FastQCFastQC Report
Mon 6 Feb 2017
SRR2077264_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077264_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5267709
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86350.030.66011
GGTATCA48650.028.4512981
TCAACGC103450.025.0462844
ATCAACG104600.024.7028353
CAACGCA106750.024.2089945
TATCAAC110800.023.443752
AACGCAG111950.023.1580146
GTATAGC17150.021.334491
ACGCAGA127550.020.1920667
CGCAGAG130850.019.6058318
AGAGTAC122650.019.20258911
GTACATG122450.019.1686121
TACATGG119950.018.8658122
GAGTACT77400.018.6675812
ACATGGG122600.017.980723
TAGCCCT34950.017.5714134
CAGAGTA143100.017.0123210
GTACTTT84050.016.82343914
AGTACTT85850.016.79128513
CATGGGG78950.016.5186124
Produced by FastQC (version 0.11.5)