61	-0.3955077342995281
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2212	64	-0.252122588946861
2212	65	-0.24147656498708514
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2212	69	-1.0142903919438062
2212	70	-0.6999559338225225
2212	71	-0.5345510437452674
2212	72	-0.4734379436748526
2212	73	-0.39338207929464275
2212	74	-0.4057890706592424
2212	75	-0.40604316530582807
2213	1	-0.3624614757195559
2213	2	-0.3260037151291755
2213	3	-0.31671086534326065
2213	4	-0.2714251477110352
2213	5	-0.3739953706720982
2213	6	-0.2738771656452812
2213	7	-0.20504463844234522
2213	8	-0.3627058730226125
2213	9	-0.4119727012907646
2213	10	-0.42061512143915536
2213	11	-0.40089920322960637
2213	12	-0.4090196024322168
2213	13	-0.33913591392057185
2213	14	-0.4980190218795997
2213	15	-0.4595245781452917
2213	16	-0.40943676744107904
2213	17	-0.42871698909141287
2213	18	-0.43836458806330114
2213	19	-0.40495849317770904
2213	20	-0.45334396533147725
2213	21	-0.45318177612880817
2213	22	-0.36815858747600316
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2213	24	-0.3942888330481509
2213	25	-0.5325367488924257
2213	26	-0.44730227719841764
2213	27	-0.42840950730106897
2213	28	-0.5670029891501827
2213	29	-0.47116890011501766
2213	30	-0.5758621634628369
2213	31	-0.4321952195416898
2213	32	-0.37506480235086315
2213	33	-0.4109369983190163
2213	34	-0.4467309987298762
2213	35	-0.5174871515091084
2213	36	-0.5862790929442951
2213	37	-0.5638922881856274
2213	38	-0.5612423699867719
2213	39	-0.47823594763656274
2213	40	-0.3657595174830064
2213	41	-0.331313513980966
2213	42	-0.4275075550389218
2213	43	-0.39747388152314755
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2213	45	-0.4056301565458611
2213	46	-0.36998591453399854
2213	47	-0.44732852788656174
2213	48	-0.45482407236749367
2213	49	-0.37911615506043006
2213	50	-0.4375015960384374
2213	51	-0.49279450743032527
2213	52	-0.4739042806190028
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2213	55	-0.4125382145391896
2213	56	-0.43899550879805815
2213	57	-0.41179541172726886
2213	58	-0.38946345332066556
2213	59	-0.41451694391689387
2213	60	-0.3260819485099091
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2213	62	-0.32746219183145087
2213	63	-0.30067099411182596
2213	64	-0.33629806015743213
2213	65	-0.40465213354895724
2213	66	-0.41216609118250247
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2213	70	-0.3274334674541599
2213	71	-0.24820721902786858
2213	72	-0.24490832043142774
2213	73	-0.2852648242242708
2213	74	-0.4434303971282141
2213	75	-0.4189146128369252
2214	1	-0.6244168310300573
2214	2	-0.5434636976791829
2214	3	-0.541320823108137
2214	4	-0.5040179344861215
2214	5	-0.5251679397899593
2214	6	-0.4914315569869032
2214	7	-0.4839344136156569
2214	8	-0.4859239957200643
2214	9	-0.5753490168937105
2214	10	-0.614386216961158
2214	11	-0.5688305415752808
2214	12	-0.652865569057532
2214	13	-0.7435079950523118
2214	14	-0.8132886076506338
2214	15	-0.8124881552077028
2214	16	-0.8272806833746458
2214	17	-0.7276535315887642
2214	18	-1.0610921492708343
2214	19	-0.8735392215423445
2214	20	-0.8890996367970061
2214	21	-0.7971514572723777
2214	22	-0.7349088618465132
2214	23	-0.7970856982927259
2214	24	-0.8257589287813687
2214	25	-0.7375255524738833
2214	26	-0.8834580337188882
2214	27	-0.9492336564059727
2214	28	-0.799181492484287
2214	29	-0.9647832847787257
2214	30	-0.8923205941179688
2214	31	-0.9329848818789301
2214	32	-0.9763878534096477
2214	33	-0.8901865325523204
2214	34	-0.8932244373510869
2214	35	-0.9553598813024138
2214	36	-0.9414596649880806
2214	37	-0.924412339771699
2214	38	-0.9281735327623863
2214	39	-0.813345449103025
2214	40	-0.8434980436802206
2214	41	-0.8414083085947865
2214	42	-0.882303423344041
2214	43	-0.8246032196014781
2214	44	-0.9537072379904998
2214	45	-0.9903857056248455
2214	46	-0.9746577218222257
2214	47	-0.9245914806744651
2214	48	-0.9427148797199578
2214	49	-0.9552942190020275
2214	50	-0.9937871409565417
2214	51	-0.9575480858184591
2214	52	-0.8926943473443245
2214	53	-0.7183363213049816
2214	54	-0.7912890138423307
2214	55	-0.8316528779650767
2214	56	-0.788108728533345
2214	57	-0.8043931951738728
2214	58	-0.7282710944568933
2214	59	-0.8328783676530449
2214	60	-0.8693075568672342
2214	61	-1.0085515953186395
2214	62	-0.8946838300557332
2214	63	-0.9067541467582068
2214	64	-0.8536820257411897
2214	65	-0.9409108791342824
2214	66	-0.8864901553388371
2214	67	-0.9848070187895956
2214	68	-0.8970535585508941
2214	69	-0.8970491581547186
2214	70	-0.9172443671925059
2214	71	-0.896826592788468
2214	72	-0.8734485977392836
2214	73	-0.8941746482922461
2214	74	-0.8721261185598124
2214	75	-0.8797541748458855
2215	1	-0.9682750797471655
2215	2	-1.0027855817287055
2215	3	-1.09937337092531
2215	4	-1.2265915817266588
2215	5	-1.0641540738969795
2215	6	-1.107334831544044
2215	7	-1.1393835101800391
2215	8	-1.1207174680876832
2215	9	-1.28206583113721
2215	10	-1.1902141292568302
2215	11	-1.195202899000222
2215	12	-1.3211092054536806
2215	13	-1.4047831022916242
2215	14	-1.5320377992705545
2215	15	-1.5846173442223161
2215	16	-1.5734288812969126
2215	17	-1.8460425484929175
2215	18	-1.7333717609334514
2215	19	-1.5274260755381803
2215	20	-1.4760246720573207
2215	21	-1.6395250758778346
2215	22	-1.575015103330486
2215	23	-1.6468607034540028
2215	24	-1.590468529489307
2215	25	-1.5184346317478798
2215	26	-1.5369618380840464
2215	27	-1.5718416463019906
2215	28	-1.647955560733557
2215	29	-1.6493298086371908
2215	30	-1.6183608301561065
2215	31	-1.514361666457397
2215	32	-1.6724149005101197
2215	33	-1.5189615210897642
2215	34	-1.5556358630013136
2215	35	-1.5175450394258192
2215	36	-1.5746880896136304
2215	37	-1.7301542972475943
2215	38	-1.592209042306294
2215	39	-1.7045901471457796
2215	40	-1.595330192552943
2215	41	-1.7321358505868432
2215	42	-1.647697008234175
2215	43	-1.6921179348483975
2215	44	-1.5758991218361729
2215	45	-1.6104100510244592
2215	46	-1.68744032532193
2215	47	-1.6374066624392682
2215	48	-1.7100474292304355
2215	49	-1.6660014403809171
2215	50	-1.712239988308859
2215	51	-1.6449630589771687
2215	52	-1.5020006188500261
2215	53	-1.5689222766268287
2215	54	-1.6067716335052573
2215	55	-1.673647064976148
2215	56	-1.5527067598402624
2215	57	-1.5630989913971085
2215	58	-1.560747917467836
2215	59	-1.5684003132984365
2215	60	-1.6537590761930403
2215	61	-1.7118690811748678
2215	62	-1.7964578265658737
2215	63	-1.5730710752296453
2215	64	-1.6210189742737633
2215	65	-1.6246594745751395
2215	66	-1.7048748066917092
2215	67	-1.665391091616872
2215	68	-1.698705668678116
2215	69	-1.7503517965144404
2215	70	-1.6924145077398265
2215	71	-1.7260465774757279
2215	72	-1.745550871432826
2215	73	-1.6488320053530785
2215	74	-1.638103578002461
2215	75	-1.8299756695931109
2216	1	-0.8893197860225008
2216	2	-0.8046719745678637
2216	3	-0.9922957118566949
2216	4	-0.8839630026317522
2216	5	-0.7913431090891692
2216	6	-0.8634281950908473
2216	7	-0.8578034421932088
2216	8	-0.8916111247953964
2216	9	-0.9670377462598623
2216	10	-1.019849249107871
2216	11	-0.9752757505157419
2216	12	-0.9273543364911063
2216	13	-1.0615610348729874
2216	14	-1.2601519841126816
2216	15	-1.1476019790622445
2216	16	-1.1410058196806645
2216	17	-1.198507580110899
2216	18	-1.3142274732613544
2216	19	-1.1766145113362896
2216	20	-1.1974152134647085
2216	21	-1.3152608876617933
2216	22	-1.1949977467857096
2216	23	-1.293218691987832
2216	24	-1.2576432288256214
2216	25	-1.1847464544896056
2216	26	-1.224686773032026
2216	27	-1.2265640745843882
2216	28	-1.1563886807217116
2216	29	-1.3392027182774129
2216	30	-1.3404295939039983
2216	31	-1.282816104142455
2216	32	-1.2498448307074668
2216	33	-1.1579639506781092
2216	34	-1.1586951350907384
2216	35	-1.2778263136999968
2216	36	-1.2418815568262858
2216	37	-1.2373146241155553
2216	38	-1.2502691599052653
2216	39	-1.2490553452072888
2216	40	-1.3077388326719444
2216	41	-1.1982903415366906
2216	42	-1.1361730784834663
2216	43	-1.2192169097036967
2216	44	-1.2256118324496654
2216	45	-1.3888266971470102
2216	46	-1.1733120065019662
2216	47	-1.1553109219783337
2216	48	-1.2031638608850272
2216	49	-1.1942515594072987
2216	50	-1.278237989553908
2216	51	-1.3509021781193908
2216	52	-1.2435471749550615
2216	53	-1.2701016617558594
2216	54	-1.2167461596549813
2216	55	-1.3066604885285784
2216	56	-1.2199161136861605
2216	57	-1.1794161523978062
2216	58	-1.1921664217374932
2216	59	-1.1160369481739565
2216	60	-1.2181722663838848
2216	61	-1.2562622640772787
2216	62	-1.2459471914916627
2216	63	-1.2251020085739661
2216	64	-1.2790336287218977
2216	65	-1.4197055256746687
2216	66	-1.3867249028719044
2216	67	-1.3455667875926842
2216	68	-1.2687250097785707
2216	69	-1.1783324269836015
2216	70	-1.1548017176183443
2216	71	-1.1517808252699915
2216	72	-1.1156230523031958
2216	73	-1.066493264669301
2216	74	-1.1990444404559426
2216	75	-1.1808950495935306
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	172190.0
3	26982.0
4	19090.0
5	13987.0
6	11695.0
7	10455.0
8	10432.0
9	10802.0
10	11433.0
11	12391.0
12	13161.0
13	13850.0
14	14470.0
15	14977.0
16	16049.0
17	16913.0
18	17776.0
19	19263.0
20	21202.0
21	24672.0
22	30052.0
23	36037.0
24	38770.0
25	41746.0
26	44976.0
27	48987.0
28	56963.0
29	69297.0
30	78265.0
31	88169.0
32	107141.0
33	133402.0
34	174680.0
35	233954.0
36	330604.0
37	516503.0
38	843739.0
39	1208185.0
40	236353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.792109653228465	17.47216048446562	13.431349067309553	24.304380794996366
2	15.872078437931178	19.29828155705147	39.359284191161095	25.470355813856255
3	19.427387686019244	23.659315094611003	28.80925522058061	28.104041998789143
4	13.165897404317223	15.782302484146182	36.642196505563135	34.40960360597346
5	14.644131860650052	36.716586255416075	33.80986226057762	14.829419623356252
6	33.364293461037065	35.57134710212368	16.944638859831105	14.119720577008144
7	29.205927593353447	31.58733788862516	20.63101509032748	18.575719427693905
8	26.74177141538678	34.56321121287816	19.381062969613673	19.313954402121393
9	26.463034008432135	16.219428605830082	19.007877139780717	38.30966024595707
10	15.025348388790686	28.745315376935515	32.89617152786023	23.333164706413566
11	35.149265168477775	23.091738997076398	22.816460744622862	18.94253508982296
12	23.828590461371256	24.664369926658907	29.4683298482182	22.038709763751637
13	28.150999108116558	21.85126980990231	25.586996276674164	24.41073480530697
14	22.245334940511878	21.243030197961932	26.573779888617004	29.937854972909182
15	23.909497183809485	28.7787112807781	23.463974966452017	23.847816568960393
16	23.60583680319022	28.219544191068575	24.927581504523253	23.247037501217953
17	22.556970842869937	27.862611857361113	26.30227431802654	23.278142981742402
18	22.60443796272332	27.41372916059528	27.144546329927504	22.837286546753894
19	23.466094396185905	27.010332909331158	27.322792257198802	22.200780437284138
20	23.652485517128323	26.467304235024606	27.29372597775327	22.586484270093795
21	23.847193334585267	26.212824172960836	27.29606699208564	22.643915500368262
22	23.671418286476857	26.09464611058405	27.13247331461291	23.10146228832618
23	23.311207833920093	26.23855425264898	26.999885597427177	23.450352316003748
24	23.03403245972333	26.288264743331975	27.21963166476061	23.45807113218409
25	23.385693515666325	26.160492440476453	27.201002818911746	23.25281122494548
26	23.132087514597476	26.498981104422576	27.218481996165337	23.150449384814607
27	23.230039768088854	26.477477682871402	27.00155554240155	23.290927006638192
28	23.214821690807746	26.451680644585757	27.057789040895898	23.275708623710596
29	23.276671329701077	26.553338476057885	27.06630014993106	23.10369004430998
30	23.337801403918114	26.49620932070617	27.083639646098696	23.082349629277026
31	23.420305205783514	26.233499429300032	26.96243085348144	23.38376451143502
32	23.190891770763404	26.404788345348113	27.037211315853867	23.367108568034617
33	23.22264928875852	26.3612606401759	26.988899244834062	23.427190826231516
34	23.52024765708581	26.37695239362381	26.900282375603485	23.202517573686894
35	23.457570521266565	26.390663667676904	26.989715568007423	23.162050243049112
36	23.480707129825518	26.44626975504311	26.859244651072505	23.213778464058862
37	23.518675390335943	26.133286525169602	26.975184908635164	23.37285317585929
38	23.304766596897302	26.32602062537199	26.987375615450514	23.381837162280192
39	23.443755866345207	26.514479102007897	26.860792151101993	23.180972880544896
40	23.622744200028993	26.290844877278918	27.01583471528746	23.070576207404624
41	23.34858021199758	26.351007607680643	26.99871057678469	23.301701603537087
42	23.493341950920772	26.348699178245504	26.890661150071182	23.26729772076254
43	23.319327862949883	26.23844642839584	27.025341442181983	23.416884266472294
44	23.37208854780693	26.575533133470163	26.77617723618187	23.276201082541036
45	23.45264731714531	26.4834624907365	26.680205377318178	23.383684814800016
46	23.5582816951213	26.33621000806613	26.53517448885755	23.57033380795502
47	23.47455279851855	26.511012574442926	26.599795990604523	23.414638636434002
48	23.36578910251232	26.68958269419994	26.593173825169934	23.351454378117804
49	23.583017387948562	26.43133932659893	26.50038131098988	23.485261974462627
50	23.533887372367175	26.57979072349072	26.49244744284445	23.393874461297656
51	23.413980171115895	26.759115514739396	26.277854676639013	23.549049637505696
52	23.546092582947775	26.690368970418714	26.24309976080775	23.520438685825763
53	23.473449545296198	26.702437605725304	26.422993272287705	23.401119576690792
54	23.456683460101697	26.6110014118535	26.511801392440255	23.420513735604544
55	23.38396924942334	26.504925171616385	26.483837914302892	23.62726766465738
56	23.382516491161994	26.869132625037274	26.34488891678829	23.403461967012447
57	23.552624174424615	26.60307174396792	26.28803985480793	23.55626422679953
58	23.421656442809	26.41737403624927	26.562450628864028	23.598518892077706
59	23.422106085301202	26.68837327667682	26.32962403950553	23.55989659851645
60	23.24636544344876	26.822552270098836	26.51904644505565	23.412035841396754
61	23.581365459090904	26.44151684009573	26.51643099746945	23.460686703343914
62	23.286576048658887	26.75089717018074	26.477063271662104	23.485463509498274
63	23.280062445562656	26.452180644178142	26.58958097712904	23.678175933130163
64	23.403459145690384	26.471869797960473	26.48706388674837	23.63760716960077
65	23.38332755218265	26.48344082366011	26.4813893350644	23.65184228909284
66	23.33529404502355	26.59750397426006	26.347968361604618	23.719233619111773
67	23.325989658856113	26.52609732329135	26.465462823118884	23.68245019473365
68	23.336308806395888	26.543814363960198	26.530318019900097	23.589558809743817
69	23.315549711840887	26.626094510832537	26.398509071613773	23.6598467057128
70	23.251621014322012	26.346855422639752	26.56077950982653	23.840744053211708
71	23.29926848457457	26.35970638946082	26.477092016940357	23.863933109024256
72	23.065690101142494	26.604735773659094	26.47186829254616	23.857705832652258
73	23.285503595233248	26.399998493573523	26.454522763045603	23.85997514814763
74	23.200963093347372	26.561391447274136	26.39614745923246	23.84149800014604
75	23.288054249513106	26.606215957497128	26.452099925118528	23.653629867871246
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2459.0
1	2207.0
2	2198.5
3	2339.5
4	2237.0
5	1919.5
6	1460.5
7	1430.0
8	1541.0
9	2046.5
10	3081.0
11	3789.0
12	3968.0
13	4063.0
14	4345.5
15	4904.5
16	5276.0
17	5687.0
18	6633.5
19	8168.5
20	9168.0
21	10490.5
22	14179.5
23	19145.5
24	21745.0
25	25281.0
26	34055.0
27	43276.5
28	47260.0
29	54112.0
30	66950.5
31	79692.5
32	86448.0
33	93175.0
34	107520.0
35	123366.5
36	131595.0
37	137471.0
38	149590.5
39	162230.5
40	168627.0
41	176254.5
42	189876.5
43	201119.0
44	206367.0
45	214031.0
46	224808.0
47	230701.5
48	233482.0
49	237428.5
50	240025.0
51	238021.0
52	237367.0
53	232941.5
54	223462.0
55	210879.5
56	203351.0
57	196184.5
58	176434.5
59	150221.0
60	136591.0
61	123661.5
62	97898.5
63	73814.5
64	62564.0
65	52980.5
66	36501.0
67	25554.0
68	21503.0
69	18281.5
70	12779.5
71	9010.0
72	7521.0
73	6692.5
74	5135.0
75	3451.5
76	2497.0
77	2047.5
78	1295.0
79	795.5
80	599.0
81	480.5
82	279.0
83	152.0
84	108.0
85	89.5
86	68.5
87	51.5
88	37.0
89	34.5
90	27.0
91	25.0
92	28.0
93	22.0
94	12.5
95	10.0
96	11.0
97	8.0
98	5.5
99	12.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18974393129465783
2	0.12971820479024088
3	0.130219289115843
4	0.15295599039003777
5	0.15287247633577075
6	0.12612710045675923
7	0.12566777315829064
8	0.10082234201385373
9	0.12046902328016898
10	0.12270302423181163
11	0.14230794847099337
12	0.14389471550206664
13	0.1586349460801948
14	0.16147442392527328
15	0.144291407259835
16	0.1460452023994423
17	0.13130497182131418
18	0.1304280742515105
19	0.17101590462527974
20	0.14754845537624856
21	0.1602425916248348
22	0.15270544822723672
23	0.17228949395285173
24	0.15663060877778642
25	0.18143428289508987
26	0.16629736055919342
27	0.1804738712710192
28	0.17997278694541707
29	0.18266611519552833
30	0.17287409233272083
31	0.18043211424388567
32	0.16709074407473004
33	0.18116286221872205
34	0.19740634577365646
35	0.1856517426355741
36	0.1865495187189445
37	0.2050270032255216
38	0.19997440294236718
39	0.1691368384042719
40	0.19930629050823107
41	0.19168563305636593
42	0.24609503941132616
43	0.20490173214412102
44	0.1880945287228843
45	0.1827705077633621
46	0.2164684286601026
47	0.19711404658372192
48	0.22980979882925823
49	0.23720079263188903
50	0.22481983408680406
51	0.23657443722488644
52	0.22156278597039053
53	0.21145758540408172
54	0.1956734291476159
55	0.19782391604499153
56	0.22001777596645072
57	0.19755249536862374
58	0.22461104895113654
59	0.236866736414821
60	0.2390798588528969
61	0.22569673165660775
62	0.24081277547893745
63	0.2574738293052069
64	0.23882931669009586
65	0.26294399985969635
66	0.22415172165266795
67	0.2464708526555277
68	0.220080411507151
69	0.2152365963596641
70	0.22580112422444149
71	0.21907824285594685
72	0.21744971879774003
73	0.21066420188854507
74	0.20907743485747177
75	0.23791066209315867
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	4789613.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.93881972428139
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5432827736793	39.66837871461336
2	12.870041725045134	13.883897209023779
3	5.198590708320759	8.41217541109313
4	2.7179193532115993	5.864054480720637
5	1.5436463322438274	4.163123061647399
6	1.018647277243635	3.29667791099247
7	0.6735055491236714	2.54296660782394
8	0.47729330802824216	2.0595710157873004
9	0.33374456879607495	1.6201609317224561
>10	1.5369832081832704	14.179120232338446
>50	0.06585537316249533	2.408371300931934
>100	0.019868083583353173	1.6777769055474578
>500	5.82880742385579E-4	0.19964330437798253
>1k	3.8858636334100694E-5	0.024082913379699818
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.087851356675372E-5
2	0.0	0.0	0.0	0.0	2.087851356675372E-5
3	0.0	0.0	0.0	0.0	2.087851356675372E-5
4	0.0	0.0	0.0	2.087851356675372E-5	2.087851356675372E-5
5	0.0	0.0	0.0	2.087851356675372E-5	4.175702713350744E-5
6	0.0	0.0	0.0	2.087851356675372E-5	6.263554070026118E-5
7	0.0	0.0	0.0	2.087851356675372E-5	6.263554070026118E-5
8	0.0	0.0	0.0	2.087851356675372E-5	6.263554070026118E-5
9	0.0	0.0	0.0	2.087851356675372E-5	6.263554070026118E-5
10	0.0	0.0	0.0	2.087851356675372E-5	6.263554070026118E-5
11	0.0	0.0	0.0	4.175702713350744E-5	6.263554070026118E-5
12	0.0	0.0	0.0	4.175702713350744E-5	1.2527108140052235E-4
13	0.0	0.0	0.0	4.175702713350744E-5	1.2527108140052235E-4
14	0.0	0.0	0.0	4.175702713350744E-5	1.2527108140052235E-4
15	0.0	0.0	0.0	4.175702713350744E-5	1.879066221007835E-4
16	0.0	0.0	0.0	4.175702713350744E-5	1.879066221007835E-4
17	0.0	0.0	0.0	4.175702713350744E-5	1.879066221007835E-4
18	0.0	0.0	0.0	4.175702713350744E-5	2.0878513566753722E-4
19	0.0	0.0	0.0	4.175702713350744E-5	2.0878513566753722E-4
20	0.0	0.0	0.0	4.175702713350744E-5	2.505421628010447E-4
21	0.0	0.0	0.0	1.0439256783376861E-4	2.505421628010447E-4
22	0.0	0.0	0.0	1.879066221007835E-4	2.505421628010447E-4
23	0.0	0.0	0.0	2.2966364923429096E-4	2.505421628010447E-4
24	0.0	0.0	0.0	4.1757027133507445E-4	2.505421628010447E-4
25	0.0	0.0	0.0	4.384487849018282E-4	2.505421628010447E-4
26	0.0	0.0	0.0	7.098694612696266E-4	2.714206763677984E-4
27	0.0	0.0	0.0	9.604116240706712E-4	2.714206763677984E-4
28	0.0	0.0	0.0	0.0017746736531740665	2.714206763677984E-4
29	0.0	0.0	0.0	0.002943870412912275	2.714206763677984E-4
30	0.0	0.0	0.0	0.004864693661053618	2.714206763677984E-4
31	0.0	0.0	0.0	0.009457966645739436	2.922991899345521E-4
32	0.0	0.0	0.0	0.014573202469594098	2.922991899345521E-4
33	0.0	0.0	0.0	0.021546626000889843	2.922991899345521E-4
34	0.0	0.0	0.0	0.030378237239626668	3.1317770350130586E-4
35	0.0	0.0	0.0	0.04156912051140666	3.1317770350130586E-4
36	0.0	2.087851356675372E-5	0.0	0.055265425411197104	3.1317770350130586E-4
37	0.0	2.087851356675372E-5	0.0	0.07338797518713934	3.1317770350130586E-4
38	0.0	2.087851356675372E-5	0.0	0.09272147874995329	3.1317770350130586E-4
39	0.0	2.087851356675372E-5	0.0	0.11328681461320571	3.1317770350130586E-4
40	0.0	2.087851356675372E-5	0.0	0.1336851223679241	3.1317770350130586E-4
41	0.0	2.087851356675372E-5	0.0	0.15429221525831002	3.1317770350130586E-4
42	0.0	2.087851356675372E-5	0.0	0.1759641123406004	3.549347306348133E-4
43	0.0	2.087851356675372E-5	0.0	0.20032933767300198	3.549347306348133E-4
44	0.0	2.087851356675372E-5	0.0	0.2287032376102203	3.549347306348133E-4
45	0.0	2.087851356675372E-5	0.0	0.25962431620258253	3.549347306348133E-4
46	0.0	2.087851356675372E-5	0.0	0.29522218183389765	3.549347306348133E-4
47	0.0	2.087851356675372E-5	0.0	0.3296717292190413	3.75813244201567E-4
48	0.0	2.087851356675372E-5	0.0	0.3657498006623917	3.75813244201567E-4
49	0.0	2.087851356675372E-5	0.0	0.4031223399468809	3.75813244201567E-4
50	0.0	2.087851356675372E-5	0.0	0.4407454213941711	3.9669175776832076E-4
51	0.0	2.087851356675372E-5	0.0	0.47834762432789457	3.9669175776832076E-4
52	0.0	2.087851356675372E-5	0.0	0.5154278644224491	3.9669175776832076E-4
53	0.0	2.087851356675372E-5	0.0	0.5553475823620823	3.9669175776832076E-4
54	0.0	2.087851356675372E-5	0.0	0.5986496194995296	3.9669175776832076E-4
55	0.0	2.087851356675372E-5	0.0	0.6480481825984689	3.9669175776832076E-4
56	0.0	2.087851356675372E-5	0.0	0.6994510829998165	3.9669175776832076E-4
57	0.0	2.087851356675372E-5	0.0	0.7563241539556537	3.9669175776832076E-4
58	0.0	2.087851356675372E-5	0.0	0.8173102920841413	3.9669175776832076E-4
59	0.0	2.087851356675372E-5	0.0	0.8800084683251027	3.9669175776832076E-4
60	0.0	2.087851356675372E-5	0.0	0.946109842277445	3.9669175776832076E-4
61	0.0	2.087851356675372E-5	0.0	1.0092047102761748	3.9669175776832076E-4
62	0.0	2.087851356675372E-5	0.0	1.0730720832768743	3.9669175776832076E-4
63	0.0	2.087851356675372E-5	0.0	1.14159536480296	3.9669175776832076E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9195	0.0	35.29154	1
GGTATCA	4545	0.0	35.091553	1
ATCAACG	11665	0.0	27.563522	3
TCAACGC	11655	0.0	27.529701	4
CAACGCA	11840	0.0	27.128403	5
TATCAAC	11900	0.0	27.104755	2
AACGCAG	12365	0.0	25.953485	6
ACGCAGA	14255	0.0	22.394112	7
CGCAGAG	14765	0.0	21.459175	8
GAGTACT	8280	0.0	20.945309	12
AGAGTAC	14305	0.0	20.776316	11
GTACATG	13285	0.0	20.13885	1
CAGAGTA	15640	0.0	19.487474	10
GTACTTT	8965	0.0	19.155424	14
TACATGG	13485	0.0	19.10955	2
AGTACTT	9085	0.0	19.053436	13
GCAGAGT	16475	0.0	18.813507	9
GTATACG	250	1.0440999E-9	17.95138	1
GTGGTAT	2540	0.0	17.940508	1
ACATGGG	14390	0.0	17.42919	3
>>END_MODULE