FastQCFastQC Report
Mon 6 Feb 2017
SRR2077266_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077266_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3276788
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107300.033.014221
GGTATCA56650.029.864111
ATCAACG139650.025.1486513
TCAACGC142050.024.7476584
GTATACG1551.5133992E-924.5027281
TATCAAC144400.024.4636172
CAACGCA143950.024.4232585
AACGCAG148600.023.497056
TTAGTCG2057.2759576E-1121.88484639
GTACATG147950.021.4697171
TAGTCGG2101.0186341E-1021.36377740
GCGCGAC1304.9550017E-621.2165879
TACATGG150600.020.6847692
AGAGTAC156050.020.61357911
ACGCAGA168650.020.5419757
GAGTACT89550.020.25173812
CGCAGAG171800.020.0069548
GTACTTT93450.019.26192914
AGTACTT95200.019.19674513
ACATGGG154500.019.1140673
Produced by FastQC (version 0.11.5)