FastQCFastQC Report
Mon 6 Feb 2017
SRR2077265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4484364
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50750.039.3277241
GTATCAA104150.034.8729821
TATCAAC145050.024.956522
ATCAACG146350.024.618643
TCAACGC147200.024.4065574
CAACGCA150850.023.8719335
AACGCAG156200.023.0077766
GTACATG133000.019.4800111
ACGCAGA184050.019.4146187
CGCAGAG187250.018.9353338
GAGTACT122900.018.70925112
TACATGG134750.018.6535172
GTGGTAT26350.018.5096231
AGAGTAC183550.018.48984111
AGTACTT128750.017.90559613
GTACTTT129400.017.6220814
ACATGGG136450.017.4849593
CAGAGTA196850.017.45086110
GCAGAGT205150.016.946789
CGCGTAA1452.729503E-416.6512765
Produced by FastQC (version 0.11.5)