FastQCFastQC Report
Mon 6 Feb 2017
SRR2077271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3980345
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58800.040.7849731
GTATCAA120800.034.4049031
TATCAAC163800.025.271062
ATCAACG163150.025.203763
TCAACGC164700.025.0295624
CAACGCA170550.024.1774655
AACGCAG176150.023.480176
GTGGTAT24350.022.02811
GTACATG153750.021.1121271
TACATGG154450.020.598862
ACGCAGA204750.020.0662637
TGGTATC25950.019.989282
CGCAGAG208000.019.7197768
GAGTACT134800.019.54363612
ACATGGG160400.019.2538553
AGAGTAC205650.019.19058811
AGTACTT142050.018.756913
GTACTTT144450.018.55749114
CAGAGTA215200.018.53149410
GCAGAGT221150.018.2817969
Produced by FastQC (version 0.11.5)