FastQCFastQC Report
Mon 6 Feb 2017
SRR2077269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4552012
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50820.11164293942986091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64900.041.738881
GTATCAA135700.036.584391
ATCAACG189600.026.0625383
TATCAAC190350.026.0290722
TCAACGC190350.025.923234
CAACGCA195400.025.2348185
AACGCAG200800.024.532526
GTGGTAT24650.023.4381481
ACGCAGA234550.020.8854187
GTACATG175650.020.7200761
CGCAGAG240300.020.3724178
GAGTACT158100.020.24356812
TACATGG180000.020.034442
AGAGTAC236950.019.97652411
AGTACTT166250.019.5346113
CAGAGTA246600.019.34880810
GCAGAGT254550.018.8930859
GTACTTT170100.018.73930714
ACATGGG186450.018.6020743
TGGTATC30800.018.2979432
Produced by FastQC (version 0.11.5)