FastQCFastQC Report
Mon 6 Feb 2017
SRR2077269_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077269_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4552012
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52400.038.9400141
GTATCAA106550.035.384181
TCAACGC138900.026.6006534
ATCAACG140300.026.4081133
TATCAAC145550.025.7868272
CAACGCA144250.025.6137985
AACGCAG148050.025.00596
GTGGTAT23450.021.4956191
ACGCAGA173250.021.1905447
GTACATG180400.020.7650571
GAGTACT92950.020.51201812
CGCAGAG178300.020.4181588
AGAGTAC175150.019.86113711
TACATGG184850.019.856532
CAGAGTA184000.019.13049510
AGTACTT103400.019.04148313
GCAGAGT189700.018.8099029
ACATGGG192450.018.7322163
GTACTTT103800.018.27130914
TGGTATC27550.016.904152
Produced by FastQC (version 0.11.5)