FastQCFastQC Report
Mon 6 Feb 2017
SRR2077264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5267709
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50750.034.007351
GTATCAA98400.032.26681
ATCAACG131350.023.9000433
TCAACGC131200.023.8746664
TATCAAC135500.023.2168712
CAACGCA136000.022.949085
AACGCAG140450.022.245916
ACGCAGA164350.018.8212837
GTACATG111450.018.6510241
TATACCG3001.8189894E-1218.413425
TACATGG109100.018.3149052
CGCAGAG168300.018.298898
AGAGTAC162650.017.91683411
GAGTACT121450.017.4843912
ACATGGG113350.016.6843283
AGTACTT129500.016.60293213
CAGAGTA181000.016.40562410
GCAGAGT187900.016.0411539
GTACTTT132500.016.03905314
CCTATAC11600.015.7964963
Produced by FastQC (version 0.11.5)