FastQCFastQC Report
Mon 6 Feb 2017
SRR2077273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4789613
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54350.038.6392861
GTATCAA112100.034.8748861
TATCAAC151350.025.6575072
ATCAACG151300.025.4640253
TCAACGC152950.025.167244
CAACGCA156200.024.6064855
GTGGTAT23550.024.2400231
AACGCAG162750.023.6336886
TTACGCG953.047766E-421.8103715
ACGCAGA189050.020.1823227
CGCAGAG193550.019.6782288
GAGTACT132950.019.53220612
TGGTATC28150.019.285832
AGAGTAC192350.019.1196211
AGTACTT141200.018.60175113
GTACATG127950.018.52211
CAGAGTA202950.018.32518810
GTACTTT145350.017.80124914
GCAGAGT212500.017.7771459
TACATGG129800.017.7159352
Produced by FastQC (version 0.11.5)