FastQCFastQC Report
Mon 6 Feb 2017
SRR2077271_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077271_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3980345
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47300.035.7649961
GTATCAA98400.035.0504531
TCAACGC124500.027.01764
TATCAAC126000.026.8859332
ATCAACG125750.026.7757953
CAACGCA127550.026.3171235
AACGCAG132450.025.324396
ACGCAGA152950.021.6835827
CGCAGAG155650.021.1985658
GTACATG157650.021.1546761
TACATGG157450.021.0116462
GAGTACT77950.020.613512
AGAGTAC152950.020.13185911
AGTACTT83900.019.810213
GTACTTT83050.019.30758914
CAGAGTA162550.019.30302610
ACATGGG163350.019.091933
GCAGAGT167950.019.051699
GTGGTAT26150.018.6153261
TACTTTT93450.017.15885715
Produced by FastQC (version 0.11.5)