##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077265_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4484364 Sequences flagged as poor quality 0 Sequence length 75 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.127929846908057 31.0 31.0 34.0 26.0 34.0 2 30.218204632808575 31.0 31.0 34.0 26.0 34.0 3 30.26877278472488 31.0 31.0 34.0 26.0 34.0 4 33.689441802672576 37.0 35.0 37.0 28.0 37.0 5 33.722576267225406 37.0 35.0 37.0 28.0 37.0 6 33.727077908929786 37.0 35.0 37.0 28.0 37.0 7 33.64684958669724 37.0 35.0 37.0 28.0 37.0 8 33.57853644351797 37.0 35.0 37.0 28.0 37.0 9 35.02845308721594 39.0 35.0 39.0 27.0 39.0 10 34.94034761674119 39.0 35.0 39.0 27.0 39.0 11 34.992596720516 39.0 35.0 39.0 27.0 39.0 12 34.90820638110555 39.0 35.0 39.0 27.0 39.0 13 34.88317540681354 39.0 35.0 39.0 27.0 39.0 14 36.02892918594476 40.0 36.0 41.0 27.0 41.0 15 36.05312124528696 40.0 36.0 41.0 27.0 41.0 16 36.030218777958254 40.0 36.0 41.0 27.0 41.0 17 35.95050156499339 40.0 36.0 41.0 27.0 41.0 18 35.9120675752459 40.0 36.0 41.0 27.0 41.0 19 35.99546914568041 40.0 36.0 41.0 27.0 41.0 20 35.98045118549698 40.0 36.0 41.0 27.0 41.0 21 35.953789879679704 40.0 36.0 41.0 27.0 41.0 22 35.883758321135396 40.0 36.0 41.0 27.0 41.0 23 35.83569130427414 40.0 36.0 41.0 27.0 41.0 24 35.807760476179006 40.0 36.0 41.0 26.0 41.0 25 35.83357996808466 40.0 36.0 41.0 27.0 41.0 26 35.77551376293272 40.0 36.0 41.0 26.0 41.0 27 35.76940921834178 40.0 36.0 41.0 26.0 41.0 28 35.72822433682904 40.0 36.0 41.0 26.0 41.0 29 35.695377761484124 40.0 36.0 41.0 26.0 41.0 30 35.60786635518437 40.0 36.0 41.0 25.0 41.0 31 35.59069625034899 40.0 36.0 41.0 25.0 41.0 32 35.522234591125965 40.0 35.0 41.0 25.0 41.0 33 35.41552425271454 40.0 35.0 41.0 25.0 41.0 34 35.373917906753334 40.0 35.0 41.0 25.0 41.0 35 35.32468394626306 40.0 35.0 41.0 25.0 41.0 36 35.24951431239748 40.0 35.0 41.0 24.0 41.0 37 35.17820052074274 40.0 35.0 41.0 24.0 41.0 38 35.093617734867195 40.0 35.0 41.0 24.0 41.0 39 35.05073473072213 40.0 35.0 41.0 24.0 41.0 40 35.00468048534864 40.0 35.0 41.0 24.0 41.0 41 34.92393235696299 40.0 35.0 41.0 23.0 41.0 42 34.83416578136833 40.0 35.0 41.0 23.0 41.0 43 34.730416174958144 39.0 35.0 41.0 22.0 41.0 44 34.63610737219369 39.0 35.0 41.0 22.0 41.0 45 34.53945643127989 39.0 34.0 41.0 21.0 41.0 46 34.43748812540641 39.0 34.0 41.0 20.0 41.0 47 34.331630304765625 39.0 34.0 41.0 19.0 41.0 48 34.22164659247108 39.0 34.0 41.0 18.0 41.0 49 34.1215278242355 39.0 34.0 41.0 16.0 41.0 50 33.99592272170591 39.0 34.0 41.0 15.0 41.0 51 33.89164639623367 39.0 33.0 41.0 14.0 41.0 52 33.786601622883424 39.0 33.0 40.0 13.0 41.0 53 33.69698088736775 38.0 33.0 40.0 13.0 41.0 54 33.69149047668744 38.0 33.0 40.0 12.0 41.0 55 33.588896887050204 38.0 33.0 40.0 9.0 41.0 56 33.452704329978566 38.0 33.0 40.0 8.0 41.0 57 33.352273365855226 38.0 33.0 40.0 8.0 41.0 58 33.250847388838196 38.0 33.0 40.0 7.0 41.0 59 33.13094833514853 38.0 33.0 40.0 6.0 41.0 60 33.09766892250495 38.0 33.0 40.0 2.0 41.0 61 32.983776963689834 37.0 33.0 40.0 2.0 41.0 62 32.82432915793633 37.0 33.0 40.0 2.0 41.0 63 32.63084954744976 37.0 32.0 40.0 2.0 41.0 64 32.47998311466242 37.0 32.0 40.0 2.0 41.0 65 32.32703522729199 36.0 32.0 40.0 2.0 41.0 66 32.154164336347364 36.0 32.0 39.0 2.0 41.0 67 31.984142901869696 36.0 32.0 39.0 2.0 41.0 68 31.7913160037856 36.0 32.0 39.0 2.0 41.0 69 31.82506326426668 36.0 32.0 39.0 2.0 41.0 70 31.72562040012809 35.0 32.0 39.0 2.0 41.0 71 31.56314630123692 35.0 32.0 39.0 2.0 41.0 72 31.362487746311405 35.0 32.0 39.0 2.0 40.0 73 31.15511920976977 35.0 32.0 38.0 2.0 40.0 74 30.94021270351827 35.0 32.0 37.0 2.0 40.0 75 30.72490234958625 35.0 32.0 37.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7222808059812458 1101 2 0.7444518667594799 1101 3 0.7367367200641723 1101 4 0.7457208243318334 1101 5 0.7616711687065276 1101 6 0.7032512435523444 1101 7 0.7406962465961158 1101 8 0.7443057964615036 1101 9 0.8540977445325879 1101 10 0.9371761595044461 1101 11 0.8500411985990795 1101 12 0.8646621867488165 1101 13 0.9240942168103743 1101 14 1.0457398077137654 1101 15 1.0813233079207265 1101 16 1.086592580138138 1101 17 1.0351854188700216 1101 18 1.0874016817814507 1101 19 1.1054321365322437 1101 20 1.148097558848498 1101 21 1.0809648884729626 1101 22 1.1285718298658338 1101 23 1.1565997462001434 1101 24 1.1125757571513617 1101 25 1.0140767373644408 1101 26 1.048898083253377 1101 27 1.1175644329778152 1101 28 1.091526239547882 1101 29 1.1560406823133107 1101 30 1.1483363593303295 1101 31 1.1367545337675438 1101 32 1.1823527919089756 1101 33 1.2209985954387221 1101 34 1.1592778635149656 1101 35 1.1228883466809876 1101 36 1.1683430007668107 1101 37 1.1591121784698188 1101 38 1.1769677504079823 1101 39 1.1776717797780307 1101 40 1.1503336416101249 1101 41 1.1901926515135273 1101 42 1.073364597892386 1101 43 0.9932711283298872 1101 44 1.0743509114115355 1101 45 1.0957017786686194 1101 46 1.1335228290994053 1101 47 1.0265636229138906 1101 48 1.0035681817642583 1101 49 1.0058210682849165 1101 50 1.0215350003236807 1101 51 0.9943338626984399 1101 52 1.0526078450446477 1101 53 1.1107839579572385 1101 54 1.1080843627511356 1101 55 1.070578461706262 1101 56 1.0254819852493284 1101 57 1.1085228904426145 1101 58 1.1138480611644752 1101 59 1.0545942799057002 1101 60 1.1620367895871624 1101 61 1.0146463537999537 1101 62 1.0064300570892186 1101 63 1.0234433089513502 1101 64 1.0089539177429288 1101 65 0.9490706277718459 1101 66 0.9049461332151623 1101 67 0.8853355185069276 1101 68 0.9066368901356334 1101 69 0.9053305621654175 1101 70 0.9535100639524146 1101 71 0.9442626833888283 1101 72 0.88360325112982 1101 73 0.846739589094323 1101 74 0.8717024174464605 1101 75 0.8052297600878155 1102 1 0.7738761913432022 1102 2 0.7376345868263066 1102 3 0.7942328134527976 1102 4 0.8350198523485304 1102 5 0.7840188651336888 1102 6 0.6933498549308297 1102 7 0.7910521996075346 1102 8 0.7677425752861211 1102 9 0.9699667800753247 1102 10 0.9625487191003401 1102 11 0.9716359191448944 1102 12 0.9219661216412618 1102 13 0.8893344583974283 1102 14 1.1383623385286015 1102 15 1.1146512242336968 1102 16 1.0157616971522572 1102 17 1.0536846941802338 1102 18 1.070346987111705 1102 19 1.0686814723819253 1102 20 1.0675212903926266 1102 21 1.1306999695440965 1102 22 1.1749377302954755 1102 23 1.1647980396980273 1102 24 1.2382246501118956 1102 25 1.1576102647758475 1102 26 1.1859685224576495 1102 27 1.191287341531826 1102 28 1.152276540084472 1102 29 1.160911452960839 1102 30 1.2132272634043986 1102 31 1.191598875084253 1102 32 1.1982363197399764 1102 33 1.2603980468724458 1102 34 1.270236631397104 1102 35 1.234188090367482 1102 36 1.164583925310481 1102 37 1.195574177745307 1102 38 1.233204034806377 1102 39 1.1844885069540183 1102 40 1.2023231177164178 1102 41 1.1303171897163082 1102 42 1.1610881423181638 1102 43 1.1172587598352166 1102 44 1.0373792700156699 1102 45 1.0551170399627168 1102 46 1.1289219798332226 1102 47 1.0501736408639246 1102 48 1.0807412247613826 1102 49 1.1205498662656836 1102 50 1.1244046442510722 1102 51 1.1376547544726492 1102 52 1.1441502883288663 1102 53 1.1375814501883639 1102 54 1.0823737670140687 1102 55 1.0863460110306704 1102 56 1.1024842262776886 1102 57 1.0208175116278113 1102 58 1.020950282399106 1102 59 0.9887659499042343 1102 60 1.0869404804423297 1102 61 1.0570438345538307 1102 62 1.0821390724432547 1102 63 1.1081728692345223 1102 64 1.0418040005987166 1102 65 1.0800814915107253 1102 66 1.0927590785772736 1102 67 1.1417608810364968 1102 68 1.0989399323252265 1102 69 1.0839360050447766 1102 70 1.0819087228830924 1102 71 1.1160711224734072 1102 72 1.1220117697410785 1102 73 1.080934188465715 1102 74 1.1240279045926158 1102 75 1.137941141091833 1103 1 0.5591507610678583 1103 2 0.5163898795438584 1103 3 0.5744715931977673 1103 4 0.6025656780756208 1103 5 0.6058289135396961 1103 6 0.5416266724906791 1103 7 0.5675193754058867 1103 8 0.5913159778262056 1103 9 0.6165882004434664 1103 10 0.6517740938328274 1103 11 0.6252241646881487 1103 12 0.6240922696585258 1103 13 0.6303207043404271 1103 14 0.663005618000831 1103 15 0.6955885044363512 1103 16 0.6792665671747997 1103 17 0.7321452796583898 1103 18 0.7702494115645635 1103 19 0.8104264691474299 1103 20 0.7960401025811308 1103 21 0.7264869984260969 1103 22 0.7680476030597561 1103 23 0.7632708838338615 1103 24 0.8712102752761623 1103 25 0.7423139821477704 1103 26 0.8778794254007849 1103 27 0.8075412193656746 1103 28 0.8842144671867942 1103 29 0.8516292130595176 1103 30 0.808430398604898 1103 31 0.7972152673169006 1103 32 0.8265005628946369 1103 33 0.8453032685611603 1103 34 0.7926383906273813 1103 35 0.8922045989865737 1103 36 0.8490325665389307 1103 37 0.8853718687507808 1103 38 0.8829323627826824 1103 39 0.8800587799579205 1103 40 0.8602194509215053 1103 41 0.8927217370927423 1103 42 0.8939551325335273 1103 43 0.8324801528959895 1103 44 0.8783274923045923 1103 45 0.8399892229806909 1103 46 0.887604103328961 1103 47 0.8573623312858416 1103 48 0.794582657703927 1103 49 0.7736228692894969 1103 50 0.7878207222479787 1103 51 0.7850995103802134 1103 52 0.8113994931953243 1103 53 0.8473761004496865 1103 54 0.8441836087489776 1103 55 0.7793926003404721 1103 56 0.8153496167056957 1103 57 0.8366830870073159 1103 58 0.8174550819047042 1103 59 0.7971291732040271 1103 60 0.7753119016955239 1103 61 0.8424735511206904 1103 62 0.7890774516385903 1103 63 0.8309199605541551 1103 64 0.8321711948539487 1103 65 0.9040986628168497 1103 66 0.7917715734714932 1103 67 0.8837522565369227 1103 68 0.8677439161784726 1103 69 0.8363548462150732 1103 70 0.7695828205556623 1103 71 0.7803557931521041 1103 72 0.8664178482058702 1103 73 0.8731659195782022 1103 74 0.9440779477017216 1103 75 0.9675714419063972 1104 1 0.6559798949493825 1104 2 0.676694259798559 1104 3 0.6631160422781832 1104 4 0.7378147189833513 1104 5 0.6592685839590331 1104 6 0.5966053213798261 1104 7 0.5813290578992749 1104 8 0.5991805344283421 1104 9 0.7266098838673258 1104 10 0.679510368382445 1104 11 0.6654413312397338 1104 12 0.7125529773375376 1104 13 0.6912047400234584 1104 14 0.8311564622604948 1104 15 0.7616216378895686 1104 16 0.7838059628820488 1104 17 0.8156931087313524 1104 18 0.8637955236869743 1104 19 0.8143251930654714 1104 20 0.8237504844087766 1104 21 0.8335925890386235 1104 22 0.8501091621985495 1104 23 0.8784630807551679 1104 24 0.9169121757373446 1104 25 0.8541410021493192 1104 26 0.8906448142623944 1104 27 0.9104984820664939 1104 28 0.9251714917299552 1104 29 0.8727890131547866 1104 30 0.8892854769015344 1104 31 0.9299486525895233 1104 32 0.8370770244257173 1104 33 0.9412306341682424 1104 34 0.9066414608303504 1104 35 0.9327228318936847 1104 36 0.9099736954431492 1104 37 0.8646944302194584 1104 38 0.8988211708497715 1104 39 0.9398301670693456 1104 40 0.8697239260700371 1104 41 0.8879499530518871 1104 42 0.8160347473458245 1104 43 0.8959625070751969 1104 44 0.9722519797117783 1104 45 0.8506287778289519 1104 46 0.8915968441358899 1104 47 0.9142009176263031 1104 48 0.9630125987368316 1104 49 0.9763949136422028 1104 50 0.9445980153000519 1104 51 0.9857101355348306 1104 52 0.9942878194085409 1104 53 0.9501768991086124 1104 54 0.9355761017980981 1104 55 0.8972124239191857 1104 56 0.9900084881707159 1104 57 0.8469335587071711 1104 58 0.8369338858732576 1104 59 0.8524614123660115 1104 60 0.9128829797558495 1104 61 0.9811692779446233 1104 62 0.9601339116188754 1104 63 0.9334985710426906 1104 64 0.9265142120345331 1104 65 0.9933337982713297 1104 66 0.9896677090404324 1104 67 0.975150888145798 1104 68 1.0625271096326507 1104 69 1.0121673976774304 1104 70 0.9520569555264373 1104 71 0.9432906154938792 1104 72 0.9749241843072411 1104 73 0.9726666192817071 1104 74 0.9460325482060981 1104 75 0.93009871926591 1105 1 0.40850869883576024 1105 2 0.4424743292288227 1105 3 0.45454137787570303 1105 4 0.44558742443494737 1105 5 0.3524131067424108 1105 6 0.41293733095245955 1105 7 0.4923010009793529 1105 8 0.4552795065881696 1105 9 0.5291803351640425 1105 10 0.6043406887719485 1105 11 0.6244030478653784 1105 12 0.599466465366369 1105 13 0.5284666780521121 1105 14 0.601122589033416 1105 15 0.5821689923385875 1105 16 0.6784270878816017 1105 17 0.6366498169200341 1105 18 0.6221115711059113 1105 19 0.6380155387479363 1105 20 0.6718681110258586 1105 21 0.6617659425776665 1105 22 0.7889272355624755 1105 23 0.6716406527738386 1105 24 0.753335836482016 1105 25 0.6821524233918268 1105 26 0.6972324941523382 1105 27 0.7410138978778207 1105 28 0.7375186502122304 1105 29 0.7033335373711864 1105 30 0.8012620182139756 1105 31 0.6951681389202164 1105 32 0.6924587207023478 1105 33 0.8351196156069278 1105 34 0.6995433538618911 1105 35 0.7899778555565788 1105 36 0.759127223023583 1105 37 0.7030059277707679 1105 38 0.7474282664937206 1105 39 0.7522024931264752 1105 40 0.7191955413446891 1105 41 0.7106827665538873 1105 42 0.7113666548151301 1105 43 0.6993917011037638 1105 44 0.7954932946593445 1105 45 0.8273742969258535 1105 46 0.7487691551462134 1105 47 0.7262419900336781 1105 48 0.6738740194419535 1105 49 0.6683561573682226 1105 50 0.7320776841986643 1105 51 0.7503416320830212 1105 52 0.7298251068276187 1105 53 0.7663830708070094 1105 54 0.7173508815594261 1105 55 0.7004592202033422 1105 56 0.756378383452109 1105 57 0.7307843051362397 1105 58 0.6808235390443755 1105 59 0.6807090147290751 1105 60 0.6923272958247679 1105 61 0.7234485645827036 1105 62 0.7584617457027605 1105 63 0.8327332886822063 1105 64 0.7478447617086914 1105 65 0.8072739030257594 1105 66 0.7777647448195921 1105 67 0.8070837937199187 1105 68 0.8527667927182456 1105 69 0.8326862706716369 1105 70 0.8579068045209866 1105 71 0.883142888099016 1105 72 0.8801618611520041 1105 73 0.8712917173546408 1105 74 0.9400241703592975 1105 75 0.8557198478480359 1106 1 0.5115865485783253 1106 2 0.5339029429941142 1106 3 0.57640787345251 1106 4 0.5702120117827008 1106 5 0.5129329646348779 1106 6 0.5142099157737192 1106 7 0.4992492085587372 1106 8 0.5746813428492601 1106 9 0.6909295331478233 1106 10 0.7348037479330003 1106 11 0.661154374290561 1106 12 0.5111829189311052 1106 13 0.6238711553569516 1106 14 0.6878410323929813 1106 15 0.7367551238953709 1106 16 0.687916708648153 1106 17 0.6590074852816983 1106 18 0.7066107595685764 1106 19 0.721406139611382 1106 20 0.7497404269854329 1106 21 0.7499973015795902 1106 22 0.8386224752496716 1106 23 0.7868773512715919 1106 24 0.8635135601471973 1106 25 0.7520561363858107 1106 26 0.8764132047411053 1106 27 0.822574039139603 1106 28 0.7853263469076239 1106 29 0.8689159879050123 1106 30 0.7967938727170534 1106 31 0.7743911757054462 1106 32 0.7990813948141593 1106 33 0.845097163962194 1106 34 0.8070883525791928 1106 35 0.733179774043748 1106 36 0.7059231204197545 1106 37 0.706998017291042 1106 38 0.7482667749183847 1106 39 0.7337226391871638 1106 40 0.75545729375321 1106 41 0.7119561702564212 1106 42 0.7407973559911056 1106 43 0.7668743417956847 1106 44 0.7238657932641104 1106 45 0.7585723691840229 1106 46 0.8298349780792122 1106 47 0.8182427808390216 1106 48 0.6966002277023549 1106 49 0.6977187050924272 1106 50 0.6272935285200631 1106 51 0.7592993049767003 1106 52 0.8005372159455888 1106 53 0.7819289034833901 1106 54 0.656017410965994 1106 55 0.7441592524689966 1106 56 0.733456965161885 1106 57 0.7177185345423212 1106 58 0.7722550773856298 1106 59 0.7814282047870407 1106 60 0.8130825175251672 1106 61 0.8197987631007564 1106 62 0.8010135894442669 1106 63 0.87638637608773 1106 64 0.8958849340307751 1106 65 0.8982877902555799 1106 66 0.8712151504452805 1106 67 0.9109127612245871 1106 68 0.8943721570231453 1106 69 0.8407046780827514 1106 70 0.8820275372559507 1106 71 0.8703642458743559 1106 72 0.834210381990296 1106 73 0.8374081001403049 1106 74 0.8899787614431354 1106 75 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2214 16 -0.9788796070733312 2214 17 -0.8695342739790703 2214 18 -1.1478842610128481 2214 19 -0.840880873491507 2214 20 -0.9773827606731302 2214 21 -0.9693915163385753 2214 22 -0.8670235302712541 2214 23 -0.9637850124208569 2214 24 -0.8737842716224549 2214 25 -0.9254307702993785 2214 26 -0.9914320940445833 2214 27 -1.0178744421257448 2214 28 -0.8749860183310219 2214 29 -0.9465831242676686 2214 30 -0.9327303396687086 2214 31 -0.9520098109614921 2214 32 -1.0035033197548415 2214 33 -0.9922494249643776 2214 34 -0.9732306188332132 2214 35 -1.0291583674306963 2214 36 -0.9729150765403531 2214 37 -0.954536939415604 2214 38 -1.0512406467757458 2214 39 -0.9804866794824463 2214 40 -0.952511803580883 2214 41 -0.9395183419992676 2214 42 -1.0154847486219793 2214 43 -0.9893430452715322 2214 44 -0.9952318712140809 2214 45 -1.0409921240215212 2214 46 -1.097188720511923 2214 47 -1.0265993006086518 2214 48 -1.0384883909695972 2214 49 -0.9963152584792923 2214 50 -1.032558771994239 2214 51 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-1.4739544345826943 2215 13 -1.6443442697603032 2215 14 -1.6862040771246924 2215 15 -1.6797203603258666 2215 16 -1.700530916066782 2215 17 -1.9763390182145457 2215 18 -1.829449929817791 2215 19 -1.6423984410257546 2215 20 -1.667515281505331 2215 21 -1.846223590948803 2215 22 -1.7344456129347847 2215 23 -1.8652256726967522 2215 24 -1.7969270154671477 2215 25 -1.761628597138106 2215 26 -1.8184359249380648 2215 27 -1.7807351169135757 2215 28 -1.8428766477236067 2215 29 -1.8079635754895236 2215 30 -1.751733734657705 2215 31 -1.7424638400205055 2215 32 -1.901209448290608 2215 33 -1.7344500855869995 2215 34 -1.8044427628272643 2215 35 -1.6917669468229022 2215 36 -1.7975304719487966 2215 37 -1.9422231267714878 2215 38 -1.7945311529318886 2215 39 -1.8988487579238722 2215 40 -1.8401375228494246 2215 41 -1.8729960762821634 2215 42 -1.85987686474094 2215 43 -1.9111850405538249 2215 44 -1.7086120072787452 2215 45 -1.7710143742404725 2215 46 -1.7475208919392173 2215 47 -1.79555068150745 2215 48 -1.9302237006191234 2215 49 -1.811100323064089 2215 50 -1.8112423512422566 2215 51 -1.774352645852332 2215 52 -1.677926555466989 2215 53 -1.7670162585277858 2215 54 -1.853720855652945 2215 55 -1.890569747327298 2215 56 -1.7509207696649973 2215 57 -1.696309503349724 2215 58 -1.8023862607813008 2215 59 -1.8298430887309216 2215 60 -1.8762061961590533 2215 61 -1.9176620097206794 2215 62 -1.9629281923220745 2215 63 -1.8368997147988395 2215 64 -1.877352288720541 2215 65 -1.8650120607602716 2215 66 -1.8867575742873335 2215 67 -1.806375686720635 2215 68 -1.8743969826756732 2215 69 -1.8985812391972807 2215 70 -1.8994679061224247 2215 71 -1.9338383219662312 2215 72 -1.9322710354628931 2215 73 -1.8610511883016123 2215 74 -1.8446077364781281 2215 75 -1.8520479070676146 2216 1 -1.0488040366955644 2216 2 -0.892987016326348 2216 3 -1.1589856297159145 2216 4 -1.0194956449212498 2216 5 -0.9414936486448511 2216 6 -1.0220632303782224 2216 7 -0.9987837402320281 2216 8 -1.0554527948069676 2216 9 -1.0809740231894693 2216 10 -1.2347123387727024 2216 11 -1.1987675450818784 2216 12 -1.1489204674620694 2216 13 -1.3222495874906741 2216 14 -1.502916564540591 2216 15 -1.4713372384320493 2216 16 -1.4167457337001963 2216 17 -1.3265354995794425 2216 18 -1.5585904487742965 2216 19 -1.5512990385548875 2216 20 -1.4251445856674465 2216 21 -1.5998780281239036 2216 22 -1.5510203558737388 2216 23 -1.5654221540616504 2216 24 -1.512014244228233 2216 25 -1.504286585753313 2216 26 -1.5404146275866353 2216 27 -1.5562521941075929 2216 28 -1.5599311637900826 2216 29 -1.7221104827881746 2216 30 -1.7104993767989853 2216 31 -1.5684561912757289 2216 32 -1.5415051614606838 2216 33 -1.4265097149834673 2216 34 -1.629805859061463 2216 35 -1.6031297621559872 2216 36 -1.5895606664943998 2216 37 -1.6573396243662302 2216 38 -1.677514272966313 2216 39 -1.698243044142849 2216 40 -1.680220750595815 2216 41 -1.64759067886353 2216 42 -1.5649647608912005 2216 43 -1.690146725774575 2216 44 -1.6996314724642758 2216 45 -1.7913170667739493 2216 46 -1.7626907142609625 2216 47 -1.658082091963081 2216 48 -1.744185581740176 2216 49 -1.7105272648677499 2216 50 -1.7566390759219814 2216 51 -1.9054150796114655 2216 52 -1.8070491611668231 2216 53 -1.8999398945968196 2216 54 -1.8487520270437976 2216 55 -1.8671777899977045 2216 56 -1.8298172433267013 2216 57 -1.8165807024189675 2216 58 -1.8027698269371655 2216 59 -1.8130552461046072 2216 60 -1.8543379447585338 2216 61 -1.9156173583966591 2216 62 -1.823513432861084 2216 63 -1.800674345111723 2216 64 -1.845206343657626 2216 65 -1.980842498753507 2216 66 -1.9442270536839317 2216 67 -1.9035112092232715 2216 68 -1.906370419258426 2216 69 -1.7944683289023544 2216 70 -1.8073264966496154 2216 71 -1.7862305303138406 2216 72 -1.7178760984564647 2216 73 -1.6824400318124653 2216 74 -1.72551589044647 2216 75 -1.7917017479089097 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 157934.0 3 24987.0 4 17224.0 5 12931.0 6 10852.0 7 9859.0 8 9615.0 9 9716.0 10 10251.0 11 10951.0 12 12136.0 13 12883.0 14 13208.0 15 13958.0 16 14857.0 17 15746.0 18 16256.0 19 17390.0 20 19268.0 21 22151.0 22 26908.0 23 32495.0 24 34982.0 25 37338.0 26 40806.0 27 44646.0 28 52592.0 29 63472.0 30 71823.0 31 80659.0 32 98350.0 33 124310.0 34 162631.0 35 217914.0 36 308971.0 37 485151.0 38 794222.0 39 1149163.0 40 225758.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.60661643552522 17.671859203860613 13.396991716888499 24.324532643725668 2 15.83828000013397 19.432792130585305 39.42738866231784 25.301539206962882 3 19.271106970953376 24.05355480597067 28.85676793356535 27.818570289510603 4 13.048882949191015 15.858740207352431 36.81966252411451 34.27271431934204 5 14.381559743567696 37.063378889783465 33.978597885695336 14.576463480953503 6 33.12750866261001 35.812889856524095 17.07360857349995 13.985992907365949 7 29.024594677025455 31.786042341051367 20.798950784299453 18.390412197623725 8 26.746379868789077 34.67687245664436 19.413993004167587 19.162754670398968 9 26.446371958026344 16.156887698146907 19.03511944630498 38.36162089752177 10 14.889819980655759 28.866280913055398 33.06720114886583 23.176697957423016 11 35.17906144149552 23.121578842058625 22.844759822206978 18.854599894238877 12 23.71973024221166 24.716951510995116 29.561787633295015 22.001530613498208 13 28.115732338201337 21.84169700142632 25.658761984444954 24.383808675927384 14 22.15903545137444 21.274241976519715 26.73555342476095 29.831169147344895 15 23.779897777588204 28.999618793076998 23.47080082262087 23.749682606713932 16 23.678193724971035 28.24759056432025 25.008910601874305 23.065305108834405 17 22.50469861539056 28.052849570397953 26.307825011661407 23.13462680255008 18 22.552419485389755 27.53259715509648 27.172658023215178 22.742325336298588 19 23.388125373313027 27.09923101425436 27.36100246470363 22.151641147728984 20 23.555467415972235 26.550099090033246 27.36579966795794 22.528633826036582 21 23.799738718936577 26.41335343233484 27.247473907072123 22.539433941656462 22 23.622702661898252 26.147493734811622 27.118879897550407 23.11092370573972 23 23.262336095784843 26.356825340094264 27.094466906426607 23.286371657694282 24 23.05470026904115 26.392476674985517 27.164981679025825 23.38784137694751 25 23.348707802867217 26.28518233557896 27.158055444599633 23.208054416954184 26 23.008407366961166 26.65398231412893 27.219864071333177 23.117746247576733 27 23.152100239975088 26.604305611467332 26.999811004539914 23.24378314401767 28 23.076656719565467 26.642198793390858 27.04951761995163 23.231626867092043 29 23.16356817362344 26.73998975037654 27.05020394248594 23.04623813351408 30 23.238741936507097 26.60218850758758 27.09373823061757 23.065331325287747 31 23.411489585669695 26.307052291921153 26.975613068914463 23.305845053494693 32 23.06458277956349 26.646913370665796 26.940085639807993 23.34841820996272 33 23.1979806338154 26.458786092663157 26.949665077243125 23.39356819627832 34 23.45896681662844 26.485721978244186 26.970038553619247 23.085272651508127 35 23.33518763027553 26.615438921022587 26.953434805823832 23.095938642878046 36 23.451448630055886 26.499914432949616 26.890998301616925 23.157638635377577 37 23.434082606896393 26.19552616737143 27.09028822407954 23.280103001652638 38 23.351317678975967 26.470056589579087 26.96782681224848 23.210798919196463 39 23.424221338893908 26.720582207424798 26.775778437730008 23.079418015951287 40 23.54506829986024 26.35593684259918 27.04227586507384 23.056718992466738 41 23.274547330500013 26.47597671200251 26.94872091625553 23.300755041241956 42 23.357586566023464 26.575742937342252 26.77697271461026 23.289697782024025 43 23.213553104713178 26.38385000344119 26.968472442324316 23.434124449521317 44 23.31655543086382 26.650188888245946 26.76439986621766 23.26885581467258 45 23.35569159362477 26.6036712406377 26.69888597421294 23.341751191524594 46 23.50255944285356 26.36416091706404 26.62721166210259 23.506067977979807 47 23.33291923967041 26.598799992313943 26.575071531701177 23.493209236314467 48 23.209903473850684 26.776775792382505 26.649670065986804 23.363650667780007 49 23.442989117570338 26.551389400186725 26.530691616660064 23.474929865582872 50 23.477356602693654 26.65813524332324 26.515480488168464 23.349027665814642 51 23.33687760246175 26.760252657140132 26.381030016614183 23.521839723783938 52 23.526368437613975 26.761948351939125 26.295246583375647 23.41643662707125 53 23.409279939646737 26.709446688771767 26.509848298966745 23.371425072614752 54 23.390947362682393 26.594596623593553 26.646499745511598 23.367956268212456 55 23.276466892309177 26.57695783011389 26.517970218406102 23.62860505917083 56 23.226672983748415 26.886900398691292 26.5058641589044 23.38056245865589 57 23.42694811578763 26.611081120696127 26.28186680204062 23.680103961475623 58 23.288447342230967 26.487130152090582 26.606208288848514 23.618214216829934 59 23.35528249553011 26.68742147375969 26.423664950535592 23.533631080174608 60 23.225485151409618 26.82270699487992 26.64842398717141 23.303383866539047 61 23.571390736883792 26.437113255522732 26.633319692676217 23.35817631491725 62 23.22247327037558 26.735771470875928 26.580150426225522 23.461604832522966 63 23.187713714982603 26.53849636314249 26.654437614507636 23.61935230736728 64 23.329530453068777 26.49513769272757 26.58088131750131 23.59445053670234 65 23.23835556899016 26.54895391951521 26.610281584082617 23.602408927412014 66 23.186649886496944 26.620192659055693 26.534034488746805 23.659122965700554 67 23.268073383297832 26.512017700907332 26.488344659806774 23.73156425598806 68 23.269756570922336 26.608061797489142 26.63269032465405 23.489491306934475 69 23.270238422201874 26.606526827050995 26.413445269982322 23.709789480764808 70 23.13027172722681 26.352042982875684 26.661965787132853 23.855719502764654 71 23.176342807593116 26.500140795337217 26.550670677471405 23.772845719598266 72 23.074791414226517 26.661434236985258 26.435048220445008 23.828726128343217 73 23.19122766479414 26.47224979586072 26.551559078823463 23.784963460521677 74 23.173619014567787 26.58333130354316 26.459644456739884 23.783405225149167 75 23.241434020009507 26.668921288585718 26.46267479373674 23.626969897668033 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2464.0 1 2264.5 2 2346.5 3 2483.0 4 2338.0 5 2028.0 6 1644.0 7 1600.5 8 1631.0 9 2157.0 10 3200.5 11 3770.5 12 3823.0 13 3736.5 14 3848.0 15 4273.5 16 4501.0 17 4958.5 18 5964.5 19 7255.5 20 7998.0 21 9311.0 22 12760.5 23 17344.5 24 19792.0 25 23236.5 26 31705.5 27 41136.5 28 45543.0 29 52673.5 30 64816.0 31 77166.0 32 84504.0 33 91120.0 34 104395.0 35 118955.5 36 126857.0 37 132004.0 38 143345.5 39 154156.5 40 158773.0 41 165240.5 42 176517.0 43 187702.0 44 194078.0 45 200387.5 46 211007.0 47 217809.0 48 220301.0 49 223711.0 50 225866.5 51 222337.0 52 220062.0 53 215126.0 54 205718.5 55 193592.5 56 185938.0 57 179472.5 58 162363.0 59 137929.5 60 124140.0 61 112483.0 62 89147.5 63 67420.5 64 57372.0 65 48141.5 66 33126.5 67 23694.0 68 20046.0 69 17345.5 70 12265.5 71 8349.0 72 6812.0 73 6255.5 74 5078.0 75 3493.5 76 2530.0 77 2082.0 78 1359.0 79 884.0 80 684.0 81 566.0 82 347.0 83 191.0 84 136.0 85 115.5 86 85.5 87 62.5 88 49.0 89 42.5 90 27.0 91 23.0 92 28.0 93 23.0 94 16.0 95 18.5 96 23.0 97 17.5 98 12.5 99 14.5 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18707223588450894 2 0.12842400839896137 3 0.12793341486105947 4 0.15096901143618136 5 0.15094671172991309 6 0.12561424540915947 7 0.1246553580396239 8 0.10155286234569717 9 0.1196156244229951 10 0.1229159809506989 11 0.14155853539097185 12 0.14318641394855547 13 0.1583725139172467 14 0.15968819658707453 15 0.1445243963246516 16 0.14610767546969872 17 0.1319473619893479 18 0.1299849878377402 19 0.16854117997557738 20 0.14757945608340448 21 0.16040178718765916 22 0.15264148940630154 23 0.17090494884001386 24 0.1556965491650544 25 0.1808952172481984 26 0.1662889096424822 27 0.17986943075985803 28 0.17879904485898113 29 0.1806722201855157 30 0.17184153650328118 31 0.17911124074673687 32 0.16666800464904277 33 0.18080601842312533 34 0.19576912132913388 35 0.18450776966365798 36 0.18602414968990028 37 0.20283812821617514 38 0.19634891369210883 39 0.1696784649952591 40 0.19799909195596077 41 0.19137607919428484 42 0.24242010684235268 43 0.20444370706749052 44 0.18814262178538585 45 0.18209940138668493 46 0.2132297913371885 47 0.1945872368969156 48 0.22602982273517494 49 0.23314342903475277 50 0.22132458471257016 51 0.23358942316011813 52 0.217912729653525 53 0.209126645383827 54 0.19440883924676944 55 0.1973078010616444 56 0.21684234375264808 57 0.19757539753686362 58 0.22279636532627595 59 0.2350612037738239 60 0.23651068468126135 61 0.222885564151349 62 0.2380716641200402 63 0.2544173488146814 64 0.23552949760545752 65 0.2601929727381631 66 0.22377755240207978 67 0.2435350921557661 68 0.22027649851796152 69 0.21343048869360295 70 0.22353225563312878 71 0.21818032612874422 72 0.21644094903981925 73 0.2113566160106539 74 0.20856915272712026 75 0.23617618908723734 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 4484364.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.170787090240246 #Duplication Level Percentage of deduplicated Percentage of total 1 75.51106805153354 43.170271945308656 2 12.751408499972 14.580161209051578 3 4.814616571491048 8.257662567895716 4 2.33527294663259 5.340375697181191 5 1.283800338310116 3.6697937903953024 6 0.804403868372878 2.759304137598687 7 0.5269021334555485 2.1086386782426363 8 0.37041648194236615 1.694160145907427 9 0.26783272727423774 1.3780987044114443 >10 1.2463402542000437 12.2172746345085 >50 0.0656417509693756 2.542189299294212 >100 0.021513408234747216 1.978733616631572 >500 7.046709103353397E-4 0.2525073685269246 >1k 7.829670095072774E-5 0.05082820504613979 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.2299706268269035E-5 0.0 0.0 0.0 6 0.0 2.2299706268269035E-5 0.0 0.0 2.2299706268269035E-5 7 0.0 2.2299706268269035E-5 0.0 0.0 2.2299706268269035E-5 8 0.0 2.2299706268269035E-5 0.0 0.0 2.2299706268269035E-5 9 0.0 2.2299706268269035E-5 0.0 0.0 2.2299706268269035E-5 10 0.0 2.2299706268269035E-5 0.0 0.0 2.2299706268269035E-5 11 0.0 2.2299706268269035E-5 0.0 0.0 2.2299706268269035E-5 12 0.0 2.2299706268269035E-5 0.0 0.0 2.2299706268269035E-5 13 0.0 2.2299706268269035E-5 0.0 0.0 2.2299706268269035E-5 14 0.0 2.2299706268269035E-5 0.0 0.0 2.2299706268269035E-5 15 0.0 2.2299706268269035E-5 0.0 0.0 4.459941253653807E-5 16 0.0 2.2299706268269035E-5 0.0 0.0 4.459941253653807E-5 17 0.0 2.2299706268269035E-5 0.0 0.0 4.459941253653807E-5 18 0.0 2.2299706268269035E-5 2.2299706268269035E-5 0.0 4.459941253653807E-5 19 0.0 2.2299706268269035E-5 2.2299706268269035E-5 0.0 4.459941253653807E-5 20 0.0 2.2299706268269035E-5 2.2299706268269035E-5 0.0 4.459941253653807E-5 21 0.0 2.2299706268269035E-5 2.2299706268269035E-5 2.2299706268269035E-5 4.459941253653807E-5 22 0.0 2.2299706268269035E-5 2.2299706268269035E-5 4.459941253653807E-5 4.459941253653807E-5 23 0.0 2.2299706268269035E-5 2.2299706268269035E-5 1.7839765014615228E-4 4.459941253653807E-5 24 0.0 2.2299706268269035E-5 2.2299706268269035E-5 3.5679530029230456E-4 4.459941253653807E-5 25 0.0 2.2299706268269035E-5 2.2299706268269035E-5 4.2369441909711163E-4 4.459941253653807E-5 26 0.0 2.2299706268269035E-5 2.2299706268269035E-5 7.135906005846091E-4 4.459941253653807E-5 27 0.0 2.2299706268269035E-5 2.2299706268269035E-5 9.811870758038375E-4 4.459941253653807E-5 28 0.0 2.2299706268269035E-5 2.2299706268269035E-5 0.001984673857875944 4.459941253653807E-5 29 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.003188857996362472 4.459941253653807E-5 30 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.005106632735433609 4.459941253653807E-5 31 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.010057167526989335 4.459941253653807E-5 32 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.016702479994933506 4.459941253653807E-5 33 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.02379378658824306 4.459941253653807E-5 34 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.03360565734628144 4.459941253653807E-5 35 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.044911608424293835 4.459941253653807E-5 36 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.06063290134342351 4.459941253653807E-5 37 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.07998904638428103 4.459941253653807E-5 38 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.10215495441494045 4.459941253653807E-5 39 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.1264393345410854 4.459941253653807E-5 40 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.1502331211293285 4.459941253653807E-5 41 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.1733356168232552 4.459941253653807E-5 42 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.197686896068205 4.459941253653807E-5 43 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.2241120479961038 4.459941253653807E-5 44 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.25401595410185257 4.459941253653807E-5 45 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.288781196174084 4.459941253653807E-5 46 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.325085117978826 4.459941253653807E-5 47 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.3631953159912978 4.459941253653807E-5 48 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.4032232887428407 4.459941253653807E-5 49 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.4439871518012365 4.459941253653807E-5 50 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.485888299879314 4.459941253653807E-5 51 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.5272988544194895 4.459941253653807E-5 52 0.0 6.689911880480711E-5 2.2299706268269035E-5 0.5706494834050045 4.459941253653807E-5 53 0.0 8.919882507307614E-5 2.2299706268269035E-5 0.6133757206150081 4.459941253653807E-5 54 0.0 8.919882507307614E-5 2.2299706268269035E-5 0.660450400547324 4.459941253653807E-5 55 0.0 8.919882507307614E-5 2.2299706268269035E-5 0.7133453038156582 4.459941253653807E-5 56 0.0 8.919882507307614E-5 2.2299706268269035E-5 0.7721496292450836 4.459941253653807E-5 57 0.0 8.919882507307614E-5 2.2299706268269035E-5 0.8315560467437523 4.459941253653807E-5 58 0.0 8.919882507307614E-5 2.2299706268269035E-5 0.8967826875784393 4.459941253653807E-5 59 0.0 8.919882507307614E-5 2.2299706268269035E-5 0.96415010021488 4.459941253653807E-5 60 0.0 8.919882507307614E-5 2.2299706268269035E-5 1.034929367910366 4.459941253653807E-5 61 0.0 8.919882507307614E-5 2.2299706268269035E-5 1.1040807570482682 4.459941253653807E-5 62 0.0 8.919882507307614E-5 2.2299706268269035E-5 1.1739011373742185 4.459941253653807E-5 63 0.0 8.919882507307614E-5 2.2299706268269035E-5 1.2475124677657745 4.459941253653807E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4010 0.0 36.163593 1 GTATCAA 8290 0.0 34.027824 1 TCAACGC 10770 0.0 25.501907 4 CAACGCA 11005 0.0 25.17766 5 TATCAAC 11080 0.0 25.066998 2 ATCAACG 10975 0.0 25.056442 3 AACGCAG 11410 0.0 24.413353 6 ACGCAGA 13345 0.0 20.616507 7 GTACATG 13525 0.0 20.24429 1 CGCAGAG 13710 0.0 19.994204 8 TACATGG 13860 0.0 19.516367 2 GAGTACT 7170 0.0 19.430986 12 AGAGTAC 13340 0.0 19.129076 11 ACATGGG 14285 0.0 18.356882 3 CGAATCG 310 3.6379788E-12 17.809155 37 CAGAGTA 14605 0.0 17.778378 10 GTACTTT 7790 0.0 17.752874 14 AGTACTT 7860 0.0 17.594574 13 GCAGAGT 15180 0.0 17.332113 9 GTACCGA 595 0.0 16.223434 6 >>END_MODULE