##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077270_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4163828
Sequences flagged as poor quality	0
Sequence length	75
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.761979361299264	31.0	30.0	34.0	25.0	34.0
2	29.8858658426813	31.0	30.0	34.0	25.0	34.0
3	29.932249603009538	31.0	30.0	34.0	25.0	34.0
4	33.37331008869723	37.0	35.0	37.0	27.0	37.0
5	33.38724246054352	37.0	35.0	37.0	27.0	37.0
6	33.426308435410874	37.0	35.0	37.0	27.0	37.0
7	33.29937427770792	37.0	35.0	37.0	27.0	37.0
8	33.21424468061601	37.0	35.0	37.0	26.0	37.0
9	34.64887670672275	39.0	35.0	39.0	27.0	39.0
10	34.554445572679754	39.0	35.0	39.0	26.0	39.0
11	34.595095186448624	39.0	35.0	39.0	27.0	39.0
12	34.503625029660206	39.0	35.0	39.0	26.0	39.0
13	34.48630106719106	39.0	35.0	39.0	26.0	39.0
14	35.58901112149685	40.0	36.0	41.0	25.0	41.0
15	35.601046200755654	40.0	36.0	41.0	25.0	41.0
16	35.57615420233497	40.0	36.0	41.0	25.0	41.0
17	35.49190192294206	40.0	36.0	41.0	25.0	41.0
18	35.448687361725796	40.0	36.0	41.0	25.0	41.0
19	35.531775568059004	40.0	36.0	41.0	25.0	41.0
20	35.504465121998315	40.0	36.0	41.0	25.0	41.0
21	35.4766347217032	40.0	36.0	41.0	25.0	41.0
22	35.40288575800922	40.0	36.0	41.0	25.0	41.0
23	35.350080022517744	40.0	36.0	41.0	24.0	41.0
24	35.314504105356896	40.0	36.0	41.0	24.0	41.0
25	35.33227020904802	40.0	36.0	41.0	24.0	41.0
26	35.27120188441982	40.0	35.0	41.0	24.0	41.0
27	35.249338589394185	40.0	35.0	41.0	24.0	41.0
28	35.20772327771464	40.0	35.0	41.0	24.0	41.0
29	35.16733952507164	40.0	35.0	41.0	24.0	41.0
30	35.083542355736114	40.0	35.0	41.0	24.0	41.0
31	35.06280278628224	40.0	35.0	41.0	24.0	41.0
32	34.99724412247576	40.0	35.0	41.0	23.0	41.0
33	34.87965112872097	40.0	35.0	41.0	22.0	41.0
34	34.84825958228822	40.0	35.0	41.0	22.0	41.0
35	34.7995476758406	40.0	35.0	41.0	22.0	41.0
36	34.72296934455506	40.0	35.0	41.0	21.0	41.0
37	34.646991902643435	39.0	35.0	41.0	21.0	41.0
38	34.56833039212955	39.0	34.0	41.0	19.0	41.0
39	34.51729154038063	39.0	34.0	41.0	19.0	41.0
40	34.473394193996484	39.0	34.0	41.0	19.0	41.0
41	34.38517465178677	39.0	34.0	41.0	18.0	41.0
42	34.2980980482383	39.0	34.0	41.0	16.0	41.0
43	34.20677655272984	39.0	34.0	41.0	16.0	41.0
44	34.10462824112812	39.0	34.0	41.0	15.0	41.0
45	34.0070041317749	39.0	33.0	41.0	15.0	41.0
46	33.91001213306602	39.0	33.0	41.0	13.0	41.0
47	33.79994538679311	39.0	33.0	41.0	9.0	41.0
48	33.692106878574236	39.0	33.0	41.0	9.0	41.0
49	33.58613324085433	39.0	33.0	41.0	8.0	41.0
50	33.45698141229657	38.0	33.0	40.0	8.0	41.0
51	33.34854345568549	38.0	33.0	40.0	7.0	41.0
52	33.24091485046933	38.0	33.0	40.0	6.0	41.0
53	33.14657353761971	38.0	33.0	40.0	2.0	41.0
54	33.14010040760569	38.0	33.0	40.0	2.0	41.0
55	33.03688000561022	38.0	33.0	40.0	2.0	41.0
56	32.89352874326221	38.0	32.0	40.0	2.0	41.0
57	32.78910680268253	38.0	32.0	40.0	2.0	41.0
58	32.68078508526289	38.0	32.0	40.0	2.0	41.0
59	32.55432693185213	37.0	32.0	40.0	2.0	41.0
60	32.51627276631023	37.0	32.0	40.0	2.0	41.0
61	32.39896868938871	37.0	32.0	40.0	2.0	41.0
62	32.2390427750618	37.0	32.0	40.0	2.0	41.0
63	32.03913826411658	37.0	32.0	40.0	2.0	41.0
64	31.886696328474663	36.0	31.0	40.0	2.0	41.0
65	31.723803913129938	36.0	31.0	39.0	2.0	41.0
66	31.548722713810466	36.0	31.0	39.0	2.0	41.0
67	31.37208885669629	36.0	31.0	39.0	2.0	41.0
68	31.175881424496882	35.0	31.0	39.0	2.0	41.0
69	31.207547477945774	35.0	31.0	39.0	2.0	41.0
70	31.103636365383007	35.0	31.0	39.0	2.0	41.0
71	30.935067442747396	35.0	31.0	39.0	2.0	40.0
72	30.730836384211837	35.0	31.0	38.0	2.0	40.0
73	30.52152658563226	35.0	31.0	37.0	2.0	40.0
74	30.30547803607642	35.0	31.0	37.0	2.0	39.0
75	30.086922898832515	35.0	31.0	37.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6115138124809043
1101	2	0.7264121780411372
1101	3	0.6130559506091835
1101	4	0.6888821227294457
1101	5	0.6752146812287165
1101	6	0.657624633240637
1101	7	0.6743884579392443
1101	8	0.6990452574747863
1101	9	0.7714785684001555
1101	10	0.8753405701350232
1101	11	0.7674521522873974
1101	12	0.8091320047213131
1101	13	0.7577217695755891
1101	14	0.8965796838457081
1101	15	0.9342557489982397
1101	16	0.916120643774839
1101	17	0.899711459512119
1101	18	0.9582238595058854
1101	19	0.9278941403917713
1101	20	0.8881697636822636
1101	21	0.9469428381179625
1101	22	1.006141855969581
1101	23	0.9543474758446351
1101	24	0.9655636903611722
1101	25	0.8684188462597859
1101	26	0.8678197412753477
1101	27	0.9417868694979035
1101	28	0.9448122883508532
1101	29	0.902982027105466
1101	30	0.9541365394961332
1101	31	1.0545172969379948
1101	32	0.9785406593206218
1101	33	0.9837348379682709
1101	34	0.8975399984064012
1101	35	0.8315103364995196
1101	36	0.9937830100755605
1101	37	1.0075078932547825
1101	38	0.923617528384284
1101	39	0.9291690697193431
1101	40	0.9514125023193998
1101	41	0.9897350708949801
1101	42	0.8180496939258219
1101	43	0.826959425187269
1101	44	0.8679000616654236
1101	45	0.9161133942826609
1101	46	0.9347634354213241
1101	47	0.8716845822290509
1101	48	0.8865346580284523
1101	49	0.8811651602698092
1101	50	0.849545620593922
1101	51	0.8405444754167419
1101	52	0.8337248555336743
1101	53	0.8384230142341735
1101	54	0.7983410155308093
1101	55	0.686261891323916
1101	56	0.6551677463860912
1101	57	0.6930092383797941
1101	58	0.6398769837364284
1101	59	0.683741175225606
1101	60	0.7562986862027898
1101	61	0.6547821410469297
1101	62	0.6655252778466689
1101	63	0.749166483487059
1101	64	0.6680225917845988
1101	65	0.7197033669982495
1101	66	0.6308174474716886
1101	67	0.6526015615483516
1101	68	0.6207523378835091
1101	69	0.6942205642098429
1101	70	0.7352666577610663
1101	71	0.6971377417262836
1101	72	0.7459635140016871
1101	73	0.7170561697502684
1101	74	0.7504194627294432
1101	75	0.767966005606521
1102	1	0.7842375916923707
1102	2	0.845816696401112
1102	3	0.8008906339588009
1102	4	0.9042672171509523
1102	5	0.7614929942277939
1102	6	0.7325596413639275
1102	7	0.700814221400158
1102	8	0.8189747114679591
1102	9	1.0092636946268456
1102	10	0.9572417363069263
1102	11	0.9228664371782287
1102	12	0.8702134590930015
1102	13	0.8915105751400887
1102	14	1.052885305869843
1102	15	0.998326393568
1102	16	0.9919704909078177
1102	17	1.1126208072617985
1102	18	1.108946854096338
1102	19	1.1421673216991977
1102	20	1.0556236612445744
1102	21	1.0663461140879917
1102	22	1.1486951531391867
1102	23	1.1926200589413085
1102	24	1.1191378404506196
1102	25	1.1345607463963248
1102	26	1.1852619150767438
1102	27	1.1687565771581205
1102	28	1.102657438576891
1102	29	1.1412062864100534
1102	30	1.2389794883366108
1102	31	1.1752960797545953
1102	32	1.2227747211336109
1102	33	1.303829177813114
1102	34	1.2452144372661067
1102	35	1.2720737675850415
1102	36	1.1993325174640646
1102	37	1.2019451026471586
1102	38	1.2283995933880405
1102	39	1.2741613698782643
1102	40	1.275546421291196
1102	41	1.2703995776494779
1102	42	1.270781687405453
1102	43	1.2268788976125933
1102	44	1.1714294093617852
1102	45	1.1705141659310527
1102	46	1.1701429582416054
1102	47	1.2173534413602312
1102	48	1.2602914171797437
1102	49	1.1973132814151484
1102	50	1.1932126012832711
1102	51	1.23850669190562
1102	52	1.2359343632177655
1102	53	1.2364089655475539
1102	54	1.1946599124914599
1102	55	1.2025062821598098
1102	56	1.1916407302415166
1102	57	1.1631714191861988
1102	58	1.2081522252887424
1102	59	1.1717916487664155
1102	60	1.2273521006100054
1102	61	1.1738979797508549
1102	62	1.1833049779352933
1102	63	1.1615802833022713
1102	64	1.1086049693167226
1102	65	1.2434741560967915
1102	66	1.2153420623612128
1102	67	1.1657372080374735
1102	68	1.1602452093218005
1102	69	1.1132055456049272
1102	70	1.1398677401984827
1102	71	1.0941081427953812
1102	72	1.071131800248743
1102	73	1.0240459099129566
1102	74	1.0732930029892032
1102	75	1.1102253067264591
1103	1	0.7020873162490346
1103	2	0.7726510370126256
1103	3	0.7590930641378648
1103	4	0.8118399887977219
1103	5	0.8036858890194196
1103	6	0.7762518323539638
1103	7	0.7979025116983962
1103	8	0.8512598784340071
1103	9	0.9413911363490968
1103	10	0.897330943461796
1103	11	0.7821386953220042
1103	12	0.815965605692142
1103	13	0.8848671237990473
1103	14	0.9717203011371538
1103	15	1.0256203252144047
1103	16	1.010486201653471
1103	17	0.9901080142087082
1103	18	1.087062810740207
1103	19	1.0975709806039973
1103	20	1.0212354910278876
1103	21	1.000181773749496
1103	22	1.0796786152552613
1103	23	1.0166602178356499
1103	24	1.101836878269971
1103	25	1.0730011962553405
1103	26	1.0420919695668758
1103	27	1.0805595173250353
1103	28	1.0702646586394806
1103	29	1.0413683093043176
1103	30	1.0618609987036791
1103	31	1.003555172443555
1103	32	1.15849536168664
1103	33	1.1066243971479537
1103	34	1.0752899005249859
1103	35	1.163390185755361
1103	36	1.1115259747278685
1103	37	1.1217789827575757
1103	38	1.1196618493881374
1103	39	1.1591957612619055
1103	40	1.178212242589737
1103	41	1.1552358159484086
1103	42	1.127873672898133
1103	43	1.1618879483123834
1103	44	1.147250023272818
1103	45	1.0518769116449889
1103	46	1.0495940589883688
1103	47	1.1044686854330479
1103	48	1.1370899452639023
1103	49	1.0859942733707655
1103	50	1.1101917817914853
1103	51	1.1452536376363298
1103	52	1.155640712022489
1103	53	1.2076310198730411
1103	54	1.1329918663002587
1103	55	1.1947496608528922
1103	56	1.212368749776985
1103	57	1.116791970158232
1103	58	1.149097430220806
1103	59	1.1107323382709566
1103	60	1.0898170322605623
1103	61	1.1341144920451214
1103	62	1.1112545735599895
1103	63	1.1487703903856428
1103	64	1.1519423097839265
1103	65	1.2208242871272965
1103	66	1.2614672337367772
1103	67	1.1745287241810978
1103	68	1.1613370136851984
1103	69	1.1959314167567001
1103	70	1.2274889835534069
1103	71	1.1634482554924368
1103	72	1.2137385131542366
1103	73	1.2497019706634838
1103	74	1.2456192689361067
1103	75	1.311444195950127
1104	1	0.5376079578848056
1104	2	0.5742813541169589
1104	3	0.6032894615714142
1104	4	0.5906427909769576
1104	5	0.5466072429749076
1104	6	0.5183690146383668
1104	7	0.5303428793055005
1104	8	0.5224974022041877
1104	9	0.5643576890926383
1104	10	0.6450585249548979
1104	11	0.6479757514682944
1104	12	0.6197505443093192
1104	13	0.6052928447223991
1104	14	0.7158975894934656
1104	15	0.6715611481052122
1104	16	0.7261202100815822
1104	17	0.7508384149733374
1104	18	0.7863632527639624
1104	19	0.8184810153174666
1104	20	0.7953115391193393
1104	21	0.7620426472120556
1104	22	0.7554749641328797
1104	23	0.8168306234750347
1104	24	0.8777153305506431
1104	25	0.786693901598639
1104	26	0.8369166958792889
1104	27	0.8177990226140679
1104	28	0.7473255407631569
1104	29	0.7153640504088443
1104	30	0.7955782349476337
1104	31	0.76495719332069
1104	32	0.8670871793416879
1104	33	0.835748867995612
1104	34	0.7961504260097527
1104	35	0.7971057740869156
1104	36	0.8033362716510268
1104	37	0.8572296168589375
1104	38	0.8755190761738447
1104	39	0.89341012573108
1104	40	0.8607532238717539
1104	41	0.8438017134743205
1104	42	0.9134851078006108
1104	43	0.8679572623822551
1104	44	0.8576459457243573
1104	45	0.8652627843219136
1104	46	0.9138250233007241
1104	47	0.9812904430120994
1104	48	0.9220689458778395
1104	49	0.8464940382558197
1104	50	0.7894074291412991
1104	51	0.8316782789854429
1104	52	0.8793060419000582
1104	53	0.9059102592043402
1104	54	0.8633276246197212
1104	55	0.8439975181835351
1104	56	0.9844262249375788
1104	57	0.8239510381602955
1104	58	0.9031194382825447
1104	59	0.9090484352709112
1104	60	0.9986683386335358
1104	61	0.9840002830928896
1104	62	0.967697211645465
1104	63	0.9415284477191932
1104	64	0.9351519842177538
1104	65	0.9247444647415897
1104	66	0.8927631692949696
1104	67	0.8940681412876579
1104	68	0.9290747547498697
1104	69	1.0139941510572683
1104	70	0.997979480847377
1104	71	0.9448134895994684
1104	72	0.9993304749058076
1104	73	1.0077604299726914
1104	74	1.0359105834413391
1104	75	0.9574770432514974
1105	1	0.5981305383619393
1105	2	0.6280921119339951
1105	3	0.6606116528711041
1105	4	0.6166013171627185
1105	5	0.6473837375502356
1105	6	0.7093117095619448
1105	7	0.6682689839592584
1105	8	0.7280895955874271
1105	9	0.8102921614335088
1105	10	0.7956014572543921
1105	11	0.7873056481837253
1105	12	0.7913950980574782
1105	13	0.7835599261148047
1105	14	0.8827472799374121
1105	15	0.8841410485412737
1105	16	0.9000684822230269
1105	17	0.8688789846755398
1105	18	0.8881104585740971
1105	19	0.8894753897314658
1105	20	0.9271591646160431
1105	21	0.9358954853053092
1105	22	1.014756343889701
1105	23	0.9937608840676546
1105	24	1.0005945739242108
1105	25	0.9758242797630317
1105	26	0.9800927893711702
1105	27	1.0418227304407779
1105	28	1.0075718647850067
1105	29	1.115719928253263
1105	30	1.0433200012030426
1105	31	0.9725271782893614
1105	32	1.057386299041653
1105	33	1.0341907629846716
1105	34	0.990662782207572
1105	35	1.0611111392291193
1105	36	1.114722130297018
1105	37	1.0134271560371246
1105	38	1.063508710510753
1105	39	1.0633358434397664
1105	40	1.05116145091921
1105	41	1.1675751162097256
1105	42	1.1309562357454865
1105	43	1.1651233548323603
1105	44	1.2252093451856112
1105	45	1.2040697471007746
1105	46	1.19957995685035
1105	47	1.174786975388983
1105	48	1.2528878183794419
1105	49	1.165647168046128
1105	50	1.2183218993986173
1105	51	1.1851520411332856
1105	52	1.2696397634186738
1105	53	1.1037522033056604
1105	54	1.1905412108563809
1105	55	1.130259573010676
1105	56	1.186478987130073
1105	57	1.182524103807019
1105	58	1.134491939882416
1105	59	1.1084867138118284
1105	60	1.0776455944488745
1105	61	1.2195070509109698
1105	62	1.1758351501212019
1105	63	1.2605747025374043
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2211	75	-0.5814168927376855
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2212	3	-0.18358608392482267
2212	4	-0.13973829824033857
2212	5	-0.2154892130870607
2212	6	-0.13410581240542285
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2212	13	-0.3154197397832874
2212	14	-0.25994940898284113
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2212	51	-0.17406114270225714
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2212	56	-0.16007095080959743
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2212	59	-0.14375914447632
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2212	72	-0.37071706647794045
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2212	74	-0.28080668801731434
2212	75	-0.27970549026724356
2213	1	-0.3711761846002659
2213	2	-0.35276677010225654
2213	3	-0.31502076410560775
2213	4	-0.30676416284745045
2213	5	-0.45030754317175337
2213	6	-0.3586637177765084
2213	7	-0.28132487135111006
2213	8	-0.4419501110577073
2213	9	-0.4515117226178873
2213	10	-0.5019942666039796
2213	11	-0.5083142699641883
2213	12	-0.48054823669998115
2213	13	-0.4864814168547582
2213	14	-0.7478983419532952
2213	15	-0.5421565679392515
2213	16	-0.5127246608154863
2213	17	-0.6351344932580645
2213	18	-0.6638343690492121
2213	19	-0.6206733409514982
2213	20	-0.5897544450753074
2213	21	-0.6904185840811223
2213	22	-0.5624486481102409
2213	23	-0.5155286417972533
2213	24	-0.6635000746672759
2213	25	-0.7538124024165853
2213	26	-0.6361413646199594
2213	27	-0.711467335187038
2213	28	-0.7478124719589019
2213	29	-0.6550274999154553
2213	30	-0.8004548826963713
2213	31	-0.6821160155648727
2213	32	-0.578278288521652
2213	33	-0.6122745072621498
2213	34	-0.6716546387299331
2213	35	-0.7441814961005164
2213	36	-0.8575639583899743
2213	37	-0.7390913489989472
2213	38	-0.7186314808002265
2213	39	-0.6147193648015659
2213	40	-0.6487472063129616
2213	41	-0.6359917348161659
2213	42	-0.6294473356558683
2213	43	-0.5529314902794411
2213	44	-0.6155510704676956
2213	45	-0.6265073060717015
2213	46	-0.64452510593744
2213	47	-0.6525475776411511
2213	48	-0.658452851365908
2213	49	-0.5530473005944998
2213	50	-0.620027358584295
2213	51	-0.6245321975656566
2213	52	-0.5794592046210028
2213	53	-0.7123656147431277
2213	54	-0.5916211717037143
2213	55	-0.6248761096661184
2213	56	-0.6133912501011025
2213	57	-0.656040073061277
2213	58	-0.5950188798085279
2213	59	-0.5808450337064492
2213	60	-0.5653672554051354
2213	61	-0.6765189895111767
2213	62	-0.6688189555512842
2213	63	-0.6597764885498698
2213	64	-0.6372992581644645
2213	65	-0.6993921733630515
2213	66	-0.7079035713230226
2213	67	-0.6539562299372612
2213	68	-0.5765202734917381
2213	69	-0.6820075479347736
2213	70	-0.6819863474281469
2213	71	-0.6424908896589763
2213	72	-0.6304432459251466
2213	73	-0.7575745174365878
2213	74	-0.8250842452047991
2213	75	-0.8538915517659618
2214	1	-0.6266086449156347
2214	2	-0.6445144730554624
2214	3	-0.5844936753155103
2214	4	-0.6388482763870229
2214	5	-0.594569480895025
2214	6	-0.5309351716604311
2214	7	-0.6083390552836718
2214	8	-0.48833632448648245
2214	9	-0.6672046905328699
2214	10	-0.6406416686805372
2214	11	-0.7295197971320917
2214	12	-0.7195360186277355
2214	13	-0.8611225377710099
2214	14	-0.9919671617462811
2214	15	-0.7848664344212182
2214	16	-0.8285198268588658
2214	17	-0.7203746168809246
2214	18	-1.099495821802293
2214	19	-0.8285403256731456
2214	20	-0.824069719014318
2214	21	-0.7736520525225927
2214	22	-0.7753256079831914
2214	23	-0.8090994983948718
2214	24	-0.8187534401519798
2214	25	-0.8826923948440282
2214	26	-0.820790259810039
2214	27	-0.8073119273823224
2214	28	-0.8376920155997922
2214	29	-0.8124220898105108
2214	30	-0.9312471962208448
2214	31	-0.9741107480977149
2214	32	-1.0525803556888533
2214	33	-0.915584787587008
2214	34	-0.9107369915460026
2214	35	-0.9294318302793343
2214	36	-0.8904254799367592
2214	37	-0.7864095582090798
2214	38	-0.9298935238151529
2214	39	-0.8103138769517315
2214	40	-0.7256171497277961
2214	41	-0.7912513433142152
2214	42	-0.8106300883111004
2214	43	-0.6972595608888668
2214	44	-0.747913372681765
2214	45	-0.7756888459462488
2214	46	-0.9547841184429942
2214	47	-0.7676601326057693
2214	48	-0.8259429320249936
2214	49	-0.8053464140413951
2214	50	-0.8703510964994052
2214	51	-0.8166658414508703
2214	52	-0.782638676594253
2214	53	-0.7572141739858935
2214	54	-0.7250227418904558
2214	55	-0.8037016942322879
2214	56	-0.7480385167258063
2214	57	-0.7506756088486277
2214	58	-0.741492760213962
2214	59	-0.790557151784995
2214	60	-0.7333841818073488
2214	61	-0.936802077444213
2214	62	-0.8726098768622563
2214	63	-0.7964534879776899
2214	64	-0.822099406498463
2214	65	-0.880471270469183
2214	66	-0.7364835266820329
2214	67	-0.7411478118925174
2214	68	-0.7541652611797609
2214	69	-0.7273132249199286
2214	70	-0.6744919758814092
2214	71	-0.6679332356783334
2214	72	-0.6929916736792876
2214	73	-0.7342072414137846
2214	74	-0.7818265779825104
2214	75	-0.8248665556662296
2215	1	-0.8985585725752294
2215	2	-0.9492612113056751
2215	3	-1.0018314544021294
2215	4	-1.1937635285195967
2215	5	-0.9784346580389496
2215	6	-0.8718959988840567
2215	7	-0.8380472499264471
2215	8	-0.9398923526012055
2215	9	-0.896919294366846
2215	10	-0.9539267403589946
2215	11	-0.9658845154513642
2215	12	-0.9684313956358324
2215	13	-1.072460588402599
2215	14	-1.2708167617738084
2215	15	-1.288634093050426
2215	16	-1.3203910251708848
2215	17	-1.6752896064146938
2215	18	-1.5050207048529103
2215	19	-1.3159317886349058
2215	20	-1.1736005250477746
2215	21	-1.4002276481428666
2215	22	-1.2549385382326932
2215	23	-1.3322875849902545
2215	24	-1.2563251248971312
2215	25	-1.2291110267011334
2215	26	-1.2408386594405556
2215	27	-1.3263826872344495
2215	28	-1.4117750222368457
2215	29	-1.265063897227357
2215	30	-1.3489940063296615
2215	31	-1.215585283123687
2215	32	-1.3416810564782793
2215	33	-1.1689510873074553
2215	34	-1.3315291466603583
2215	35	-1.1958435491115864
2215	36	-1.355928197422699
2215	37	-1.542947239235076
2215	38	-1.2983371187153665
2215	39	-1.495259111859383
2215	40	-1.356269280259788
2215	41	-1.4297365069378571
2215	42	-1.3244284427379043
2215	43	-1.2657591828022277
2215	44	-1.2666208337614506
2215	45	-1.2560937118843256
2215	46	-1.2950816388849162
2215	47	-1.3124492186967203
2215	48	-1.3816555498553598
2215	49	-1.3447555404670766
2215	50	-1.4683867639600372
2215	51	-1.3891811999760186
2215	52	-1.1376701264732532
2215	53	-1.1987151602780806
2215	54	-1.236387673293084
2215	55	-1.3456166363869997
2215	56	-1.1392116851275027
2215	57	-1.1432263750396174
2215	58	-1.1101188167763674
2215	59	-1.1856604896450413
2215	60	-1.1655599506716179
2215	61	-1.1990944666609558
2215	62	-1.3445512500319197
2215	63	-1.2047078853337894
2215	64	-1.2429076068449731
2215	65	-1.2733894524477982
2215	66	-1.385789830764729
2215	67	-1.3600874125424873
2215	68	-1.3406368338464034
2215	69	-1.3399103745167054
2215	70	-1.3449185901191107
2215	71	-1.3778416027150016
2215	72	-1.39689596380272
2215	73	-1.221618221252939
2215	74	-1.2411154288138597
2215	75	-1.3497759551835422
2216	1	-1.1879347547467525
2216	2	-1.0957082013820454
2216	3	-1.2704737095578338
2216	4	-1.1779272515584438
2216	5	-1.0641491539979242
2216	6	-1.1627385569053246
2216	7	-1.1479678154393795
2216	8	-1.1903547494084599
2216	9	-1.3616196556561775
2216	10	-1.3708485106548665
2216	11	-1.2013604281619763
2216	12	-1.0784514715778357
2216	13	-1.4636560097907747
2216	14	-1.6658138923381003
2216	15	-1.602389440846487
2216	16	-1.6091045322520472
2216	17	-1.5679983448228327
2216	18	-1.7060597502837993
2216	19	-1.6307726459383574
2216	20	-1.6359270620597783
2216	21	-1.782606458453877
2216	22	-1.7179046234191517
2216	23	-1.726737109000318
2216	24	-1.6059711011412574
2216	25	-1.6966778317492341
2216	26	-1.7910835419432871
2216	27	-1.7173934167487488
2216	28	-1.776276645689741
2216	29	-1.8187525068897301
2216	30	-1.8559196685782027
2216	31	-1.7355744991883526
2216	32	-1.749394190213387
2216	33	-1.7373726744882845
2216	34	-1.6752940128804426
2216	35	-1.7748003359840396
2216	36	-1.676794182362876
2216	37	-1.8023464909095281
2216	38	-1.7453035021258287
2216	39	-1.9015790510695538
2216	40	-1.9589727196931008
2216	41	-1.856629131659254
2216	42	-1.6093500251073678
2216	43	-1.7390691450123583
2216	44	-1.8653676298547452
2216	45	-1.956683278426894
2216	46	-1.9272945378333155
2216	47	-1.81345390529345
2216	48	-2.032895108073042
2216	49	-1.9321986116632885
2216	50	-1.9811755845706145
2216	51	-2.042438217721397
2216	52	-1.9171502123792301
2216	53	-2.082693002751924
2216	54	-2.0037359936765604
2216	55	-2.065980767252025
2216	56	-1.9737808124899345
2216	57	-2.0041926127343395
2216	58	-2.018422212478786
2216	59	-1.9218667196318684
2216	60	-2.125009814712037
2216	61	-2.0353612390321807
2216	62	-2.0897320172034703
2216	63	-2.1482601779995747
2216	64	-2.0977127856245232
2216	65	-2.145150946343062
2216	66	-2.16275514758771
2216	67	-2.1606846368435733
2216	68	-2.0917046140659856
2216	69	-2.051613637101891
2216	70	-2.108972133619506
2216	71	-2.0313595664660653
2216	72	-1.9744868879599409
2216	73	-2.0236611203881303
2216	74	-2.1226485298591555
2216	75	-2.0406989560577564
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	169170.0
3	26409.0
4	18566.0
5	13561.0
6	11360.0
7	10407.0
8	9926.0
9	10520.0
10	10959.0
11	11697.0
12	12391.0
13	13115.0
14	13532.0
15	14065.0
16	15091.0
17	15493.0
18	16452.0
19	17741.0
20	19182.0
21	21947.0
22	26122.0
23	31114.0
24	34216.0
25	36644.0
26	40671.0
27	44978.0
28	52139.0
29	61801.0
30	70180.0
31	80700.0
32	97191.0
33	121935.0
34	157259.0
35	209100.0
36	293883.0
37	454712.0
38	728851.0
39	997645.0
40	173103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.527692445769226	17.50502708260296	13.397460869121646	24.569819602506165
2	16.066534951746746	19.105027899644003	39.062531562043404	25.765905586565847
3	19.57026993815756	23.374071864394388	28.45945060095405	28.596207596494
4	13.1795516530332	15.470618555833152	36.32232914712285	35.02750064401079
5	14.705913479855454	36.65962566433324	33.6866983263228	14.947762529488507
6	33.81391878253396	35.18591505333964	16.823961492245235	14.176204671881159
7	29.57072764400857	31.253877532960832	20.441271667293485	18.734123155737116
8	26.896538460151483	34.45687229313642	19.212658054387898	19.4339311923242
9	26.497513071852207	16.008795922808446	18.768065846787913	38.72562515855144
10	15.057740419159085	28.553108585268355	32.8954227997226	23.49372819584996
11	35.64234536931702	22.80859394729437	22.572411918774545	18.976648764614062
12	23.97879813783808	24.446407045352437	29.24102182239039	22.333772994419093
13	28.364326879638746	21.4517469140762	25.372627373588617	24.811298832696433
14	22.331813193606852	20.906146967795138	26.327724741007735	30.434315097590275
15	24.018911290012237	28.649445081544535	23.109929269062334	24.22171435938089
16	24.02279382088494	27.929237366203434	24.603655266068213	23.44431354684341
17	22.89183746400522	27.70197116954713	25.992402558581247	23.4137888078664
18	22.89873760486893	27.231099544379582	26.835095036753128	23.035067813998364
19	23.75259791483579	26.773191900592273	26.896703369669055	22.577506814902883
20	23.868623760530603	26.297977763212486	26.9419666748847	22.89143180137221
21	24.04854037758652	26.169619974241247	26.84863775470684	22.933201893465387
22	23.838339423419463	25.915888257363008	26.733605897862144	23.51216642135538
23	23.637392014796752	26.019682489580397	26.68438632840204	23.65853916722081
24	23.252832855567966	26.08322282227874	26.831995854903333	23.831948467249962
25	23.58584289771285	26.01374883816278	26.869255627767448	23.53115263635692
26	23.402538040536477	26.234004931737537	26.840969507427676	23.52248752029831
27	23.42360593968165	26.306442434821943	26.751786223432223	23.518165402064188
28	23.468764150484887	26.289068571475315	26.721841828316034	23.520325449723764
29	23.528285153187888	26.3153918373259	26.684436368571014	23.471886640915194
30	23.51705971073182	26.349209633418972	26.754022445386	23.379708210463207
31	23.67174155896245	26.05660489488869	26.662805780165744	23.60884776598312
32	23.422281018679904	26.36511108657249	26.622141100432	23.590466794315603
33	23.524568430160347	26.21006297721954	26.56594899167634	23.69941960094377
34	23.76544242268772	26.15532555876922	26.612549052259922	23.466682966283138
35	23.685891050276567	26.271543696637035	26.61658933217067	23.425975920915732
36	23.658985900120356	26.312452447375094	26.49092066694226	23.537640985562287
37	23.68952600299144	25.955556839089382	26.736121890789722	23.61879526712946
38	23.639495971827095	26.107176014719833	26.6443940799166	23.608933933536473
39	23.694751773322643	26.39197768178061	26.49701535195559	23.41625519294116
40	23.870086340540492	26.05348310129022	26.708292763691443	23.368137794477846
41	23.523799417337845	26.16413920654091	26.648768586583465	23.663292789537778
42	23.696573953339666	26.151292664277086	26.5719887750169	23.580144607366346
43	23.55811058209857	26.049036839119726	26.696093808098485	23.696758770683214
44	23.70104596806969	26.335107774245685	26.373318850982336	23.590527406702293
45	23.670434820688257	26.324560436837025	26.261688359498354	23.743316382976367
46	23.69193407080072	26.070627424929476	26.309774812504212	23.927663691765588
47	23.536202072540107	26.324936344579825	26.34211807409657	23.796743508783496
48	23.531384246084343	26.467893596339913	26.32062159472613	23.680100562849617
49	23.745729947302916	26.204654387531807	26.3256723151329	23.72394335003238
50	23.799163006686413	26.38849372495724	26.21691635410604	23.595426914250307
51	23.565110398900693	26.55368183710165	26.06476674520347	23.816441018794183
52	23.731027573893112	26.466922544795118	26.00690033317431	23.795149548137452
53	23.653022986029796	26.392814293433197	26.270019964307334	23.68414275622967
54	23.630558104994755	26.394227738101712	26.291282265512688	23.68393189139084
55	23.647929477508946	26.245909935682782	26.217393491079406	23.888767095728863
56	23.56158428815322	26.61290807854287	26.106724966380824	23.71878266692309
57	23.755380939729626	26.374305483360477	25.982960036655843	23.88735354025405
58	23.6145178054507	26.267342447842072	26.26250420638157	23.855635540325657
59	23.656829044053477	26.476335515468296	26.151386546034157	23.715448894444066
60	23.548247930677018	26.567694824032312	26.297434573956117	23.58662267133455
61	23.839132625268725	26.149228912779897	26.265637603569676	23.746000858381702
62	23.506097524276345	26.511152011382528	26.21406998761356	23.76868047672757
63	23.45154531836667	26.354966321635903	26.31237022819611	23.88111813180132
64	23.66284142165454	26.245579959401113	26.219483423403293	23.872095195541053
65	23.623183380338126	26.26513207576248	26.231828987316412	23.87985555658298
66	23.574258345702063	26.377069124139986	26.181271715482822	23.86740081467513
67	23.604993356108032	26.240736892969895	26.124110646623194	24.03015910429887
68	23.576746540448905	26.256651501632078	26.330258203548766	23.836343754370247
69	23.548242062831125	26.352666678861524	26.15448416224683	23.944607096060512
70	23.49363789346285	26.05075675861739	26.355448476697873	24.100156871221895
71	23.52028648809461	26.21003574843041	26.174051434221635	24.095626329253346
72	23.396858849445056	26.3890680720618	26.18731851686397	24.026754561629172
73	23.47404495509713	26.221504133595936	26.303769299400674	24.00068161190626
74	23.464453679397792	26.320563698514583	26.15006978361184	24.064912838475788
75	23.461580036164552	26.273180330931435	26.29621952572963	23.969020107174384
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1919.0
1	1747.5
2	1769.5
3	1898.0
4	1833.0
5	1519.0
6	1102.5
7	1083.5
8	1167.0
9	1515.5
10	2232.5
11	2701.5
12	2802.0
13	2838.0
14	3072.0
15	3520.5
16	3771.0
17	4120.0
18	4774.0
19	5741.0
20	6403.0
21	7513.0
22	10191.0
23	13517.0
24	15275.0
25	17957.0
26	24601.5
27	32221.5
28	35879.0
29	41510.5
30	51894.0
31	63709.5
32	70773.0
33	75820.0
34	88676.5
35	103899.0
36	111312.0
37	116543.0
38	127741.0
39	138443.0
40	143178.0
41	150589.0
42	162667.0
43	173158.0
44	178982.0
45	185942.5
46	195741.5
47	203410.5
48	208241.0
49	210935.0
50	213883.5
51	213382.0
52	212626.0
53	209896.0
54	202540.5
55	190371.5
56	182828.0
57	175783.0
58	157972.5
59	134162.0
60	121117.0
61	110112.5
62	88253.5
63	66716.0
64	56033.0
65	47448.0
66	33405.5
67	24317.5
68	20687.0
69	17474.5
70	12107.5
71	8439.0
72	6925.0
73	6266.5
74	4977.0
75	3441.5
76	2537.0
77	2101.5
78	1335.0
79	803.5
80	603.0
81	496.0
82	301.0
83	175.5
84	138.0
85	112.0
86	69.5
87	48.5
88	44.0
89	39.0
90	25.5
91	17.0
92	17.0
93	15.0
94	12.0
95	11.5
96	12.0
97	11.0
98	7.5
99	12.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18826426067551302
2	0.1285355687122523
3	0.12865565052158734
4	0.15103889978164325
5	0.15072668707737208
6	0.1270225379146305
7	0.12622999797301906
8	0.1023817506390754
9	0.12207516737002586
10	0.12526934349833854
11	0.1445304657156828
12	0.14601948015143756
13	0.16153404991752782
14	0.16220650804980416
15	0.14649980738877783
16	0.14914160719414923
17	0.13415539738913326
18	0.1330986774669847
19	0.17121264374993395
20	0.15137512884778143
21	0.16316716252448468
22	0.15670676118225826
23	0.17387845991717238
24	0.15915643009269353
25	0.18480590456666318
26	0.16998780929471632
27	0.1852381990802694
28	0.1827645138079671
29	0.18468582275732812
30	0.17625607974200663
31	0.18288459561730216
32	0.17087641468379577
33	0.18545434633707253
34	0.19959998347674307
35	0.18867253882725224
36	0.18987335692060286
37	0.20733325199792113
38	0.2004405561420885
39	0.17349419812730016
40	0.20226579964398145
41	0.19393212207612803
42	0.24583148007074257
43	0.20911046277608006
44	0.19129032227075662
45	0.18660713170668913
46	0.21778036941007167
47	0.1981590017647223
48	0.2307972375419926
49	0.2377139497596923
50	0.22657035785339838
51	0.23865058787250576
52	0.22205528182239997
53	0.21405783332068473
54	0.19765465816551497
55	0.20008031071408328
56	0.22107061098585243
57	0.20221776692024743
58	0.22637822695846227
59	0.23884271876744187
60	0.24146050221094628
61	0.22813142137475417
62	0.24239714032375978
63	0.26093777168509363
64	0.24064394590746782
65	0.26562096224916115
66	0.22897199404009963
67	0.24950598343639555
68	0.22371241079122386
69	0.21787643485753974
70	0.22731486507127577
71	0.22191118365119789
72	0.22027807104424102
73	0.21545078230897144
74	0.21232865526625977
75	0.24105222405920707
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	4163828.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.316908081533235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.20810737615444	43.10696177465125
2	12.82817012309442	14.705420955993475
3	4.895813536968747	8.418386834482915
4	2.4146081107382873	5.535914845444442
5	1.3502574078454244	3.8696289865942757
6	0.8131432348506914	2.7964113629434597
7	0.5289824766539503	2.122374799378139
8	0.3701043461104992	1.6970589429273157
9	0.26760431660222006	1.3804426815219868
>10	1.2456510135815417	12.344728741028439
>50	0.06074069242234711	2.3499348303466623
>100	0.016396788270698757	1.5309669478015508
>500	4.205767067347154E-4	0.14176829688615808
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.401636186701276E-5	0.0
3	0.0	0.0	0.0	2.401636186701276E-5	0.0
4	0.0	0.0	0.0	4.803272373402552E-5	0.0
5	0.0	0.0	0.0	4.803272373402552E-5	0.0
6	0.0	0.0	0.0	4.803272373402552E-5	0.0
7	0.0	0.0	0.0	4.803272373402552E-5	0.0
8	0.0	0.0	0.0	4.803272373402552E-5	0.0
9	0.0	0.0	0.0	4.803272373402552E-5	0.0
10	0.0	0.0	0.0	7.204908560103828E-5	0.0
11	0.0	0.0	0.0	7.204908560103828E-5	0.0
12	0.0	0.0	0.0	7.204908560103828E-5	0.0
13	0.0	0.0	0.0	7.204908560103828E-5	0.0
14	0.0	0.0	0.0	7.204908560103828E-5	0.0
15	0.0	0.0	0.0	9.606544746805104E-5	0.0
16	0.0	0.0	0.0	9.606544746805104E-5	0.0
17	0.0	0.0	0.0	9.606544746805104E-5	0.0
18	0.0	0.0	0.0	9.606544746805104E-5	0.0
19	0.0	0.0	0.0	9.606544746805104E-5	0.0
20	0.0	0.0	0.0	9.606544746805104E-5	0.0
21	0.0	0.0	0.0	1.9213089493610208E-4	0.0
22	0.0	0.0	0.0	2.1614725680311483E-4	0.0
23	0.0	0.0	0.0	2.1614725680311483E-4	0.0
24	0.0	0.0	0.0	3.362290661381786E-4	0.0
25	0.0	0.0	0.0	3.602454280051914E-4	0.0
26	0.0	0.0	0.0	5.283599610742807E-4	0.0
27	2.401636186701276E-5	0.0	0.0	6.484417704093444E-4	0.0
28	2.401636186701276E-5	0.0	0.0	0.0012488508170846635	0.0
29	2.401636186701276E-5	0.0	0.0	0.0022575380154991995	0.0
30	2.401636186701276E-5	0.0	0.0	0.0035544215563178883	0.0
31	2.401636186701276E-5	0.0	0.0	0.007613186711843045	0.0
32	2.401636186701276E-5	0.0	0.0	0.012176295466575468	0.0
33	2.401636186701276E-5	0.0	0.0	0.018156369571461644	0.0
34	2.401636186701276E-5	0.0	0.0	0.026514063501182086	0.0
35	2.401636186701276E-5	0.0	0.0	0.03676905001839653	0.0
36	2.401636186701276E-5	0.0	0.0	0.049665836340982386	0.0
37	2.401636186701276E-5	0.0	0.0	0.06726982958950274	0.0
38	2.401636186701276E-5	0.0	0.0	0.08780381898579864	2.401636186701276E-5
39	2.401636186701276E-5	0.0	0.0	0.10869805381009974	2.401636186701276E-5
40	2.401636186701276E-5	0.0	0.0	0.12827138873171515	2.401636186701276E-5
41	2.401636186701276E-5	0.0	0.0	0.14791677273893158	2.401636186701276E-5
42	2.401636186701276E-5	0.0	0.0	0.16785035308855217	2.401636186701276E-5
43	2.401636186701276E-5	0.0	0.0	0.19056983141474623	2.401636186701276E-5
44	2.401636186701276E-5	0.0	0.0	0.2166996331260561	2.401636186701276E-5
45	2.401636186701276E-5	0.0	0.0	0.24575943098514155	2.401636186701276E-5
46	2.401636186701276E-5	0.0	0.0	0.2779893886106727	2.401636186701276E-5
47	4.803272373402552E-5	0.0	0.0	0.3126449987847721	2.401636186701276E-5
48	4.803272373402552E-5	0.0	0.0	0.3484533943284881	2.401636186701276E-5
49	4.803272373402552E-5	0.0	0.0	0.3845259698527413	2.401636186701276E-5
50	4.803272373402552E-5	0.0	0.0	0.42158321621354194	2.401636186701276E-5
51	4.803272373402552E-5	0.0	0.0	0.45979324794395926	2.401636186701276E-5
52	4.803272373402552E-5	0.0	0.0	0.4975709851607703	2.401636186701276E-5
53	4.803272373402552E-5	0.0	0.0	0.5359251150623897	2.401636186701276E-5
54	4.803272373402552E-5	0.0	0.0	0.5773533392829867	2.401636186701276E-5
55	4.803272373402552E-5	0.0	0.0	0.6248096703322039	2.401636186701276E-5
56	4.803272373402552E-5	0.0	0.0	0.6778618136964351	2.401636186701276E-5
57	4.803272373402552E-5	0.0	0.0	0.7334596914185696	2.401636186701276E-5
58	4.803272373402552E-5	0.0	0.0	0.7928521543156922	2.401636186701276E-5
59	4.803272373402552E-5	0.0	0.0	0.8537336316485695	2.401636186701276E-5
60	4.803272373402552E-5	0.0	0.0	0.9178573178334936	2.401636186701276E-5
61	4.803272373402552E-5	0.0	0.0	0.9820770694658857	2.401636186701276E-5
62	4.803272373402552E-5	0.0	0.0	1.0460566574796077	2.401636186701276E-5
63	4.803272373402552E-5	0.0	0.0	1.114407223353126	2.401636186701276E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3395	0.0	35.598377	1
GTATCAA	6735	0.0	34.50479	1
TATCAAC	8925	0.0	25.631418	2
TCAACGC	8910	0.0	25.519976	4
ATCAACG	8985	0.0	25.268553	3
CAACGCA	9130	0.0	24.904438	5
AACGCAG	9585	0.0	23.632315	6
GTACATG	11695	0.0	20.579483	1
ACGCAGA	11245	0.0	19.961884	7
TACATGG	11810	0.0	19.749866	2
TGCGATA	230	3.4742698E-10	19.493721	13
CGCAGAG	11470	0.0	19.420929	8
AGAGTAC	11350	0.0	18.685566	11
GTGGTAT	2020	0.0	18.29087	1
ACATGGG	12440	0.0	18.00101	3
GAGTACT	6285	0.0	17.942375	12
CAGAGTA	12225	0.0	17.572975	10
GCAGAGT	12860	0.0	16.892756	9
TGGTATC	2035	0.0	16.785648	2
AGTACTT	6850	0.0	16.615088	13
>>END_MODULE