FastQCFastQC Report
Mon 6 Feb 2017
SRR2077265_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077265_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4484364
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40100.036.1635931
GTATCAA82900.034.0278241
TCAACGC107700.025.5019074
CAACGCA110050.025.177665
TATCAAC110800.025.0669982
ATCAACG109750.025.0564423
AACGCAG114100.024.4133536
ACGCAGA133450.020.6165077
GTACATG135250.020.244291
CGCAGAG137100.019.9942048
TACATGG138600.019.5163672
GAGTACT71700.019.43098612
AGAGTAC133400.019.12907611
ACATGGG142850.018.3568823
CGAATCG3103.6379788E-1217.80915537
CAGAGTA146050.017.77837810
GTACTTT77900.017.75287414
AGTACTT78600.017.59457413
GCAGAGT151800.017.3321139
GTACCGA5950.016.2234346
Produced by FastQC (version 0.11.5)