FastQCFastQC Report
Mon 6 Feb 2017
SRR2077267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2783004
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36450.039.090031
GTATCAA78650.036.8038331
TATCAAC106500.027.098972
ATCAACG106200.027.049243
TCAACGC108850.026.3599054
CAACGCA111600.025.6488845
AACGCAG114500.025.086576
ACGCAGA133350.021.4113357
GAGTACT91300.020.95272812
CGCAGAG136050.020.936798
AGAGTAC134500.020.51285611
CGTAGAC1206.294691E-520.1648653
CGCTATC4800.020.1184833
AGTACTT95000.020.09020613
CAGAGTA141050.019.75578110
GTACTTT97500.019.60236214
GTGGTAT16100.019.5470771
GCAGAGT145700.019.1949969
GTACATG93250.018.9512731
TATCTCG5150.018.7505336
Produced by FastQC (version 0.11.5)