FastQCFastQC Report
Mon 6 Feb 2017
SRR2077268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3879457
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41410.10674174246550483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49850.042.1302071
GTATCAA104600.036.1541821
GTGGTAT20050.026.7477571
TATCAAC146650.025.5582372
ATCAACG148050.025.3902263
TCAACGC149050.025.1734754
CAACGCA153550.024.3569985
AACGCAG158150.023.6242686
GTACATG119750.020.427441
ACGCAGA183650.020.2118767
GAGTACT130300.020.11500212
TGGTATC24500.019.899252
TACATGG123750.019.5306032
CGCAGAG190050.019.4406438
AGTACTT135400.019.42357813
AGAGTAC186800.019.05919311
GTACTTT138450.018.88695314
CAGAGTA195700.018.33358810
ACATGGG127350.018.2751753
GCAGAGT202350.017.8666749
Produced by FastQC (version 0.11.5)