FastQCFastQC Report
Mon 6 Feb 2017
SRR2077263_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077263_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4603850
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76600.033.346791
GGTATCA38800.032.205341
TCAACGC97200.025.8017774
ATCAACG97250.025.7527563
TATCAAC101100.025.2481142
CAACGCA100050.025.1004545
AACGCAG105750.023.7630676
CGCGATT800.00291009121.55054512
GTACATG118350.021.2331371
ACGCAGA121500.020.5426037
TACATGG118850.020.2003292
CGCAGAG126600.019.6080538
AGAGTAC120150.019.25606311
GGACCGT5700.018.747456
GAGTACT72650.018.74734112
ACATGGG123100.018.6915173
CAGAGTA133450.017.49178510
GTACTTT79250.017.18810514
AGTACTT81100.016.88051413
GCAGAGT142500.016.7920579
Produced by FastQC (version 0.11.5)