FastQCFastQC Report
Mon 6 Feb 2017
SRR2077266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3276788
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53190.16232359249362485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36800.11230509877355507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65850.035.9789851
GTATCAA132050.034.8359261
ATCAACG185350.024.7551843
TATCAAC188350.024.3766332
TCAACGC189200.024.233914
CAACGCA191600.023.931295
AACGCAG197750.023.1863216
GTACATG137300.021.5382121
TACATGG140150.020.4751132
ACGCAGA225000.020.2726447
GAGTACT155550.020.26830512
AGAGTAC217750.020.01326211
AGTACTT159750.019.92114413
CGCAGAG228000.019.9153358
GTACTTT162850.019.21343414
ACATGGG144300.019.1934933
GCAGAGT238500.018.7055669
CAGAGTA238350.018.60229510
TACACCG2053.794412E-818.5268845
TACTTTT177600.017.65468415
Produced by FastQC (version 0.11.5)