##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077272_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 255827 Sequences flagged as poor quality 0 Sequence length 75 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.699406239372703 31.0 29.0 34.0 16.0 34.0 2 28.581088000875592 31.0 28.0 34.0 16.0 34.0 3 28.72974314673588 31.0 28.0 34.0 18.0 34.0 4 31.86934529975335 35.0 32.0 37.0 18.0 37.0 5 31.759032471162154 35.0 32.0 37.0 17.0 37.0 6 31.214179895007174 37.0 32.0 37.0 8.0 37.0 7 31.099770548065685 37.0 32.0 37.0 8.0 37.0 8 30.855429645815335 37.0 32.0 37.0 8.0 37.0 9 31.861601785581662 39.0 32.0 39.0 7.0 39.0 10 32.17229612198869 39.0 32.0 39.0 7.0 39.0 11 31.85186473671661 39.0 32.0 39.0 7.0 39.0 12 31.951080222181396 39.0 32.0 39.0 7.0 39.0 13 31.542530694570942 38.0 31.0 39.0 2.0 39.0 14 32.42254726827114 39.0 31.0 41.0 2.0 41.0 15 32.4245408029645 38.0 31.0 41.0 2.0 41.0 16 32.43069339827305 38.0 31.0 41.0 2.0 41.0 17 32.84812392749788 39.0 32.0 41.0 2.0 41.0 18 32.83952436607551 39.0 32.0 41.0 2.0 41.0 19 32.944419470970615 39.0 32.0 41.0 2.0 41.0 20 33.00788423426769 39.0 32.0 41.0 2.0 41.0 21 32.98471623401752 39.0 32.0 41.0 2.0 41.0 22 33.019040210767436 39.0 32.0 41.0 2.0 41.0 23 32.94696025048177 39.0 32.0 41.0 2.0 41.0 24 32.92575060490097 39.0 32.0 41.0 2.0 41.0 25 32.881857661622895 39.0 32.0 41.0 2.0 41.0 26 32.69652147740465 38.0 32.0 41.0 2.0 41.0 27 32.53934494795311 38.0 31.0 41.0 2.0 41.0 28 32.468621373037244 38.0 31.0 41.0 2.0 41.0 29 32.31574462429689 38.0 31.0 41.0 2.0 41.0 30 31.981632118580134 37.0 31.0 40.0 2.0 41.0 31 31.547428535690134 37.0 30.0 40.0 2.0 41.0 32 31.108268478307608 35.0 30.0 40.0 2.0 41.0 33 30.83266817028695 35.0 30.0 40.0 2.0 41.0 34 30.610455503132975 35.0 30.0 40.0 2.0 41.0 35 30.416730837636372 35.0 30.0 40.0 2.0 41.0 36 30.23203571163325 35.0 30.0 40.0 2.0 41.0 37 30.02577523091777 35.0 30.0 40.0 2.0 41.0 38 29.915442857868793 35.0 29.0 40.0 2.0 41.0 39 29.732987526727047 35.0 28.0 40.0 2.0 41.0 40 29.554730345116035 35.0 27.0 40.0 2.0 41.0 41 29.374628166690773 35.0 26.0 40.0 2.0 41.0 42 29.108772725318282 35.0 25.0 40.0 2.0 41.0 43 28.85736454713537 35.0 25.0 40.0 2.0 41.0 44 28.457234771935724 35.0 24.0 40.0 2.0 41.0 45 28.03745108999441 35.0 22.0 40.0 2.0 41.0 46 27.413130748513645 35.0 15.0 40.0 2.0 41.0 47 26.81566058312844 35.0 7.0 39.0 2.0 41.0 48 26.32428555234592 35.0 2.0 39.0 2.0 41.0 49 26.051429286197312 35.0 2.0 39.0 2.0 41.0 50 25.962662267860704 35.0 2.0 39.0 2.0 41.0 51 25.90434942363394 35.0 2.0 39.0 2.0 41.0 52 25.86979091339069 35.0 2.0 39.0 2.0 41.0 53 25.823560452962354 35.0 2.0 39.0 2.0 41.0 54 25.782243469219434 35.0 2.0 39.0 2.0 41.0 55 25.743514171686336 35.0 2.0 39.0 2.0 41.0 56 25.61857817978556 35.0 2.0 39.0 2.0 41.0 57 25.532195585297877 35.0 2.0 39.0 2.0 41.0 58 25.445250110426187 35.0 2.0 39.0 2.0 41.0 59 25.369312074175127 35.0 2.0 39.0 2.0 41.0 60 25.2532766283465 34.0 2.0 39.0 2.0 41.0 61 25.150765947300325 34.0 2.0 39.0 2.0 41.0 62 24.995430505771477 34.0 2.0 38.0 2.0 41.0 63 24.933056323218423 34.0 2.0 38.0 2.0 41.0 64 24.806963299417184 34.0 2.0 38.0 2.0 41.0 65 24.67590989223186 34.0 2.0 38.0 2.0 40.0 66 24.501237164177354 34.0 2.0 37.0 2.0 40.0 67 24.33507800193099 34.0 2.0 37.0 2.0 40.0 68 24.168770301805516 33.0 2.0 37.0 2.0 40.0 69 24.030262638423622 33.0 2.0 37.0 2.0 40.0 70 23.849476403976123 33.0 2.0 36.0 2.0 39.0 71 23.658226066834228 33.0 2.0 36.0 2.0 39.0 72 23.514777564526028 33.0 2.0 36.0 2.0 39.0 73 23.345948629347177 33.0 2.0 35.0 2.0 39.0 74 23.154764743361724 33.0 2.0 35.0 2.0 39.0 75 22.993198528693217 33.0 2.0 35.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.1101266530515552 1101 2 0.9568669918378987 1101 3 1.3197068234858165 1101 4 1.3216842118814114 1101 5 1.2053774512105022 1101 6 1.1709478239268911 1101 7 1.1439405806840774 1101 8 1.3945870656060997 1101 9 1.1950688232748945 1101 10 1.6085295792999972 1101 11 1.5766966281034343 1101 12 1.09224908348844 1101 13 1.53858077626737 1101 14 1.336689494529388 1101 15 1.4533449533838834 1101 16 1.7174653307874337 1101 17 1.554479706517185 1101 18 2.0216140630751838 1101 19 1.7301237440260664 1101 20 1.7499385003664045 1101 21 1.7431128996178273 1101 22 1.5943721070571257 1101 23 1.8876686846161803 1101 24 1.6223012381215938 1101 25 1.7921657574334091 1101 26 1.732461505449976 1101 27 1.531479972480355 1101 28 2.0433237986435415 1101 29 2.014177374818466 1101 30 1.6585249605583883 1101 31 2.138093431581904 1101 32 1.710433634104124 1101 33 2.0108701060798175 1101 34 2.233748003107916 1101 35 1.9911361466236706 1101 36 1.9131953362145957 1101 37 1.9146134785674889 1101 38 2.16099298788842 1101 39 2.5555945236331326 1101 40 2.4596599415536247 1101 41 2.1981483636861157 1101 42 2.2391146417764496 1101 43 1.9580239097975785 1101 44 1.803258002568981 1101 45 1.0893839817545583 1101 46 1.5476322295651208 1101 47 1.5837091375275243 1101 48 1.5200830389061935 1101 49 1.293510600247238 1101 50 1.5512283223984618 1101 51 1.4722430913320146 1101 52 1.7340450431667271 1101 53 1.7094552446220135 1101 54 1.8233524969855104 1101 55 1.7647011217183746 1101 56 1.9221414908113026 1101 57 1.7249504830649265 1101 58 1.8058519726500357 1101 59 1.8877009477417381 1101 60 1.7457398429138742 1101 61 1.9076944461436867 1101 62 2.3301070984987007 1101 63 2.204558195251259 1101 64 2.07340168634331 1101 65 2.1521532741398985 1101 66 2.044349649132073 1101 67 1.899832831079518 1101 68 2.191783357556325 1101 69 2.306879294819062 1101 70 2.0774169950548327 1101 71 2.0163760397379384 1101 72 2.0661790047148614 1101 73 2.1239914252208933 1101 74 2.261153069367321 1101 75 2.256425476684811 1102 1 0.9749592884546452 1102 2 1.4540059400431566 1102 3 1.1624645261398001 1102 4 1.4969617662598793 1102 5 1.4291311141887704 1102 6 1.3498120926716304 1102 7 1.5310931177298421 1102 8 1.231447026595962 1102 9 1.5744747968182509 1102 10 1.8505126256037414 1102 11 2.1061622261510777 1102 12 1.9750707553780273 1102 13 1.6138370691947053 1102 14 1.877488370445711 1102 15 1.867897030073017 1102 16 2.052092839407564 1102 17 2.430534690478659 1102 18 1.7331978563029509 1102 19 1.7409393517800282 1102 20 1.6485953313008892 1102 21 1.616246745074804 1102 22 1.7157102843036753 1102 23 1.9114075570197429 1102 24 1.9277543381336102 1102 25 1.7891799101645844 1102 26 1.758520649260582 1102 27 2.076031956728393 1102 28 2.0597505806766847 1102 29 2.0960062293808903 1102 30 2.1626524553123545 1102 31 2.344311019424051 1102 32 2.325573636137829 1102 33 2.2984173665880583 1102 34 2.0858330119360424 1102 35 2.5203059331771875 1102 36 2.1136908204672515 1102 37 2.2477157091710325 1102 38 2.1288419761206683 1102 39 2.1742103107228132 1102 40 2.0717031670082022 1102 41 1.8503387553590223 1102 42 2.336233252941483 1102 43 2.3506119830479015 1102 44 2.4015552376559235 1102 45 2.6069431671636565 1102 46 2.2506190012448677 1102 47 2.258861896431913 1102 48 2.2430740628761683 1102 49 2.1164304445764373 1102 50 2.1124566796278152 1102 51 1.8611889855609185 1102 52 2.1166402343811264 1102 53 2.112812705080337 1102 54 1.9767215125803226 1102 55 2.343428577647085 1102 56 2.4575510510690286 1102 57 2.35259776483462 1102 58 2.105906512894265 1102 59 2.276776259499318 1102 60 2.200799606079798 1102 61 2.305939914219156 1102 62 2.077946287975209 1102 63 2.221807703002444 1102 64 2.356040324870907 1102 65 2.1011073031848895 1102 66 2.1474122225071923 1102 67 2.15092132493681 1102 68 2.4026784646493304 1102 69 2.321780798835629 1102 70 2.366739124527829 1102 71 2.293939662319449 1102 72 2.4839387872934147 1102 73 2.5326827366826627 1102 74 2.6481761888844098 1102 75 2.9186457268304054 1103 1 0.35709894747172655 1103 2 0.30544484580755693 1103 3 0.24521697113830854 1103 4 0.2557014287886332 1103 5 0.4459256640807894 1103 6 0.2766640583275226 1103 7 0.12341485670759766 1103 8 -0.23089911847102584 1103 9 0.19703199310291097 1103 10 0.7374720482855963 1103 11 0.014068745561822027 1103 12 0.12270125772207052 1103 13 -0.037945979986201195 1103 14 0.2546326824222547 1103 15 0.319093630778859 1103 16 0.11208089973801094 1103 17 0.26341443342459314 1103 18 0.4853092463965396 1103 19 0.19979138766927207 1103 20 0.3265666409772905 1103 21 0.3624146378987163 1103 22 0.6330456309930526 1103 23 0.43796454260434814 1103 24 0.4542059736927513 1103 25 0.44044498838697876 1103 26 0.413358849179815 1103 27 0.48779253123720423 1103 28 0.6345052029090539 1103 29 0.4196502867615379 1103 30 0.5403937507665049 1103 31 0.6546282985840541 1103 32 0.7052814654005566 1103 33 0.7973638663334661 1103 34 0.7399600991834205 1103 35 0.5258280303450285 1103 36 0.7304362070140549 1103 37 0.9180052461576729 1103 38 0.8824809415834167 1103 39 0.8425859889136014 1103 40 0.6581428628977974 1103 41 0.45561854746830477 1103 42 0.505829235593847 1103 43 0.5830492558869089 1103 44 0.6881775402563122 1103 45 0.5274565729543994 1103 46 0.08543016132423276 1103 47 0.2671318734304897 1103 48 -0.11693331162561549 1103 49 0.4261858569339978 1103 50 0.3513905373701647 1103 51 0.544963325806382 1103 52 -0.18831297264923208 1103 53 -0.1949761734686426 1103 54 -0.14712622151621346 1103 55 0.005553487660606038 1103 56 0.18478227681657344 1103 57 0.039076089066881536 1103 58 -0.18896820836712536 1103 59 -0.015403256944065191 1103 60 0.03938580873812825 1103 61 -0.08924558961734164 1103 62 -0.1356157719689577 1103 63 -0.042331148331111734 1103 64 0.029806412697713114 1103 65 0.29075490734972576 1103 66 0.20733882865611974 1103 67 0.19652032762323302 1103 68 0.10787397673824728 1103 69 0.15180731192537067 1103 70 0.23265487962413545 1103 71 0.11921664509471697 1103 72 0.10488939568980982 1103 73 -0.07970818214514352 1103 74 0.2637890626575228 1103 75 0.25288624093852974 1104 1 0.5448492729979257 1104 2 0.5829744728122215 1104 3 0.45591071279412176 1104 4 0.29358608537909703 1104 5 0.5959396740690863 1104 6 0.0658879049758383 1104 7 0.5074286359431497 1104 8 0.15622158169874467 1104 9 0.6589742719511236 1104 10 0.645583007392915 1104 11 0.43539029951675445 1104 12 0.863038486381555 1104 13 0.3947319056593308 1104 14 0.6531014731130256 1104 15 0.592403778955088 1104 16 0.8768037492048109 1104 17 0.606840452445212 1104 18 0.6209424154558931 1104 19 0.9109219113131886 1104 20 0.7444021801970138 1104 21 0.9435571936502285 1104 22 0.5717148469281739 1104 23 0.795746679045152 1104 24 1.0355380763447641 1104 25 0.8722081042083758 1104 26 1.0046957317101857 1104 27 0.8193512660528484 1104 28 0.7195705553838749 1104 29 0.7801767326747466 1104 30 0.5885139399837556 1104 31 0.5240304841422336 1104 32 0.5748979213679846 1104 33 0.6414986259385493 1104 34 0.6766102285697855 1104 35 0.4731257508375215 1104 36 0.42878467662886166 1104 37 0.35154021344022723 1104 38 0.5273359794103847 1104 39 0.3971672550868952 1104 40 0.69779112977983 1104 41 0.6883903824471247 1104 42 0.8685890384650712 1104 43 0.878198503468127 1104 44 0.6726511767548047 1104 45 0.5789144358543012 1104 46 0.5198551749221636 1104 47 0.4078745676336517 1104 48 0.6915007013295025 1104 49 0.6995536932973323 1104 50 0.49048499765596887 1104 51 0.4532391083054357 1104 52 0.5382779851394979 1104 53 0.6289182736312924 1104 54 0.8961316776448349 1104 55 0.8435980229410518 1104 56 0.9775827910655543 1104 57 1.092577762024309 1104 58 1.0343510050956475 1104 59 1.0549186431399953 1104 60 0.810067023816174 1104 61 0.9226546592659801 1104 62 0.9970340936999804 1104 63 1.0041572893079582 1104 64 0.668365196960373 1104 65 0.9763707699529469 1104 66 0.800933791653268 1104 67 0.936643285873469 1104 68 1.193301940674889 1104 69 1.291355036538448 1104 70 1.2607490267454935 1104 71 1.2654149935643346 1104 72 1.1368321690082013 1104 73 1.0431594152242774 1104 74 1.1654389448087947 1104 75 1.0757835065184587 1105 1 0.04345651533775907 1105 2 0.059297592589381054 1105 3 0.19407227268270333 1105 4 0.27130196281642327 1105 5 0.32295917267632746 1105 6 0.4001497434159518 1105 7 0.45728936017713195 1105 8 0.6743274312809149 1105 9 0.7731804000848257 1105 10 0.8043573806519717 1105 11 0.69722873962818 1105 12 0.5176345866601082 1105 13 0.8877681173584939 1105 14 0.9595081583497986 1105 15 0.7790333054174852 1105 16 1.0188650305418534 1105 17 1.1973697003560773 1105 18 1.2421123318904321 1105 19 1.1783313820652737 1105 20 1.2847921523418648 1105 21 1.319601761895349 1105 22 1.3572301672624363 1105 23 1.2219305230562938 1105 24 1.567571502528608 1105 25 1.462737352119241 1105 26 1.38631133059598 1105 27 1.4600197897297278 1105 28 1.3518825330997544 1105 29 1.3790625265466119 1105 30 1.2988203466689967 1105 31 1.4014678100475244 1105 32 1.2044243837635271 1105 33 1.3573760632644571 1105 34 1.7796742954221507 1105 35 1.8185296505589683 1105 36 1.9413194955703652 1105 37 2.287473361072543 1105 38 2.1930741409702748 1105 39 2.1798970601008207 1105 40 1.8760641102812237 1105 41 1.6438221373217772 1105 42 1.564967868548635 1105 43 1.5300090884263433 1105 44 1.0347681683982586 1105 45 0.7794715389183686 1105 46 0.9961782946993196 1105 47 1.2128884952102545 1105 48 1.4853725676247684 1105 49 1.1535926905118181 1105 50 1.4013485074052738 1105 51 1.5925148743221484 1105 52 1.1371637790113631 1105 53 1.1693082457761363 1105 54 1.3223155216559768 1105 55 1.336634234640215 1105 56 1.0973599498399302 1105 57 1.3599872327763975 1105 58 1.2321225928393815 1105 59 1.5618824314408322 1105 60 1.3226018427576776 1105 61 1.5243259684581787 1105 62 1.6293460714158563 1105 63 1.5724024146561462 1105 64 1.5068053083809865 1105 65 1.4304097671674292 1105 66 1.6950842094805658 1105 67 1.6413786619180364 1105 68 1.9119648933211266 1105 69 1.9013828916916502 1105 70 1.7816403916480006 1105 71 1.7166684754584836 1105 72 1.7106483249970559 1105 73 1.636474178455476 1105 74 1.6807592790706352 1105 75 1.80837403576637 1106 1 -0.08101404596265738 1106 2 0.0047396500549439224 1106 3 0.28073614111480083 1106 4 0.04685811193688494 1106 5 0.36322322426559595 1106 6 0.028516210390424845 1106 7 -0.09429609409081152 1106 8 0.6570801309413135 1106 9 0.5440126204839615 1106 10 0.6400577660451816 1106 11 0.7374698965709783 1106 12 0.7234956161560433 1106 13 0.47281028166574046 1106 14 0.5056904760511713 1106 15 0.8024850549500542 1106 16 0.6458654502770429 1106 17 0.32470972401387854 1106 18 0.4208203106747277 1106 19 0.7178589891931395 1106 20 0.7535829334308843 1106 21 0.45387123188428546 1106 22 0.5175232187372387 1106 23 0.605476903794262 1106 24 0.35059893794662855 1106 25 0.5962284041282473 1106 26 0.26917468771430464 1106 27 0.4867225790608245 1106 28 0.4117444020660628 1106 29 0.2767387199300586 1106 30 0.34605963388233363 1106 31 0.36579795084960054 1106 32 0.5490117077609327 1106 33 0.42178633532916976 1106 34 0.5177748029201474 1106 35 0.7730761457701725 1106 36 0.6284259938341883 1106 37 0.7727024983259447 1106 38 0.7519629874616669 1106 39 0.6769703611272675 1106 40 1.2390053091975446 1106 41 1.0458673924062936 1106 42 0.9555658003989045 1106 43 0.9692047231804146 1106 44 0.8744140645328784 1106 45 0.4692414477213589 1106 46 0.6469280943711659 1106 47 -0.16232423421318387 1106 48 0.01811572784913551 1106 49 0.11238995841733868 1106 50 0.1351142951718991 1106 51 0.015272152911837367 1106 52 -0.20137935831835918 1106 53 -0.4376364636526411 1106 54 -0.4481961736925939 1106 55 -0.5131463583311273 1106 56 -0.19034944078089922 1106 57 -0.1300574117842146 1106 58 -0.47681776660656894 1106 59 -0.27168112028198976 1106 60 -0.3433652652668968 1106 61 -0.42566449210579194 1106 62 -0.508769699179787 1106 63 -0.5913706230818612 1106 64 -0.4671863732307955 1106 65 -0.3678655027986082 1106 66 -0.2220524334011067 1106 67 -0.4968897396540619 1106 68 -0.41110146299588735 1106 69 -0.5511755850512046 1106 70 -0.5920918173038068 1106 71 -0.5542209815568171 1106 72 -0.5662624240394578 1106 73 -0.34585878601966513 1106 74 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-2.1952185431799904 2214 23 -2.224857854661952 2214 24 -2.3226756454230646 2214 25 -2.354985858734217 2214 26 -2.2119002621155524 2214 27 -2.3051571289344075 2214 28 -2.39930175446478 2214 29 -2.3106038621599296 2214 30 -2.241469086706328 2214 31 -2.1973233591000856 2214 32 -2.042309584738266 2214 33 -2.1447145517371133 2214 34 -2.13343655152827 2214 35 -1.961420553179888 2214 36 -2.090912051129891 2214 37 -1.933274292530001 2214 38 -2.308915935261769 2214 39 -2.1865726688584814 2214 40 -2.4929959705046336 2214 41 -2.0555373715759053 2214 42 -2.321216330305223 2214 43 -2.3339974406796316 2214 44 -2.0491935322445975 2214 45 -2.013241174504742 2214 46 -2.254121843097934 2214 47 -2.2664803578789474 2214 48 -1.8661701110828943 2214 49 -1.9930342337706612 2214 50 -1.6416998280866864 2214 51 -1.9381583214504907 2214 52 -1.8216626378460319 2214 53 -1.790316567192182 2214 54 -1.7118380897557621 2214 55 -1.8413666997789342 2214 56 -2.067381695092621 2214 57 -2.078450513026109 2214 58 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-0.9345622295903269 2215 20 -0.6621225742076504 2215 21 -0.7674828466996217 2215 22 -0.8710330696668436 2215 23 -1.0485453071533684 2215 24 -1.197436464274471 2215 25 -1.1223183008720383 2215 26 -1.1818879426481743 2215 27 -1.2036263695834073 2215 28 -1.220512207663912 2215 29 -1.1501624582648802 2215 30 -1.101118899293155 2215 31 -0.850753151241836 2215 32 -0.899023694460162 2215 33 -1.059723332634075 2215 34 -1.0517217865155573 2215 35 -1.1008795450804953 2215 36 -1.086862353874249 2215 37 -1.0565607070407594 2215 38 -1.0774016892393874 2215 39 -1.0279791850703752 2215 40 -1.1067413507043682 2215 41 -0.8655030343370385 2215 42 -0.7580200838110294 2215 43 -0.7316803323469543 2215 44 -0.5843040142765226 2215 45 -0.6916244409984103 2215 46 -0.6181883157985162 2215 47 -0.6228406105590878 2215 48 -0.5114349796315558 2215 49 -0.5895323596389197 2215 50 -0.49826977382860704 2215 51 -0.6197918304017662 2215 52 -0.5336754291228019 2215 53 -0.47126857369266517 2215 54 -0.4126191964108976 2215 55 -0.541479409799642 2215 56 -0.6251259219805156 2215 57 -0.5909796734675119 2215 58 -0.8847945409455953 2215 59 -0.8194064712036884 2215 60 -0.7006852945808362 2215 61 -0.787336514203993 2215 62 -0.7150834710947755 2215 63 -0.680580837862852 2215 64 -0.6682236357596878 2215 65 -0.5222823244909236 2215 66 -0.5953372859858064 2215 67 -0.63208901808013 2215 68 -0.5169586139063114 2215 69 -0.6395458426756484 2215 70 -0.4125833895457589 2215 71 -0.6760204065525883 2215 72 -0.43516709735813563 2215 73 -0.2517441234118536 2215 74 -0.41253255810854483 2215 75 -0.4249329326071418 2216 1 -1.0924482086688343 2216 2 -1.224950034037871 2216 3 -1.371290697017713 2216 4 -1.4673996459607928 2216 5 -1.6596171447587231 2216 6 -1.9599378013512592 2216 7 -1.6906897200343387 2216 8 -2.2231826591062145 2216 9 -2.837927552845649 2216 10 -2.938835160988642 2216 11 -3.5867403354670415 2216 12 -3.246227615602411 2216 13 -2.845976411093556 2216 14 -3.4314076508708276 2216 15 -3.00724376808601 2216 16 -2.8331672586969603 2216 17 -3.8479489284882185 2216 18 -3.079682904309898 2216 19 -3.297684762554134 2216 20 -2.9113877123616803 2216 21 -3.152474868199583 2216 22 -3.1351466163200747 2216 23 -3.0409027439552467 2216 24 -3.3601642714467204 2216 25 -3.3832236506662774 2216 26 -3.595963696406468 2216 27 -3.377465287866773 2216 28 -3.681350370089376 2216 29 -3.2429146208583113 2216 30 -3.6342590689412617 2216 31 -3.858244542860213 2216 32 -3.9046086444895565 2216 33 -3.7005228623224937 2216 34 -3.884517620898695 2216 35 -3.635674203684676 2216 36 -3.838613424672939 2216 37 -3.5209514015901853 2216 38 -3.302412187265759 2216 39 -3.282157399566053 2216 40 -3.2729041121052234 2216 41 -3.5365200831318653 2216 42 -3.391345018723264 2216 43 -3.2427326545103874 2216 44 -3.226228932391919 2216 45 -3.5289134306683536 2216 46 -3.5546374633587767 2216 47 -4.147926694375329 2216 48 -3.7843164779098792 2216 49 -3.378076913262838 2216 50 -3.295246342233746 2216 51 -3.3067574808594777 2216 52 -3.2942784357575547 2216 53 -3.121869060801089 2216 54 -3.314424856946715 2216 55 -3.293045715334202 2216 56 -3.3750293401378997 2216 57 -3.3626266038446033 2216 58 -3.0801166203946586 2216 59 -3.5799002983641834 2216 60 -3.31012511989368 2216 61 -3.5728828314963437 2216 62 -3.4692952792748386 2216 63 -3.4407975171271517 2216 64 -3.4028587243630284 2216 65 -3.4837167748142654 2216 66 -3.3075989807871835 2216 67 -3.7039627058268323 2216 68 -3.4412384492972556 2216 69 -3.7517067564238644 2216 70 -3.749654020267183 2216 71 -3.8175422004553354 2216 72 -3.83475557472439 2216 73 -3.637080480176781 2216 74 -3.572631659477487 2216 75 -3.5080605046236 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16087.0 3 5630.0 4 2873.0 5 1754.0 6 1306.0 7 1171.0 8 1255.0 9 1675.0 10 1912.0 11 1936.0 12 1935.0 13 1932.0 14 1834.0 15 1886.0 16 2072.0 17 2175.0 18 2246.0 19 2584.0 20 3317.0 21 4163.0 22 6533.0 23 8914.0 24 7142.0 25 4929.0 26 3578.0 27 3166.0 28 3400.0 29 3547.0 30 3987.0 31 4421.0 32 5229.0 33 6533.0 34 9416.0 35 20351.0 36 22124.0 37 15288.0 38 24671.0 39 34410.0 40 8445.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.87564785079776 20.51296092750721 16.350678458692055 16.260712763002978 2 13.341777991500608 25.691699604743086 37.36526665181032 23.601255751945985 3 18.751563574957782 34.89782037650885 29.993667521420974 16.35694852711239 4 9.099911928760154 20.717487033956356 35.5447695469224 34.637831490361094 5 8.311586689880475 43.35843815411099 30.224378411158277 18.105596744850253 6 22.928050378424892 43.36729704887255 15.576633485224805 18.128019087477753 7 19.264544885830468 43.33789490147013 19.717704097591493 17.679856115107913 8 26.176931708442318 31.695149176411714 17.719366151752936 24.408552963393035 9 26.53581582333181 19.86304851961785 20.114113198102544 33.4870224589478 10 17.746141845579984 33.93494606208237 24.578799407249853 23.740112685087798 11 24.7513565921779 30.378282014793502 20.652649850657575 24.217711542371028 12 28.287661585652135 34.91815080284203 24.432689599048484 12.361498012457355 13 29.603868211656277 27.57000571155847 24.69662235644819 18.12950372033706 14 24.030158928794894 27.192310098861217 32.879567638593414 15.89796333375047 15 24.805498432258723 29.44538708744165 30.680423172858134 15.068691307441492 16 14.556118159957773 33.156732028699345 32.918613516842285 19.368536294500597 17 16.383796442911823 30.689171172650536 30.76619319625759 22.160839188180052 18 14.630198681710493 24.719101123595504 41.55622277998014 19.09447741471386 19 15.332530610330105 26.94960046286885 44.890459584981784 12.827409341819262 20 16.48996473780092 22.68508745181745 43.52848732202754 17.296460488354093 21 16.982976360835465 23.424902839358467 45.14900571625183 14.443115083554241 22 15.961797085131668 22.431897791955965 43.31566272596485 18.290642396947522 23 14.965715134598396 24.92435437337274 45.511692820115876 14.598237671912994 24 17.71628299762292 23.773927186288002 44.32745840110096 14.182331414988115 25 14.44946596400144 24.173534333119616 45.129169468466074 16.247830234412874 26 17.88713890235701 22.381797707117947 46.166538936765875 13.56452445375917 27 14.145734930714129 22.62973505583534 46.89503894397698 16.329491069473555 28 14.564450665895068 23.931557091802024 47.44629437019543 14.057697872107482 29 15.165641388642292 23.172586946574736 45.990856579703795 15.670915085079173 30 14.35961804659456 25.186714528149906 46.86045875075273 13.59320867450281 31 16.825199485501383 22.24229131724939 44.68905282993788 16.243456367311353 32 13.033909039022367 24.865716452568783 46.165393546571174 15.934980961837672 33 15.479825326145175 23.41442818551083 45.47658048954029 15.62916599880371 34 14.20227530396028 22.568122287814223 46.53231166191016 16.69729074631534 35 15.975341362635989 24.15788095210173 45.80103434930985 14.065743335952435 36 16.48377853269899 22.59473110166808 44.86694839388123 16.0545419717517 37 15.587740901450294 23.218795199562177 46.88831554669481 14.305148352292719 38 16.549071421032675 23.60283166745498 45.72572228237713 14.122374629135216 39 15.290672900630565 23.43248515459162 45.57413321970423 15.702708725073592 40 15.090090970574321 23.991680903216224 46.09280015011904 14.825427976090417 41 14.282586854625276 24.338841521338853 45.04712647722253 16.33144514681334 42 14.377411448295813 26.217011778226894 43.881224810504634 15.524351962972663 43 14.713551337933191 23.402200895213152 43.3799182971404 18.50432946971326 44 15.118038208493124 25.34645259902974 43.11883568471543 16.41667350776171 45 15.649260791043492 22.917823105850342 42.08565598442618 19.34726011867998 46 14.413928434614693 25.27812306994707 43.08503568887742 17.22291280656081 47 17.122139045794814 24.573617653382325 39.60283798839005 18.70140531243281 48 14.810370034478176 25.489613547342994 39.40949283463767 20.290523583541166 49 17.06375110916532 24.904328383296264 39.14229760420291 18.88962290333551 50 14.942636567966384 25.084725886836708 40.04495260431936 19.92768494087755 51 15.330010749535816 27.290139743965604 37.567086875794 19.812762630704583 52 15.401784841744814 27.157661020791885 35.51975795575813 21.920796181705178 53 14.926493294296536 27.511169657620187 36.86436537191149 20.69797167617179 54 14.779909860960727 25.851845192258832 36.680647468797275 22.687597477983168 55 15.067213390298914 26.257197246576787 36.7134821578645 21.962107205259805 56 17.029311274950548 25.893059584216164 35.70479191262128 21.372837228212006 57 16.276097145291864 25.602076435660592 34.47031736786762 23.651509051179918 58 16.99312425975366 25.22915876744831 36.02199924167504 21.755717731122985 59 17.137585414288615 24.22482135318124 36.10463934451863 22.53295388801151 60 15.621738467612373 25.96130918642968 37.70263033425454 20.714322011703405 61 19.12979916036681 24.938434717346947 35.18563398559959 20.746132136686654 62 16.63219707484137 25.946806056743853 36.58129415398329 20.839702714431485 63 18.700207185020133 25.41573824322739 36.06309370235722 19.820960869395254 64 17.954190953123717 25.392785798335428 36.200014855297674 20.453008393243184 65 16.54827296362179 27.538753249799637 36.882538069081455 19.030435717497117 66 18.24301871449738 24.711971883295348 35.5458167019168 21.49919270029047 67 17.90591390492871 25.915703300011337 36.291523806917475 19.886858988142478 68 17.814752842197446 25.037140131671542 36.47395499397939 20.674152032151625 69 18.29507607149 26.343173893327915 35.651572248369895 19.710177786812192 70 19.509916221319582 24.872064520921214 36.37707052186729 19.240948735891912 71 16.781986630702477 26.273015128415622 35.35045541612916 21.594542824752747 72 18.31175329955589 25.34872083567899 35.89244386063677 20.447082004128355 73 16.581056252687542 23.934169891716508 37.75771080098511 21.72706305461084 74 15.898570669001971 26.61112501172865 36.57930128545961 20.911003033809777 75 17.322200592599543 26.510620665931782 35.81513708750615 20.352041653962523 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5540.0 1 3517.0 2 2612.0 3 3527.5 4 3325.0 5 2377.0 6 1473.0 7 2704.5 8 3892.0 9 5375.0 10 8331.0 11 8773.0 12 7742.0 13 5386.5 14 2091.0 15 1074.0 16 997.0 17 1054.0 18 1186.0 19 1291.5 20 1322.0 21 1402.0 22 1669.5 23 2001.0 24 2145.0 25 2206.0 26 2413.5 27 2840.5 28 3121.0 29 3361.5 30 4023.0 31 4856.5 32 5269.0 33 6383.0 34 7804.5 35 8431.0 36 8750.0 37 8878.5 38 8793.5 39 8596.5 40 8613.0 41 8661.5 42 8692.0 43 8388.5 44 8103.0 45 8341.5 46 10456.5 47 16010.0 48 19687.0 49 15859.0 50 11362.0 51 8441.0 52 6189.0 53 4941.5 54 3553.0 55 3206.5 56 3001.0 57 3087.0 58 3493.0 59 3684.5 60 3556.0 61 3283.5 62 2490.0 63 1474.0 64 979.0 65 771.0 66 474.0 67 382.0 68 379.0 69 339.5 70 279.0 71 213.5 72 169.0 73 195.5 74 214.5 75 149.0 76 91.0 77 72.0 78 33.5 79 21.0 80 28.0 81 26.0 82 15.0 83 6.0 84 6.0 85 4.0 86 1.0 87 0.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06801471306781535 2 0.017199122844734918 3 0.0011726674666864717 4 0.13759298275787935 5 0.09068628409042048 6 0.06332404320106948 7 0.026189573422664536 8 0.021889792711480806 9 0.04534314204521024 10 0.02736224088935101 11 0.015244677066924133 12 0.09185895155710695 13 0.08013227689024223 14 0.04495225288964808 15 0.017590012000297077 16 0.028144019200475323 17 0.021889792711480806 18 0.016026455378048445 19 0.012117563822426874 20 0.01133578551130256 21 0.025407795111540222 22 0.013681120444675504 23 0.01133578551130256 24 0.01993534693367002 25 0.015244677066924133 26 0.030880243289410424 27 0.029316686667161793 28 0.03166202160053474 29 0.0484702552897075 30 0.034789134845031995 31 0.017199122844734918 32 0.00977222888905393 33 0.014072009600237662 34 0.014462898755799819 35 0.005472448177870202 36 0.009381339733491773 37 0.006645115644556673 38 0.0023453349333729433 39 0.009381339733491773 40 0.011726674666864717 41 0.010163118044616087 42 0.006254226488994516 43 0.008599561422367459 44 0.007817783111243145 45 0.005081559022308044 46 0.0019544457778107862 47 0.004690669866745887 48 0.005081559022308044 49 0.001563556622248629 50 7.817783111243145E-4 51 7.817783111243145E-4 52 0.0023453349333729433 53 0.0 54 0.0 55 0.0 56 0.008208672266805302 57 0.001563556622248629 58 0.0 59 0.005863337333432359 60 0.001563556622248629 61 3.9088915556215724E-4 62 0.017980901155859232 63 0.006645115644556673 64 0.010163118044616087 65 0.016417344533610604 66 0.014072009600237662 67 0.01563556622248629 68 0.015244677066924133 69 0.005863337333432359 70 0.013290231289113346 71 0.006645115644556673 72 0.013681120444675504 73 0.006645115644556673 74 0.01680823368917276 75 0.0019544457778107862 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 255827.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.34678754883967 #Duplication Level Percentage of deduplicated Percentage of total 1 86.19912736358857 39.950526428156124 2 6.929009463019897 6.422746590129656 3 2.4364360469812807 3.387629515373286 4 1.213647832400676 2.249947129895357 5 0.6708777388355524 1.5546514016528645 6 0.47492080882257415 1.3206632297413767 7 0.3266999032261508 1.0599043704934226 8 0.2194180288909883 0.8135456615516641 9 0.2144428405128779 0.8944863093558056 >10 1.1208373097658957 9.345891233071436 >50 0.08835641373489103 2.889070858631443 >100 0.07842872679838642 7.7129311678991685 >500 0.014891530404756917 4.857908922667746 >1k 0.010920455630155071 11.128375185039033 >5k 0.001985537387300922 6.41172199634164 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8135 3.179883280498149 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5800 2.267157102260512 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4616 1.8043443420749177 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3785 1.479515453802765 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2704 1.056964276640073 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2233 0.8728554843702971 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2166 0.8466659109476325 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1627 0.6359766560996298 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1532 0.5988421863212249 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG 1446 0.5652257189428794 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 1404 0.5488083744092688 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 1344 0.5253550250755393 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1329 0.519491687742107 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 989 0.3865893748509735 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 951 0.3717355869396115 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 811 0.3170111051609095 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 809 0.3162293268497852 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 793 0.3099751003607907 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 769 0.3005937606272989 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 672 0.26267751253776966 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 657 0.2568141752043373 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 654 0.2556415077376508 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA 647 0.25290528364871573 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 635 0.24821461378196985 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 596 0.23296993671504573 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTG 559 0.2185070379592459 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 511 0.19974435849226235 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA 505 0.19739902355888939 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 488 0.19075390791433272 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA 443 0.17316389591403566 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 433 0.16925500435841406 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 424 0.16573700195835467 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 401 0.15674655138042506 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 397 0.15518299475817643 No Hit GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA 384 0.15010143573586837 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 377 0.14736521164693328 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 377 0.14736521164693328 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT 362 0.14150187431350092 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 362 0.14150187431350092 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 351 0.1372020936023172 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 351 0.1372020936023172 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 335 0.13094786711332268 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 329 0.12860253217994974 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 328 0.12821164302438756 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 300 0.11726674666864717 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 296 0.11570319004639855 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 292 0.11413963342414991 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC 278 0.10866718524627972 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 276 0.1078854069351554 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 271 0.10593096115734463 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 267 0.10436740453509598 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 259 0.10124029129059872 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.9088915556215724E-4 0.0 10 0.0 0.0 0.0 3.9088915556215724E-4 0.0 11 0.0 0.0 0.0 3.9088915556215724E-4 0.0 12 0.0 0.0 0.0 3.9088915556215724E-4 0.0 13 0.0 0.0 0.0 3.9088915556215724E-4 0.0 14 0.0 0.0 0.0 3.9088915556215724E-4 0.0 15 0.0 0.0 0.0 3.9088915556215724E-4 0.0 16 0.0 0.0 0.0 3.9088915556215724E-4 0.0 17 0.0 0.0 0.0 3.9088915556215724E-4 0.0 18 0.0 0.0 0.0 3.9088915556215724E-4 0.0 19 0.0 0.0 0.0 3.9088915556215724E-4 0.0 20 0.0 0.0 0.0 3.9088915556215724E-4 0.0 21 0.0 0.0 0.0 0.0011726674666864717 0.0 22 0.0 0.0 0.0 0.001563556622248629 0.0 23 0.0 0.0 0.0 0.003127113244497258 0.0 24 0.0 0.0 0.0 0.010554007200178246 0.0 25 0.0 0.0 0.0 0.011726674666864717 0.0 26 0.0 0.0 0.0 0.021889792711480806 0.0 27 0.0 0.0 0.0 0.025407795111540222 0.0 28 0.0 0.0 0.0 0.029316686667161793 0.0 29 0.0 0.0 0.0 0.03713446977840494 0.0 30 0.0 0.0 0.0 0.060196929956572214 0.0 31 0.0 0.0 0.0 0.08013227689024223 0.0 32 0.0 0.0 0.0 0.09459517564604206 0.0 33 0.0 0.0 0.0 0.10827629609071755 0.0 34 0.0 0.0 0.0 0.13016608880219835 0.0 35 0.0 0.0 0.0 0.15127410320255485 0.0 36 0.0 0.0 0.0 0.18137256818084097 0.0 37 0.0 0.0 0.0 0.2110801440035649 0.0 38 0.0 0.0 0.0 0.2513417270264671 0.0 39 0.0 0.0 0.0 0.29668486907167735 0.0 40 0.0 0.0 0.0 0.3545364640948766 0.0 41 0.0 0.0 0.0 0.4147333940514488 0.0 42 0.0 0.0 0.0 0.4655489842745293 0.0 43 0.0 0.0 0.0 0.5175372419642962 0.0 44 0.0 0.0 0.0 0.5722617237429982 0.0 45 0.0 0.0 0.0 0.6277679838328245 0.0 46 0.0 0.0 0.0 0.6907011378783319 0.0 47 0.0 0.0 0.0 0.7579340726350229 0.0 48 0.0 0.0 0.0 0.8263396748584004 0.0 49 0.0 0.0 0.0 0.9002177252596482 0.0 50 0.0 0.0 0.0 0.9639326576162798 0.0 51 0.0 0.0 0.0 1.0331200381507817 0.0 52 0.0 0.0 0.0 1.0980076379740997 0.0 53 0.0 0.0 0.0 1.178530804019904 0.0 54 0.0 0.0 0.0 1.242636625532098 0.0 55 0.0 0.0 0.0 1.3036153337997944 0.0 56 0.0 0.0 0.0 1.3790569408232907 0.0 57 0.0 0.0 0.0 1.4548894370023493 0.0 58 0.0 0.0 0.0 1.5514390584262021 0.0 59 0.0 0.0 0.0 1.6495522364723036 0.0 60 0.0 0.0 0.0 1.7507925277629022 0.0 61 0.0 0.0 0.0 1.8504692624312524 0.0 62 0.0 0.0 0.0 1.9564002235885969 0.0 63 0.0 0.0 0.0 2.036532500478839 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAGGG 30 8.977737E-6 57.637554 2 TACAAGG 45 2.9596777E-8 53.79505 2 TAGACCC 20 0.007277727 51.88397 4 AATCTCG 20 0.0073570604 51.741932 26 GCGTAGA 20 0.0073570604 51.741932 15 CTTAACG 20 0.0073627518 51.731815 21 AGGGGTC 30 6.282767E-4 46.02883 5 GGGCGTT 30 6.319217E-4 45.97485 33 TAGGGCG 30 6.319217E-4 45.97485 31 TAGATGT 35 0.001328851 39.530643 4 CGTTTAT 35 0.0013481823 39.414722 36 CTAGGAA 55 7.340246E-6 37.659954 12 ACTTAAT 50 1.8188446E-4 34.62326 1 ATAGGGG 50 1.829364E-4 34.589314 3 ATACCTT 40 0.0025821396 34.52838 6 AGGTGTT 40 0.00259456 34.49462 28 TAGCATC 40 0.0025970498 34.487877 21 AGCATCA 40 0.0025970498 34.487877 22 CTGCTCA 45 0.004601908 30.685888 9 TGTATAG 45 0.004601908 30.685888 10 >>END_MODULE