FastQCFastQC Report
Mon 6 Feb 2017
SRR2077268_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077268_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3879457
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39600.039.6705931
GTATCAA82800.036.1527441
TCAACGC110900.026.5365434
ATCAACG111450.026.4052493
TATCAAC113800.025.9805682
CAACGCA114750.025.7968755
AACGCAG117700.025.2473356
ACGCAGA139250.021.071067
CGCAGAG145150.020.1216358
GTACATG125500.019.835541
AGAGTAC140450.019.59373711
GAGTACT80750.019.38898712
TACATGG128150.019.1139222
CAGAGTA149600.018.6251210
GCAGAGT154350.018.2530219
GTGGTAT19800.017.9607521
ACATGGG133400.017.7931943
AGTACTT88300.017.77204313
GTACTTT89550.017.36968614
CGATCGT1653.8379818E-516.73103142
Produced by FastQC (version 0.11.5)