##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077266_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3276788 Sequences flagged as poor quality 0 Sequence length 75 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.857335903329723 31.0 31.0 34.0 27.0 34.0 2 30.937511978193278 33.0 31.0 34.0 27.0 34.0 3 31.008762239119527 33.0 31.0 34.0 27.0 34.0 4 34.34528385724069 37.0 35.0 37.0 31.0 37.0 5 34.07024653410596 37.0 35.0 37.0 30.0 37.0 6 34.04246780688894 37.0 35.0 37.0 30.0 37.0 7 33.861533611573286 37.0 35.0 37.0 29.0 37.0 8 33.815707943266396 37.0 35.0 37.0 29.0 37.0 9 35.2487109327793 39.0 35.0 39.0 28.0 39.0 10 35.15288141924348 39.0 35.0 39.0 28.0 39.0 11 35.12767624881439 39.0 35.0 39.0 27.0 39.0 12 35.031596490221524 39.0 35.0 39.0 27.0 39.0 13 34.94247018726875 39.0 35.0 39.0 27.0 39.0 14 36.16478087688309 40.0 36.0 41.0 27.0 41.0 15 36.217176393468236 40.0 36.0 41.0 27.0 41.0 16 36.2199522825401 40.0 36.0 41.0 27.0 41.0 17 36.24724455778036 40.0 36.0 41.0 27.0 41.0 18 36.26633734010257 40.0 36.0 41.0 27.0 41.0 19 36.325100983035824 40.0 37.0 41.0 27.0 41.0 20 36.300353577955 40.0 37.0 41.0 27.0 41.0 21 36.28565564815301 40.0 37.0 41.0 27.0 41.0 22 36.26420110181068 40.0 37.0 41.0 27.0 41.0 23 36.23653498486933 40.0 36.0 41.0 27.0 41.0 24 36.22418874824981 40.0 36.0 41.0 27.0 41.0 25 36.202491891449796 40.0 36.0 41.0 27.0 41.0 26 36.122038410785194 40.0 36.0 41.0 27.0 41.0 27 36.070136975599276 40.0 36.0 41.0 27.0 41.0 28 36.04870928482404 40.0 36.0 41.0 27.0 41.0 29 35.98352502511606 40.0 36.0 41.0 27.0 41.0 30 35.88843037755265 40.0 36.0 41.0 27.0 41.0 31 35.76583349304258 40.0 35.0 41.0 27.0 41.0 32 35.6716525451143 40.0 35.0 41.0 27.0 41.0 33 35.59239963036974 40.0 35.0 41.0 26.0 41.0 34 35.50934238040423 40.0 35.0 41.0 26.0 41.0 35 35.414360953470286 40.0 35.0 41.0 25.0 41.0 36 35.34800206787867 40.0 35.0 41.0 25.0 41.0 37 35.27400552003974 40.0 35.0 41.0 25.0 41.0 38 35.199877135780525 40.0 35.0 41.0 25.0 41.0 39 35.113077806681424 40.0 35.0 41.0 24.0 41.0 40 35.03452832468869 40.0 35.0 41.0 24.0 41.0 41 34.96708789216758 40.0 35.0 41.0 24.0 41.0 42 34.873758998140865 39.0 35.0 41.0 23.0 41.0 43 34.76141361601666 39.0 35.0 41.0 22.0 41.0 44 34.60712411056193 39.0 35.0 41.0 21.0 41.0 45 34.47657645230635 39.0 34.0 41.0 20.0 41.0 46 34.297509024080895 39.0 34.0 41.0 18.0 41.0 47 34.099864257315396 39.0 34.0 41.0 15.0 41.0 48 33.96428636823621 39.0 33.0 41.0 12.0 41.0 49 33.8547342702671 39.0 33.0 41.0 9.0 41.0 50 33.836449596373036 39.0 33.0 41.0 8.0 41.0 51 33.75660372291402 39.0 33.0 41.0 8.0 41.0 52 33.69101357793058 39.0 33.0 41.0 7.0 41.0 53 33.59728734358158 39.0 33.0 41.0 6.0 41.0 54 33.56105246967457 39.0 33.0 41.0 2.0 41.0 55 33.48203545667282 39.0 33.0 41.0 2.0 41.0 56 33.35943491003995 39.0 33.0 41.0 2.0 41.0 57 33.28442364901239 38.0 33.0 41.0 2.0 41.0 58 33.191513762867785 38.0 33.0 41.0 2.0 41.0 59 33.10691750580141 38.0 33.0 41.0 2.0 41.0 60 33.070697890739346 38.0 33.0 41.0 2.0 41.0 61 32.95961655133014 38.0 33.0 41.0 2.0 41.0 62 32.81892969578746 37.0 33.0 40.0 2.0 41.0 63 32.689713219164624 37.0 33.0 40.0 2.0 41.0 64 32.5477467568851 37.0 33.0 40.0 2.0 41.0 65 32.42430636342662 37.0 33.0 40.0 2.0 41.0 66 32.23279137985124 37.0 32.0 40.0 2.0 41.0 67 32.050649294369975 36.0 32.0 40.0 2.0 41.0 68 31.843754310623694 36.0 32.0 39.0 2.0 41.0 69 31.6825556001792 36.0 32.0 39.0 2.0 41.0 70 31.500540773464746 36.0 32.0 39.0 2.0 41.0 71 31.330639028219096 35.0 32.0 39.0 2.0 41.0 72 31.134958074797638 35.0 32.0 39.0 2.0 41.0 73 30.939448630793326 35.0 32.0 38.0 2.0 40.0 74 30.681916254576127 35.0 31.0 37.0 2.0 40.0 75 30.465995053692822 35.0 31.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42688412809502196 1101 2 0.4480256002434331 1101 3 0.4960902069365929 1101 4 0.3915178095610514 1101 5 0.4794736755585163 1101 6 0.5428639107999444 1101 7 0.5388591748924938 1101 8 0.5812248810654381 1101 9 0.741977426238364 1101 10 0.6394005120276418 1101 11 0.6913739269704564 1101 12 0.6725643108883688 1101 13 0.7097860003599124 1101 14 0.8548717317670764 1101 15 0.8351582132208577 1101 16 0.7891843414081308 1101 17 0.8920035836819977 1101 18 0.7459469287342486 1101 19 0.7355569139241069 1101 20 0.7454724088132423 1101 21 0.6753877651372946 1101 22 0.8198614160636097 1101 23 0.7509856003246469 1101 24 0.8310592815794706 1101 25 0.8070387087927671 1101 26 0.9216551511405555 1101 27 0.9280842948731731 1101 28 0.7824046784292733 1101 29 0.8270033471594118 1101 30 0.8022947756238921 1101 31 0.8532277642365358 1101 32 0.7897789354486804 1101 33 0.8514937879438023 1101 34 0.8044507876460898 1101 35 0.9834815207555749 1101 36 0.8509027986401847 1101 37 0.9198775851995222 1101 38 1.0363488292491851 1101 39 0.9636397366351943 1101 40 1.0264065564603087 1101 41 0.9512460782850738 1101 42 1.005349529661288 1101 43 1.0673492465512382 1101 44 1.1282623842932153 1101 45 1.136591167094899 1101 46 1.1098111122405214 1101 47 1.0783223599533045 1101 48 1.0743209012847146 1101 49 1.0096635616811227 1101 50 0.8885603392783779 1101 51 0.9066603079037421 1101 52 0.9336386652549677 1101 53 0.9146185555785422 1101 54 0.8882262591926775 1101 55 0.9027853635787153 1101 56 0.9296917851029249 1101 57 0.865813767431014 1101 58 0.9006785831072932 1101 59 0.9442008219461258 1101 60 0.8914807997371597 1101 61 0.9313559103029618 1101 62 0.9459655441893702 1101 63 0.9329934168276495 1101 64 0.952713752161884 1101 65 0.8228080878086672 1101 66 0.8670374320294343 1101 67 0.8760824749204019 1101 68 0.9186570084571137 1101 69 0.9242710976366055 1101 70 0.8670045399740332 1101 71 0.8942181024655547 1101 72 0.9372589661366462 1101 73 0.8963882640677667 1101 74 0.9701353810842335 1101 75 0.9169995885085207 1102 1 0.5110885608057849 1102 2 0.5921427444802738 1102 3 0.5612698727700618 1102 4 0.5514220335658422 1102 5 0.582268633245441 1102 6 0.5933564409348762 1102 7 0.743083542396846 1102 8 0.7366316376643596 1102 9 0.7760518983133267 1102 10 0.7843977469334575 1102 11 0.7874129627977098 1102 12 0.7947793459882533 1102 13 0.8250254052855297 1102 14 0.95583246597878 1102 15 0.8271706577164579 1102 16 0.773901676723014 1102 17 0.8968795800287381 1102 18 0.8626565851395611 1102 19 0.8898802178767511 1102 20 0.8855455755993233 1102 21 0.8761585094493824 1102 22 0.9548033805209712 1102 23 0.9640052161712163 1102 24 1.0120960794577343 1102 25 1.0390823177255086 1102 26 1.0080324860250158 1102 27 1.0618866584664985 1102 28 1.0521742797068043 1102 29 1.0144483928771706 1102 30 1.0245934043451257 1102 31 1.0840925991300665 1102 32 1.0733391386945854 1102 33 1.134590828845674 1102 34 1.1249140080244615 1102 35 1.1452274159750715 1102 36 1.087398559836636 1102 37 1.1139238912779206 1102 38 1.1400702567100183 1102 39 1.2024148446455527 1102 40 1.1918621997309984 1102 41 1.2186031439530964 1102 42 1.1552921119424866 1102 43 1.1352515447833937 1102 44 1.2730494381875275 1102 45 1.2935344310377062 1102 46 1.2901042484957088 1102 47 1.2878639781874952 1102 48 1.2569536433368498 1102 49 1.2062989728289821 1102 50 1.2805557120075122 1102 51 1.1806921924502376 1102 52 1.2118328205688726 1102 53 1.2252179220854273 1102 54 1.2403899121444653 1102 55 1.2609176059310627 1102 56 1.2439539885746882 1102 57 1.2446741810755597 1102 58 1.240848135249088 1102 59 1.2785771450987085 1102 60 1.2394413160782989 1102 61 1.2963298686048077 1102 62 1.2708150962247657 1102 63 1.3033609893699563 1102 64 1.2977377312231013 1102 65 1.2900213734152075 1102 66 1.31502084775137 1102 67 1.3335178458311248 1102 68 1.4317394222888247 1102 69 1.393173037264937 1102 70 1.3637300736057725 1102 71 1.3212481253189914 1102 72 1.3625820541532079 1102 73 1.3592745097614376 1102 74 1.3454653727683947 1102 75 1.4015973005532807 1103 1 0.5323758835707864 1103 2 0.5140179631457578 1103 3 0.5418824866738383 1103 4 0.5169371277350763 1103 5 0.5309345133887433 1103 6 0.680071393954762 1103 7 0.5966248143829986 1103 8 0.5614896051018192 1103 9 0.7544571048117206 1103 10 0.7730944479980977 1103 11 0.8389202144346299 1103 12 0.7820052018657577 1103 13 0.8003563146827659 1103 14 0.9374634940747697 1103 15 0.8708686540784996 1103 16 0.8408253150778009 1103 17 0.8508128054077062 1103 18 0.857073153604766 1103 19 0.823158201919 1103 20 0.9616972107761086 1103 21 0.8276666135255581 1103 22 0.8398771554916848 1103 23 0.8897526059634657 1103 24 0.9283296089751758 1103 25 0.9644353062111577 1103 26 0.9357579907385727 1103 27 0.9054831097117386 1103 28 0.9649898924918787 1103 29 0.9089241625648583 1103 30 0.8527187213032406 1103 31 0.9265530269168991 1103 32 0.895388843014409 1103 33 0.8803464904896501 1103 34 0.9471898594642596 1103 35 0.9745188880679194 1103 36 0.9620549800922902 1103 37 0.98711160884082 1103 38 0.9477395843091259 1103 39 0.9561605060374418 1103 40 0.9694583365309981 1103 41 0.8474687337473839 1103 42 0.8704393694562285 1103 43 0.9435989034775716 1103 44 0.978974169077695 1103 45 0.8968694651468994 1103 46 0.9103190385720552 1103 47 0.9679751162089474 1103 48 0.9492634150212567 1103 49 0.9362479946822901 1103 50 0.9123742972528248 1103 51 0.890193339072205 1103 52 0.8922625976487097 1103 53 0.9020806283557832 1103 54 0.9054917829660027 1103 55 0.9113348658293532 1103 56 0.9369643559226688 1103 57 0.8981364725134213 1103 58 0.9753006347126671 1103 59 0.8885953941365088 1103 60 0.8845197078964873 1103 61 0.8975752869147584 1103 62 1.006217814740161 1103 63 1.0249086981386029 1103 64 1.016300899729778 1103 65 1.0037583681893878 1103 66 1.0219957655696135 1103 67 0.9452282551674429 1103 68 0.9740871229615742 1103 69 1.0149337965868312 1103 70 0.9983384600490339 1103 71 0.9889716343218247 1103 72 0.9980500366943375 1103 73 1.0292825103824903 1103 74 1.0237935451974387 1103 75 1.0124832142316365 1104 1 0.4769142878002661 1104 2 0.5436996847007904 1104 3 0.5497641127040644 1104 4 0.5361118617801637 1104 5 0.5727739620641401 1104 6 0.6013964224066868 1104 7 0.5850940724721667 1104 8 0.6478882455182742 1104 9 0.6817376855146122 1104 10 0.8703319168681034 1104 11 0.853709848220987 1104 12 0.7646624218214555 1104 13 0.6940487448606802 1104 14 0.9446340510692437 1104 15 0.9469446630647766 1104 16 0.9324842344256581 1104 17 0.9302744210755876 1104 18 0.8554165669612956 1104 19 0.844861472321675 1104 20 0.9683592504324281 1104 21 0.9180474761688941 1104 22 0.9631805668514914 1104 23 0.9751238266747393 1104 24 1.012468589285774 1104 25 1.0662087637005655 1104 26 1.0944700307308821 1104 27 1.1156534041133384 1104 28 1.0899424289828161 1104 29 1.0310763192953658 1104 30 0.9876838219337358 1104 31 0.9927250345864636 1104 32 1.110133611730653 1104 33 1.0947342411124623 1104 34 1.176521928442149 1104 35 1.133405160918585 1104 36 1.2023088704855525 1104 37 1.104154680442626 1104 38 1.1385910632986835 1104 39 1.1343805173248356 1104 40 1.2417461575189321 1104 41 1.2115867834249983 1104 42 1.1347571171302206 1104 43 1.1625243881027956 1104 44 1.151626364948413 1104 45 1.133158238066727 1104 46 1.180777523633715 1104 47 1.221403288418557 1104 48 1.2435584648619766 1104 49 1.2203584292538565 1104 50 1.1866439133724143 1104 51 1.1839325280607937 1104 52 1.2197176119289992 1104 53 1.1193828631529072 1104 54 1.2123700406688371 1104 55 1.1707908927808575 1104 56 1.2217955900402302 1104 57 1.1817979945764137 1104 58 1.2120256224797146 1104 59 1.2083935440828881 1104 60 1.1561900560122496 1104 61 1.1032039202021977 1104 62 1.0812500846901756 1104 63 1.070234287556623 1104 64 1.0858959089118017 1104 65 1.0570255811783937 1104 66 1.140125207187559 1104 67 1.1128749543907333 1104 68 1.1541042678588163 1104 69 1.1145691162620182 1104 70 1.0838213416379787 1104 71 1.1690066257602858 1104 72 1.1393704695528157 1104 73 1.1627902598393938 1104 74 1.2160414614937558 1104 75 1.2077402580956402 1105 1 0.4619600034246538 1105 2 0.500050194659174 1105 3 0.4181995619216039 1105 4 0.4529707159804204 1105 5 0.46265902717954077 1105 6 0.521605873120734 1105 7 0.5705520959410464 1105 8 0.5960996231762223 1105 9 0.6655377050981883 1105 10 0.6876589054785498 1105 11 0.6321830676777083 1105 12 0.7980796593137853 1105 13 0.7747500475028275 1105 14 0.907151884323433 1105 15 0.7645365813965768 1105 16 0.8331355889175001 1105 17 0.8446453921971653 1105 18 0.7896630319750315 1105 19 0.8219897912118412 1105 20 0.9026088902583425 1105 21 0.8913355255408959 1105 22 0.8980231446251992 1105 23 0.8496253393891919 1105 24 0.8574630634416351 1105 25 0.8281849907069869 1105 26 0.8366682639980354 1105 27 0.8613060836715292 1105 28 0.8272680774883341 1105 29 0.8362817914684726 1105 30 0.9007945597166369 1105 31 0.8508880448163794 1105 32 0.8243356828444419 1105 33 0.9120182026631483 1105 34 0.9711245305182103 1105 35 0.8488815013827704 1105 36 0.9781080626128187 1105 37 0.9191587091121818 1105 38 0.9363891138303302 1105 39 0.9151982520261654 1105 40 0.9575649599597256 1105 41 1.031668088693884 1105 42 1.0150388766980214 1105 43 1.0318475315144084 1105 44 0.9935064319068729 1105 45 1.0394549934420994 1105 46 1.0272717623521856 1105 47 1.03674785746788 1105 48 0.9755600368952884 1105 49 0.9375847596087752 1105 50 0.8001464563969662 1105 51 0.8667660345606336 1105 52 0.810163165069838 1105 53 0.8739185000853951 1105 54 0.8797625139578003 1105 55 0.7940345114919296 1105 56 0.8815881478126215 1105 57 0.833181310370307 1105 58 0.8553944591249305 1105 59 0.8421173848837711 1105 60 0.8509127328267354 1105 61 0.8591007160814925 1105 62 0.8360826501088496 1105 63 0.8864152525190647 1105 64 0.798826969035062 1105 65 0.7456965646984415 1105 66 0.7643772465030949 1105 67 0.7608528663869478 1105 68 0.7065217536548793 1105 69 0.7557342155183449 1105 70 0.790230502073932 1105 71 0.7906716108904668 1105 72 0.819366863634567 1105 73 0.8194269707607127 1105 74 0.9242436388802027 1105 75 0.8794099553154737 1106 1 0.17337862409554816 1106 2 0.1742435097502657 1106 3 0.1800598014206578 1106 4 0.2624727529619264 1106 5 0.17202462199100665 1106 6 0.23226401580993894 1106 7 0.25159121957070596 1106 8 0.24857649690844852 1106 9 0.33726185914940743 1106 10 0.4005221289018053 1106 11 0.3462742543426174 1106 12 0.2993895139193512 1106 13 0.23145052839367963 1106 14 0.2694692494787745 1106 15 0.227265586055303 1106 16 0.1949223908801585 1106 17 0.3467169057140822 1106 18 0.34224901546384245 1106 19 0.34809240895012294 1106 20 0.39307194662485045 1106 21 0.32989269173972957 1106 22 0.3775379649667059 1106 23 0.3233171584460095 1106 24 0.4214570718117372 1106 25 0.3814456922508782 1106 26 0.4741516882249428 1106 27 0.44489191877041634 1106 28 0.3902848054791832 1106 29 0.36481797946986916 1106 30 0.3119451582199346 1106 31 0.3785965232886781 1106 32 0.4213529639990341 1106 33 0.40500319540898744 1106 34 0.4643733185178789 1106 35 0.5087959923019056 1106 36 0.5103095305165368 1106 37 0.49329796205770293 1106 38 0.49917409455511574 1106 39 0.52485323161563 1106 40 0.47747954698211004 1106 41 0.4113290348286682 1106 42 0.49762281107025785 1106 43 0.44179345959562966 1106 44 0.5071716205925583 1106 45 0.467321121952331 1106 46 0.484748154753774 1106 47 0.513575574163994 1106 48 0.4682996813230673 1106 49 0.5540632729600361 1106 50 0.3957116012823789 1106 51 0.43197261336504766 1106 52 0.457102578165717 1106 53 0.42019119287053286 1106 54 0.5059191123982245 1106 55 0.47129861020396646 1106 56 0.5015323639530465 1106 57 0.402486692428738 1106 58 0.4817738053329492 1106 59 0.4691837471040543 1106 60 0.3789310220880395 1106 61 0.5299017903528451 1106 62 0.48578718965134016 1106 63 0.44085492908441637 1106 64 0.43496604736588296 1106 65 0.41498906240703093 1106 66 0.38584331448457476 1106 67 0.4122802601435964 1106 68 0.4067194657136035 1106 69 0.38255331550008265 1106 70 0.4104274371623866 1106 71 0.3896297703081544 1106 72 0.4045194259017286 1106 73 0.3934421853698389 1106 74 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73165.0 3 28761.0 4 16316.0 5 10462.0 6 7736.0 7 6938.0 8 6992.0 9 7212.0 10 8089.0 11 8655.0 12 9345.0 13 9702.0 14 10383.0 15 11184.0 16 12093.0 17 13344.0 18 14390.0 19 15969.0 20 18815.0 21 21910.0 22 28582.0 23 35009.0 24 35212.0 25 34397.0 26 34091.0 27 33683.0 28 38202.0 29 46032.0 30 51874.0 31 57322.0 32 67983.0 33 84482.0 34 110401.0 35 151113.0 36 209181.0 37 313647.0 38 522953.0 39 862220.0 40 248943.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.69966087532281 18.812318435139964 14.627408750296244 24.860611939240975 2 14.705398476728956 20.308530604757465 39.1482123251692 25.837858593344382 3 17.777932404231798 25.470089852211697 29.591383318776067 27.160594424780438 4 11.644294009897632 16.427721436128373 37.338103070074915 34.589881483899084 5 12.853245844750532 38.64087219763133 33.91022645002245 14.595655507595684 6 31.32720677382649 37.884266416164245 16.935818323852967 13.8527084861563 7 27.572341328351328 33.16113059750472 20.909154738246347 18.357373335897602 8 26.507986221767993 34.8165018871196 19.274475471108328 19.40103642000408 9 26.51702306636561 15.763690740630482 19.438218199971605 38.28106799303231 10 14.23507827191247 30.13328204752485 33.065059711334165 22.566579969228513 11 34.34822554109779 23.85182878997378 23.033199947500314 18.766745721428112 12 23.43540532448567 26.223855699961508 29.947971086221965 20.392767889330866 13 27.97031230240936 22.6411457706516 26.170362763431278 23.218179163507763 14 21.829451939353525 21.709150709960454 28.33515311842771 28.12624423225832 15 23.156605014129596 29.587662410999414 24.927978432905935 22.327754141965055 16 22.24447037971082 29.011041076394285 26.270477827942134 22.474010715952765 17 21.030447995916987 28.279688841188616 27.748524730678643 22.941338432215755 18 20.93761646719902 27.147521608528578 29.883945634752145 22.03091628952025 19 21.82259371570981 26.918610063593757 30.420158320526575 20.838637900169857 20 22.322561977442327 26.143442338995083 30.189484338034855 21.34451134552773 21 22.76288769312346 25.871820146351187 30.124802377664945 21.240489782860408 22 22.07496092256028 26.080348893393445 30.090315357456703 21.75437482658957 23 21.80013692387661 26.02200732356553 30.01642415024633 22.16143160231153 24 21.40451158206344 26.22240699719205 30.2442234674183 22.128857953326207 25 21.696828069777034 26.125516489037476 30.295094296544754 21.88256114464074 26 21.59248582177242 26.22186754816873 30.39258815351349 21.79305847654536 27 21.59612058899399 26.49871556328236 30.055727610886652 21.849436236837004 28 21.583383503913307 26.13411969865901 30.23121411989405 22.051282677533635 29 21.622793464759557 26.298364235717138 30.3026768495324 21.7761654499909 30 21.737554875777725 26.375084717820968 30.135853802990614 21.751506603410693 31 21.96376205751466 26.069261666955125 30.05913489612093 21.907841379409284 32 21.394339209159426 26.50412256032758 30.00541454210493 22.096123688408056 33 21.63949297203618 26.144922776487522 30.019177301985888 22.19640694949041 34 21.756760386436863 26.17932101290117 30.247914410559257 21.816004190102714 35 21.99771528885604 26.40724442044911 29.942625855202543 21.65241443549231 36 21.94756590886755 26.249703181950533 29.746784445696488 22.05594646348543 37 21.872565991390783 26.01212029465696 30.052421983538473 22.062891730413785 38 21.85057368834178 26.15991982157615 29.983562690664854 22.005943799417217 39 21.839274459743837 26.60042637545762 29.69651650945916 21.863782655339385 40 22.197272531102247 26.145940127049105 29.900002167031754 21.75678517481689 41 21.87924915982045 26.391542136557295 29.645648875604593 22.083559828017666 42 22.000720276390805 26.467990428869655 29.331272203435354 22.20001709130419 43 21.677856814534515 26.196422042037682 29.52416589701971 22.601555246408093 44 21.835213068342227 26.533060150401184 29.261115046619242 22.370611734637354 45 22.025001060556722 26.360312777251654 29.267764172471246 22.346921989720382 46 22.26218429538337 26.279588295361396 28.882589224950088 22.57563818430515 47 22.14377665506311 26.768992810190618 28.623486303715275 22.463744231030997 48 22.08790444118493 26.819455050086095 28.395834897474725 22.696805611254252 49 22.31835140269629 26.664215031777154 28.32672866963203 22.690704895894527 50 22.258070422793107 26.72822099744261 28.355763890160464 22.657944689603816 51 22.12834110685652 27.088482663003653 27.95065554864146 22.832520681498362 52 22.35505629773497 27.28772302259051 27.671159213913082 22.68606146576144 53 22.380429365717088 26.99482755516303 28.05284566863821 22.57189741048167 54 22.367649275845295 26.907341523065586 28.01250981907271 22.7124993820164 55 22.045422154920193 26.82988832603804 28.17987585949705 22.944813659544717 56 22.33217691677799 27.203440499484056 27.778199976133077 22.68618260760488 57 22.33608733707614 26.913031589253038 27.738256115023603 23.01262495864722 58 22.28103686157082 26.653417859583193 28.146350082840648 22.919195196005337 59 22.19298398214453 27.01164737949984 27.96201226235918 22.83335637599644 60 22.067614091187497 27.191085782437014 28.143861145045552 22.597438981329937 61 22.434203298380105 26.893028479351557 28.05139833249918 22.621369889769163 62 22.27189059662083 27.066743997924263 28.1544895373861 22.506875868068803 63 22.079772731920173 26.878515774383693 28.173715535835225 22.867995957860916 64 22.225996729303134 26.82288852954768 28.20927210207895 22.74184263907024 65 22.047693771398695 26.88806794959882 28.134026292101836 22.93021198690065 66 22.183169096540954 27.089730550729453 27.906295289246813 22.82080506348278 67 22.259922012712995 27.001030164750052 27.92247056399612 22.81657725854083 68 22.37219028630609 26.840963207443792 27.98757332856355 22.799273177686565 69 22.312008286754924 27.075244296294215 27.742408640297096 22.870338776653764 70 22.25309389961682 26.63189355263857 28.08735256934224 23.027659978402372 71 22.189518147302625 26.763975193192778 27.961343132347732 23.085163527156862 72 22.196060356786933 27.09962959967157 27.77178333946343 22.932526704078064 73 22.173271727940918 26.794866434329002 27.866999251957775 23.1648625857723 74 22.1735300366041 27.080469186871365 27.510610060144025 23.235390716380515 75 22.030133700365916 27.15362571832284 27.76029761377959 23.055942967531657 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2607.0 1 1730.5 2 1142.0 3 1431.0 4 1432.0 5 1106.5 6 967.0 7 1954.5 8 2756.0 9 4689.5 10 7615.0 11 8122.0 12 7637.0 13 6758.5 14 5955.5 15 6604.5 16 7178.0 17 7860.5 18 9338.0 19 11226.5 20 12320.0 21 13951.0 22 17694.5 23 22349.0 24 24891.0 25 27602.5 26 34176.0 27 41864.5 28 45691.0 29 51472.0 30 61433.5 31 70499.0 32 75384.0 33 80253.5 34 90677.5 35 101759.5 36 107287.0 37 110540.0 38 116361.5 39 121641.0 40 124352.0 41 128843.5 42 134862.0 43 139769.0 44 143149.0 45 147069.5 46 151408.0 47 152655.5 48 153485.0 49 152980.5 50 148559.0 51 142017.5 52 139393.0 53 135736.0 54 128135.5 55 117787.5 56 111383.0 57 108860.0 58 100313.0 59 86176.5 60 78064.0 61 70408.0 62 56261.0 63 41802.5 64 33835.0 65 28653.0 66 19365.5 67 13680.5 68 12101.0 69 9928.0 70 6148.0 71 3917.0 72 3293.0 73 3310.0 74 3212.0 75 2229.5 76 1362.0 77 1136.5 78 650.0 79 321.5 80 254.0 81 236.0 82 149.5 83 56.5 84 32.0 85 24.5 86 12.5 87 10.5 88 13.0 89 8.0 90 3.5 91 4.0 92 4.0 93 2.5 94 1.5 95 1.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07556180015307674 2 0.016754211746380906 3 0.002258309051424749 4 0.13702442757969083 5 0.09820592604709245 6 0.07025172211324016 7 0.03045665450434999 8 0.027343850136169932 9 0.0510560951761298 10 0.03137218520087354 11 0.017639224753020336 12 0.11010782510189857 13 0.09075960971536762 14 0.05114764824578215 15 0.020935135260505104 16 0.03390515346125535 17 0.023681727350075746 18 0.019867016114560966 19 0.015960751809393832 20 0.01449590269495616 21 0.029571641497710563 22 0.018341131620355056 23 0.015716610290320888 24 0.022308431305290425 25 0.01773077782267269 26 0.03561414409476597 27 0.03152477365029413 28 0.03955092608981722 29 0.05322285115790219 30 0.03686536938001482 31 0.021942219026681004 32 0.013000535890634365 33 0.01583868104985736 34 0.01513677418252264 35 0.0068664802239266015 36 0.011291545257123744 37 0.009155306965235468 38 0.0029296982288753498 39 0.010162390731411371 40 0.012725876681677302 41 0.012359664403067882 42 0.008178740888943686 43 0.012023969814342582 44 0.010711709149325498 45 0.006317161806012474 46 0.002136238291888276 47 0.006286644116128354 48 0.007019068673347192 49 0.0019226144626994484 50 2.4414151907294583E-4 51 0.0020446852222359216 52 0.003112804368180059 53 3.051768988411823E-5 54 6.103537976823646E-5 55 5.188007280300099E-4 56 0.008758576996741931 57 0.0031738397479482957 58 4.5776534826177343E-4 59 0.007843046300218384 60 0.0013732960447853204 61 8.544953167553104E-4 62 0.025573824122891074 63 0.00930789541465606 64 0.012878465131097892 65 0.018158025481050347 66 0.019043038487689776 67 0.018707343898964474 68 0.018585273139428003 69 0.007141139432883666 70 0.01531988032182735 71 0.008178740888943686 72 0.01840216700012329 73 0.0071716571227677835 74 0.019897533804445085 75 0.0017700260132788575 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3276788.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.894289002469264 #Duplication Level Percentage of deduplicated Percentage of total 1 72.2709838220205 36.781803371306914 2 12.817237498471737 13.046483789210136 3 5.584375883193692 8.526385202930378 4 2.9307612684685007 5.96636043978717 5 1.7802875400248337 4.530323428475948 6 1.101545196058912 3.363741573450235 7 0.7617647287910682 2.7138631981286165 8 0.5258419743258012 2.1409882728773075 9 0.4051434774664372 1.8557540306678024 >10 1.709922255482203 14.436001844107116 >50 0.07537909908623062 2.6329950773512514 >100 0.035191628102803726 3.1098630349361573 >500 0.0010838966606384 0.33083166541509346 >1k 4.215153659185997E-4 0.4015950092302411 >5k 6.0216480845514245E-5 0.1630100621257366 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5319 0.16232359249362485 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3680 0.11230509877355507 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 3.051768988411823E-5 0.0 12 0.0 0.0 0.0 3.051768988411823E-5 0.0 13 0.0 0.0 0.0 3.051768988411823E-5 0.0 14 0.0 0.0 0.0 3.051768988411823E-5 0.0 15 0.0 0.0 0.0 3.051768988411823E-5 0.0 16 0.0 0.0 0.0 3.051768988411823E-5 0.0 17 0.0 0.0 0.0 9.155306965235468E-5 0.0 18 0.0 0.0 0.0 9.155306965235468E-5 0.0 19 0.0 0.0 0.0 1.2207075953647292E-4 0.0 20 0.0 0.0 0.0 1.2207075953647292E-4 0.0 21 0.0 0.0 0.0 1.5258844942059115E-4 0.0 22 0.0 0.0 0.0 2.136238291888276E-4 0.0 23 0.0 0.0 0.0 4.5776534826177343E-4 0.0 24 0.0 0.0 0.0 7.324245572188374E-4 0.0 25 0.0 0.0 0.0 8.544953167553104E-4 0.0 26 0.0 0.0 0.0 0.001068119145944138 0.0 27 0.0 0.0 0.0 0.0014038137346694384 0.0 28 0.0 0.0 0.0 0.002288826741308867 0.0 29 0.0 0.0 0.0 0.003875746615283015 0.0 30 0.0 0.0 0.0 0.006134055666707764 0.0 31 0.0 0.0 0.0 0.012085005194110819 0.0 32 0.0 0.0 0.0 0.018585273139428 0.0 33 0.0 0.0 0.0 0.025360200293702246 0.0 34 0.0 0.0 0.0 0.03533948488580891 0.0 35 0.0 0.0 0.0 0.04672258321258501 0.0 36 0.0 0.0 0.0 0.062439193502905894 0.0 37 0.0 0.0 0.0 0.08251983344665569 0.0 38 0.0 0.0 0.0 0.10537758316986025 0.0 39 0.0 0.0 0.0 0.13101244267251955 0.0 40 0.0 0.0 0.0 0.15701351445378828 0.0 41 0.0 0.0 0.0 0.18338079851366643 0.0 42 0.0 0.0 0.0 0.2094429056747034 0.0 43 0.0 0.0 0.0 0.23541345976608802 0.0 44 0.0 0.0 0.0 0.2671823749354551 0.0 45 0.0 0.0 0.0 0.30410877969523814 0.0 46 0.0 0.0 0.0 0.34213382129084946 0.0 47 0.0 0.0 0.0 0.38366839722313434 0.0 48 0.0 0.0 0.0 0.4248367608768098 0.0 49 0.0 0.0 0.0 0.46832446896167834 0.0 50 0.0 0.0 0.0 0.5129413315722592 0.0 51 0.0 0.0 0.0 0.5597859855443806 0.0 52 0.0 0.0 0.0 0.6054404496110215 0.0 53 0.0 0.0 0.0 0.6521630328236065 0.0 54 0.0 0.0 0.0 0.7034327518289252 0.0 55 0.0 0.0 0.0 0.7589749474180203 0.0 56 0.0 0.0 0.0 0.8179046065842527 0.0 57 0.0 0.0 0.0 0.8831514275564974 0.0 58 0.0 0.0 0.0 0.9508091460295875 0.0 59 0.0 0.0 0.0 1.0236243540930936 0.0 60 0.0 0.0 0.0 1.1001932380123463 0.0 61 0.0 0.0 0.0 1.1799664793694313 0.0 62 3.051768988411823E-5 0.0 0.0 1.2573898586054393 0.0 63 3.051768988411823E-5 0.0 0.0 1.3313647388845418 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6585 0.0 35.978985 1 GTATCAA 13205 0.0 34.835926 1 ATCAACG 18535 0.0 24.755184 3 TATCAAC 18835 0.0 24.376633 2 TCAACGC 18920 0.0 24.23391 4 CAACGCA 19160 0.0 23.93129 5 AACGCAG 19775 0.0 23.186321 6 GTACATG 13730 0.0 21.538212 1 TACATGG 14015 0.0 20.475113 2 ACGCAGA 22500 0.0 20.272644 7 GAGTACT 15555 0.0 20.268305 12 AGAGTAC 21775 0.0 20.013262 11 AGTACTT 15975 0.0 19.921144 13 CGCAGAG 22800 0.0 19.915335 8 GTACTTT 16285 0.0 19.213434 14 ACATGGG 14430 0.0 19.193493 3 GCAGAGT 23850 0.0 18.705566 9 CAGAGTA 23835 0.0 18.602295 10 TACACCG 205 3.794412E-8 18.526884 5 TACTTTT 17760 0.0 17.654684 15 >>END_MODULE