FastQCFastQC Report
Mon 6 Feb 2017
SRR2077270_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077270_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4163828
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33950.035.5983771
GTATCAA67350.034.504791
TATCAAC89250.025.6314182
TCAACGC89100.025.5199764
ATCAACG89850.025.2685533
CAACGCA91300.024.9044385
AACGCAG95850.023.6323156
GTACATG116950.020.5794831
ACGCAGA112450.019.9618847
TACATGG118100.019.7498662
TGCGATA2303.4742698E-1019.49372113
CGCAGAG114700.019.4209298
AGAGTAC113500.018.68556611
GTGGTAT20200.018.290871
ACATGGG124400.018.001013
GAGTACT62850.017.94237512
CAGAGTA122250.017.57297510
GCAGAGT128600.016.8927569
TGGTATC20350.016.7856482
AGTACTT68500.016.61508813
Produced by FastQC (version 0.11.5)