FastQCFastQC Report
Mon 6 Feb 2017
SRR2077267_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077267_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2783004
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58650.034.4913671
GGTATCA29100.033.5717741
TATCAAC77750.025.781382
ATCAACG78100.025.4454253
TCAACGC78150.025.4291464
CAACGCA80550.024.5434255
AACGCAG83200.023.7808866
CGCGATC953.0729463E-421.78337323
GTACATG97600.021.2218041
TAATTCG2201.891749E-1020.3960859
TACATGG98050.020.3029272
ACGCAGA97100.020.1650757
CGCAGAG99100.019.621448
GAGTACT54350.019.22309312
ACATGGG100850.018.8501133
AGAGTAC98000.018.68308811
AGTACTT57150.018.16388113
CAGAGTA104000.017.70372410
GTGGTAT15600.017.4816861
GTACTTT58650.017.34683214
Produced by FastQC (version 0.11.5)