FastQCFastQC Report
Mon 6 Feb 2017
SRR2077270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4163828
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40250.037.814551
GTATCAA81650.034.8670921
ATCAACG110450.025.5132163
TATCAAC111400.025.5089172
TCAACGC111350.025.3374444
CAACGCA114550.024.5710475
AACGCAG120250.023.4868556
GTGGTAT21000.020.9197541
ACGCAGA141950.019.7502277
GTACATG116500.019.5376381
TACATGG116700.019.344062
CGCAGAG148000.018.8966668
TGGTATC22950.018.8292352
AGAGTAC144550.018.41678611
GAGTACT94500.017.83058512
ACATGGG120800.017.8031483
GTACCGT3350.017.5210766
CAGAGTA155700.017.45253210
AGTACTT97800.017.36347813
GCAGAGT162450.016.8973969
Produced by FastQC (version 0.11.5)